Carbon dioxide pneumoperitoneum, intraperitoneal pressure, and peritoneal tissue hypoxia: a mouse study with controlled respiratory support

Matsuzaki, Sachiko; Jardon, К.; Maleysson, Elodie; D’Arpiany, Francis; Canis, Michel; Bazin, Jean-Étienne; Mage, G.

doi:10.1007/s00464-010-1069-z

Cited by 24 publications

(21 citation statements)

References 40 publications

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“…To further investigate whether the attenuated virulence is independent of NOX enzymatic activity, bacteria were administrated to an anaerobic niche, the peritoneum [39]. The survival rates of mice challenged with Δ nox WU2 were higher than those challenged with the WT parental strain both in BALB/c (Figure 2F; p <0.05) and CBA/N xid mice (Figure S3B in File S1).…”

Section: Resultsmentioning

confidence: 99%

NADH Oxidase Functions as an Adhesin in Streptococcus pneumoniae and Elicits a Protective Immune Response in Mice

et al. 2013

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The initial event in disease caused by S. pneumoniae is adhesion of the bacterium to respiratory epithelial cells, mediated by surface expressed molecules including cell-wall proteins. NADH oxidase (NOX), which reduces free oxygen to water in the cytoplasm, was identified in a non-lectin enriched pneumococcal cell-wall fraction. Recombinant NOX (rNOX) was screened with sera obtained longitudinally from children and demonstrated age-dependent immunogenicity. NOX ablation in S. pneumoniae significantly reduced bacterial adhesion to A549 epithelial cells in vitro and their virulence in the intranasal or intraperitoneal challenge models in mice, compared to the parental strain. Supplementation of Δnox WU2 with the nox gene restored its virulence. Saturation of A549 target cells with rNOX or neutralization of cell-wall residing NOX using anti-rNOX antiserum decreased adhesion to A549 cells. rNOX-binding phages inhibited bacterial adhesion. Moreover, peptides derived from the human proteins contactin 4, chondroitin 4 sulfotraferase and laminin5, homologous to the insert peptides in the neutralizing phages, inhibited bacterial adhesion to the A549 cells. Furthermore, rNOX immunization of mice elicited a protective immune response to intranasal or intraperitoneal S. pneumoniae challenge, whereas pneumococcal virulence was neutralized by anti-rNOX antiserum prior to intraperitoneal challenge. Our results suggest that in addition to its enzymatic activity, NOX contributes to S. pneumoniae virulence as a putative adhesin and thus peptides derived from its target molecules may be considered for the treatment of pneumococcal infections. Finally, rNOX elicited a protective immune response in both aerobic and anaerobic environments, which renders NOX a candidate for future pneumococcal vaccine.

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Section: Resultsmentioning

confidence: 99%

NADH Oxidase Functions as an Adhesin in Streptococcus pneumoniae and Elicits a Protective Immune Response in Mice

et al. 2013

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“…CO 2 dissolves in water to form carbonic acid which is suggested to lead to peritoneal acidosis [19]. Alternatively, if the gas is used at high pressure, it can increase airway pressure [10] and reduce cardiac output and peritoneal blood flow leading to acidosis, hypoxia, and oxidative stress [11, 20]. This is a particular concern when extrapolating experimental results from small animal models, where disproportionally high insufflation pressures are often used [10, 11].…”

Section: Discussionmentioning

confidence: 99%

“…The more recent literature indicates that pneumoperitoneal pressures ≥8 mmHg in a rat model correlate to high intraperitoneal pressures in humans, greater than the standard working pressures [10]. An excessively high intraperitoneal pressure may cause detachment of mesothelial cells by a mechanical effect, severe hypoxia, or both [11]. In addition, these studies fail to assess the effect of heated-only CO 2 on the peritoneum.…”

Section: Introductionmentioning

confidence: 99%

The Effects of Insufflation Conditions on Rat Mesothelium

Davey

Hayward

Marshall

et al. 2013

International Journal of Inflammation

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Aim. The aim of this investigation was to examine the alterations in the peritoneum after cold dry CO2, heated dry CO2, and humidified heated CO2 at pressures equivalent to intraperitoneal pressures used in human laparoscopy. Methods. Eighteen rats were divided into 4 treatment groups—group 1: untreated control; group 2: insufflation with cold dry CO2; group 3: insufflation with heated, dry CO2; group 4: insufflation with heated and humidified CO2. The abdomen was insufflated to 5 mm/Hg (flow rate 50 mL/min) for 2 h. Twelve hours later, tissue samples were collected for analysis by light microscopy (LM) and scanning electron microscopy (SEM). Results. Group 1: no abnormalities were detected. Group 2: specimens revealed an inflammatory response with loss of mesothelium and mesothelial cell nuclei showing lytic change. Cells were rounded with some areas of cell flattening and separation. Group 3: some animals showed little or no alteration, while others had a mild inflammatory response. Mesothelial cells were rounded and showed crenation on the exposed surface. Group 4: specimens showed little change from the control group. Conclusions. The LM results indicate that insufflations with heated, humidified CO2 are the least likely to induce mesothelial damage.

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“…High intraperitoneal CO 2 pressures of 15 mmHg can cause peritoneal hypoxia in a mouse model with controlled respiratory support [11]. Therefore, we investigated whether there was evidence of ischemia at the anastomotic site.…”

Section: Discussionmentioning

confidence: 99%

The influence of the CO2 pneumoperitoneum on a rat model of intestinal anastomosis healing

2011

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BackgroundThe CO2 pneumoperitoneum, which is used for laparoscopic surgery, causes local and systemic effects in patients. Concern arises about what the pressurized anoxic environment of the CO2 pneumoperitoneum has on intestinal healing. Earlier experimental work showed a negative correlation between intestinal healing and the applied intra-abdominal pressure. To further elucidate this, we developed a rat model, in which enterotomy healing can be compared after open or laparoscopic surgery. Possible mechanisms of injury, such as impaired neoangiogenesis or injury through hypoxia-induced pathways were studied.MethodsA new experimental mechanically ventilated rat model was developed. An enterotomy was made and closed via laparotomy (group I) or laparoscopy under CO2 pressures of 5 mmHg (group II) or 10 mmHg (group III). Intestinal healing was tested in vivo after 1 week by bursting-pressure analysis. The effect of the operative procedure on neoangiogenesis was tested by counting factor VIII positive vessels in biopsies of the perianastomotic granulation tissue after 1 week. Intestinal anoxia was tested by quantifying HIF-1α protein levels in intestinal biopsies, taken before the enterotomy closure.ResultsThe bursting pressures were significantly lower after laparoscopic surgery at 10 mmHg CO2 pneumoperitoneum (group III) compared with rats that had undergone open surgery (group I) or laparoscopic surgery at 5 mmHg CO2 pneumoperitoneum (group II). There was no significant quantitative difference between the three groups in the neoangiogenesis nor was there a difference in the amount of HIF-1α measured in the intestinal biopsies.ConclusionsWe developed a surgical model that is well fitted to study the effects of pneumoperitoneum on intestinal healing. With this model, we found further evidence of CO2 pressure-dependant hampered intestinal healing. These differences could not be explained by difference in neoangiogenesis nor local upregulation of hypoxic factors.

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Carbon dioxide pneumoperitoneum, intraperitoneal pressure, and peritoneal tissue hypoxia: a mouse study with controlled respiratory support

Abstract: The CO(2) pneumoperitoneum itself did not cause peritoneal hypoxia at either a tissue or a cellular level in a mouse model when a low IPP was used.

Cited by 24 publications

References 40 publications

NADH Oxidase Functions as an Adhesin in Streptococcus pneumoniae and Elicits a Protective Immune Response in Mice

NADH Oxidase Functions as an Adhesin in Streptococcus pneumoniae and Elicits a Protective Immune Response in Mice

The Effects of Insufflation Conditions on Rat Mesothelium

The influence of the CO2 pneumoperitoneum on a rat model of intestinal anastomosis healing

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