Carbon dioxide can inhibit biofilms formation and cellular properties of Shewanella putrefaciens at both 30 °C and 4 °C

Li, Peiyun; Mei, Jun; Xie, Jing

doi:10.1016/j.foodres.2022.111781

Cited by 12 publications

(9 citation statements)

References 47 publications

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“…A hole was punched in the center with an Oxford cup (Φ = 6 mm), and 70 μL of samples were added to the hole. Then, they were packaged in air or a pure CO 2 system and incubated at 4 °C for 24, 48, and 72 h. The diameter of the decomposition zone indicated the extracellular protease activity …”

Section: Methodsmentioning

confidence: 99%

“…The primary cause of food spoilage and subsequent massive amounts of waste is the metabolic activity of microbes Shewanella putrefaciens, a prevalent Gram-negative spoilage organism, shows strong spoilage potential even at low temperatures …”

Section: Introductionmentioning

confidence: 99%

“…CO 2 was extensively used to inhibit microorganisms for its nontoxic, eco-friendly, chemically inert, and inexpensive advantages . Our previous research studies , demonstrated that the spoilage potential of S.…”

Section: Introductionmentioning

confidence: 99%

“…putrefaciens targeting protein and flavor quality was efficiently restrained by CO 2 . According to Li et al, CO 2 can eventually produce hydrogen cations following series equilibria when it is dissolved in cellular fluids. The decreased intracellular pH can certainly exert adverse effects on enzyme activities that perform best only at neutral pH, and essential cellular metabolism processes can inevitably be inhibited.…”

Section: Introductionmentioning

confidence: 99%

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Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO₂ on Shewanella putrefaciens at 4 °C

Li,

Wang,

Xie

2024

J. Agric. Food Chem.

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To explore whether oxidative stress caused by 100% CO 2 is an inhibitory mechanism against Shewanella putrefaciens, the oxidative stress reaction, antioxidant activity, and damage to the cell membrane, protein, and DNA of CO 2 -incubated S. putrefaciens at 4 °C were evaluated. Research demonstrated that CO 2 caused more severe reactive oxygen species (ROS) accumulation. Simultaneously, weaker•− -scavenging activity and decreased T-VOC and GSH content were also observed. The activities of antioxidant enzymes (SOD, POD, CAT, and GPX) continuously declined, which might be attributed to the CO 2 -mediated decrease in the pH value. Correspondingly, the cell membrane was damaged with hyperpolarization, increased permeability, and more severe lipid peroxidation. The expression of total and membrane protein decreased, and the synthesis and activity of extracellular protease were inhibited. DNA was also subjected to oxidative damage and expressed at a lower level. All results collaboratively confirmed that ROS excitation and inhibition of antioxidant activity were important inhibition mechanisms of CO 2 on S. putrefaciens.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO₂ on Shewanella putrefaciens at 4 °C

Li,

Wang,

Xie

2024

J. Agric. Food Chem.

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“…CO2 has been used for fresh milk storage and preservation because it does not affect the nutritional content or flavor compared to conventional heat treatments. Also, CO2 is a natural compound that does not leave harmful residues in the product or the environment [11,12]. CO2 has been used for food preservation.…”

Section: Introductionmentioning

confidence: 99%

Deciphering the Mechanism by Which Carbon Dioxide Extends the Shelf Life of Raw Milk: A Microbiomics- and Metabolomics-Based Approach

Zheng,

Wei,

Liu

et al. 2023

Preprint

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Microbial community succession in raw milk determines its quality and storage period. In this study, carbon dioxide (CO2) at 2000 ppm was used to treat raw milk to investigate the mechanism of extending the shelf life of raw milk by CO2 treatment from the viewpoint of microbial colonies and metabolites. The results showed that the shelf life of CO2-treated raw milk was extended to 16 days at 4 ℃, while that of the control raw milk was only 6 days. Microbiomics analysis identified 221 Amplicon Sequence Variants (ASVs) in raw milk, and the alpha diversity of microbial communities increased (P < 0.05) with the extension of storage time. Among them, Pseudomonas, Actinobacteria and Serratia were the major microbial genus responsible for the deterioration of raw milk, with a percentage of 85.7%. A combined metagenomics and metabolomics analysis revealed that microorganisms altered the levels of metabolites, such as pyruvic acid, glutamic acid, 5'- cmp, arginine, 2-propenoic acid, and phenylalanine in the raw milk through metabolic activities, such as ABC transporters, pyrimidine metabolism, arginine and proline metabolism and phenylalanine metabolism, and reduced the shelf life of the raw milk. CO2 treatment prolonged the shelf life of the raw milk by inhibiting the abundance of Gram-negative aerobic bacteria, such as Acinetobacter guillouiae, Pseudomonas fluorescens, Serratia liquefaciens and Pseudomonas simiae.

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Bio-hydrogen production through microbial electrolysis cell: Structural components and influencing factors

Gautam

Nayak

Ress

et al. 2023

Chemical Engineering Journal

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Carbon dioxide can inhibit biofilms formation and cellular properties of Shewanella putrefaciens at both 30 °C and 4 °C

Cited by 12 publications

References 47 publications

Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO₂ on Shewanella putrefaciens at 4 °C

Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO₂ on Shewanella putrefaciens at 4 °C

Deciphering the Mechanism by Which Carbon Dioxide Extends the Shelf Life of Raw Milk: A Microbiomics- and Metabolomics-Based Approach

Bio-hydrogen production through microbial electrolysis cell: Structural components and influencing factors

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Carbon dioxide can inhibit biofilms formation and cellular properties of Shewanella putrefaciens at both 30 °C and 4 °C

Cited by 12 publications

References 47 publications

Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO2 on Shewanella putrefaciens at 4 °C

Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO2 on Shewanella putrefaciens at 4 °C

Deciphering the Mechanism by Which Carbon Dioxide Extends the Shelf Life of Raw Milk: A Microbiomics- and Metabolomics-Based Approach

Bio-hydrogen production through microbial electrolysis cell: Structural components and influencing factors

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Product

Resources

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Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO₂ on Shewanella putrefaciens at 4 °C

Excitation of Reactive Oxygen Species and Damage to the Cell Membrane, Protein, and DNA are Important Inhibition Mechanisms of CO₂ on Shewanella putrefaciens at 4 °C