Carbon compounds and their influence on in vitro growth and organogenesis

Welander, Margareta; Pawlicki, Nathalie

doi:10.1007/978-94-011-0790-7_9

Cited by 6 publications

(4 citation statements)

References 31 publications

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“…We anticipated that the presence of amino acids would improve quantitative as well as qualitative performance of lupin protoplasts in view of the fact that its phloem cells contain glutamine, asparagine and serine (Parsons & Baker, 1996). Welander & Pawlicki (1994) also adopted a similar approach of medium enrichment with compounds normally found in the species under study. Likewise, in L. angustifolius mesophyll protoplasts, an extract of L. luteus seeds improved protocolonisation (Schäfer‐Menuhr, 1987) due perhaps to its growth‐stimulating properties (Peretiatkowicz et al , 1994).…”

Section: Resultsmentioning

confidence: 99%

A breakthrough in lupin biotechnology: prolific protocolonisation in recalcitrant white lupin (Lupinus albus) triggered by bovine serum albumin

Sinha

Caligari

2009

Annals of Applied Biology

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Six nutrient formulations were studied for their efficacy in inducing mitosis in white lupin seedling cotyledon protoplasts of which the formulations of Schä fer-Menuhr & Stü rmer (AS) and Kao (K8p) were found to be superior over the other four when supplemented with 6-benzylaminopurine and a-naphthaleneacetic acid (a-NAA). An ultrafiltration treatment of K8p increased mitotic frequency by 130% when compared with the untreated control. Medium enrichment with 0.2% bovine serum albumin (BSA) brought about a dramatic 1341% rise in protoplast division in comparison with BSA-free medium but only when the enrichment was carried out in Kao and Michayluk (KM8p) background containing 2,4-dichlorophenoxyacetic acid, a-NAA and zeatin. A higher number of protocolonies (each proliferating from single protoplast following multiple divisions) were seen in 0.4% BSA. With this breakthrough in white lupin protoplast research, it is now possible to reproducibly obtain protocolonies that was hitherto not possible.

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Section: Resultsmentioning

confidence: 99%

A breakthrough in lupin biotechnology: prolific protocolonisation in recalcitrant white lupin (Lupinus albus) triggered by bovine serum albumin

Sinha

Caligari

2009

Annals of Applied Biology

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“…Nonetheless, the reactions of Eucalyptus vary against different carbon resources [154,156,164]. Glucose, as a reducing sugar, is able to perform diffusion across the cut surfaces of tissues and also metabolise in tissues [165]. A study on E. cloeziana in a culture medium supplemented with 15 g L −1 glucose presented the highest shoot number, high multiplication rate, and low death range due to a decrease in oxidation.…”

Section: Effect Of Carbohydratementioning

confidence: 99%

“…A study on E. cloeziana in a culture medium supplemented with 15 g L −1 glucose presented the highest shoot number, high multiplication rate, and low death range due to a decrease in oxidation. The higher level of some carbohydrate resources may lead to an increase in phenolic compounds and oxidation; however, it has been recommended that either using glucose or alternatives such as activated charcoal, ascorbic acid, citric acid, or other antioxidants in medium can decrease the oxidation level in Eucalyptus [58,145,165]. It has been reported that the combination of sucrose and glucose mainly influenced morphogenesis especially during cellular division in Eucalyptus [166].…”

Section: Effect Of Carbohydratementioning

confidence: 99%

The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp.

Abiri

Atabaki

Hamid

et al. 2020

Forests

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Micropropagation is a reliable technique in biotechnology and genetic engineering domain, which has been widely applied for rapid mass propagation of plants in vitro condition. Through micropropagation techniques, reproduction of plants can be attained from different explants using organogenesis and somatic embryogenesis. Over the decades, micropropagation techniques have offered tremendous potential for forest tree improvement. Eucalyptus is a woody plant species recalcitrant to in vitro culture. In general, the micropropagation of Eucalyptus culture processes and the genotype, environment surroundings, and age of explants in culture media is frequently linked with the occurrence of micropropagation variation. In the current review paper, an update of the most important physiological and molecular phenomena aspects of Eucalyptus micropropagation was linked to the most profound information. To achieve the mentioned target, the effect of plant growth regulators (PGRs), nutrients, other adjuvant and environmental features, as well as genetic interaction with morpho- and physiological mechanisms was studied from the induction to plant acclimatisation. On the other hand, important mechanisms behind the organogenesis and somatic embryogenesis of Eucalyptus are discussed. The information of current review paper will help researchers in choosing the optimum condition based on the scenario behind the tissue culture technique of Eucalyptus. However, more studies are required to identify and overcome some of the crucial bottlenecks in this economically important forest species to establish efficient micropropagation protocol at the industrial level.

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“…Carbohydrates have two main roles: (i) a carbon and energy source and (ii) an osmotic agent. Those sugars that are not metabolized can be used to alter the osmotic conditions in the tissue, which also seems to influence adventitious shoot and root formation (Welander, Pawlicki 1994). In addition to essential roles as substrates in carbon and energy metabolism and in polymer biosynthesis, sugars have important hormone-like functions as primary messengers in signal transduction (Rolland et al 2002).…”

Section: Discussionmentioning

confidence: 99%

Ex situ conservation of endangered plant species of Latvia by slow growth storage

2018

EEB

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Ex situ conservation of endangered plant species is the main objective of work of the Department of Ecophysiology of the National Botanic Garden of Latvia. In order to create a gene bank in tissue culture, use of appropriate methods for long-term preservation of living plant material is important. The objective of this study was to found ways to limit rate of shoot growth and to maximize the vitality period of shoot cultures. Several endangered plant species with high multiplication rate and sufficient number of explants were chosen for slow growth experiments. Murashige and Skoog medium supplemented with 1 to 6% carbohydrates (sucrose, sorbitol, mannitol), activated charcoal, and, for some species, cytokinin or other ingredients was used. After planting explants in experimental media, cultures were kept for four to five weeks at 25 °C, later at 5 °C. To evaluate explant growth in relation to various media conditions and genotype, root structure was analyzed using the program Win RHIZO 2002 C. Reduced shoot and root growth was observed for 23 species with sorbitol (for example, Armeria maritima, Galium tinctorium, Spergularia salina) and for eight species with mannitol (for example, Dianthus arenarius, Hydrocotile vulgaris, Juncus gerardii). Few species grew only on sucrose-containing medium. At 5 °C, most species survived for 24 and more months of storage without subcultivation. Some influence of storage medium composition on shoot first re-growth after storage was observed, but all cultures retained growth capacity after storage.

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Carbon compounds and their influence on in vitro growth and organogenesis

Cited by 6 publications

References 31 publications

A breakthrough in lupin biotechnology: prolific protocolonisation in recalcitrant white lupin (Lupinus albus) triggered by bovine serum albumin

A breakthrough in lupin biotechnology: prolific protocolonisation in recalcitrant white lupin (Lupinus albus) triggered by bovine serum albumin

The Prospect of Physiological Events Associated with the Micropropagation of Eucalyptus sp.

Ex situ conservation of endangered plant species of Latvia by slow growth storage

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