Carbon-dot-triggered aggregation/dispersion of gold nanoparticles for colorimetric detection of nucleic acids and its application in visualization of loop-mediated isothermal amplification

Abstract

In this study, cationic carbon dots (CDs) were prepared from p-phenylenediamine (pPDA) via a one-step hydrothermal method and used to trigger the aggregation and dispersion of gold nanoparticles (AuNPs) for...

Comprehensive review on fluorescent carbon dots and their applications in nucleic acid detection, nucleolus targeted imaging and gene delivery

“…4(5)). 100 The detection is based on the principle that negatively-charged nucleic acids can compete with AuNPs for binding to cationic CDs. In the absence of DNA, the CDs trigger the aggregation of AuNPs.…”

“…The reported assay exhibited a linear response within a DNA concentration range of 0.7–14 nM with a detection limit of 1.70 nM. 100 By tailoring the surface functionalities of CDs, researchers have demonstrated the preparation of CDs bearing cationic benzothiazolium groups and used them as a “turn-on” fluorescent probe for the sensitive detection of RNA with a detection limit of 0.62 μg mL −1 . 101 CDs generated from seaweed have been used as a fluorescent agent to visualize DNA in gel electrophoresis.…”