Buildings play a significant role in the rise of energy consumption and carbon emissions. Building operations are responsible for 28% of the world’s carbon emissions. It is crucial, therefore, to evaluate the environmental impact of various buildings’ operational phase in order to implement sustainable strategies for the mitigation of their energy usage and associated carbon footprint. While numerous studies have been conducted to determine the carbon footprint of conventional building operation phases, there are still a lack of actual data on the operational carbon (OC) emissions of mass timber buildings. There is also a lack of research pertaining to the operational carbon of buildings within larger campuses and their inherent energy usage. This study, therefore, aims to quantify empirical data on the carbon footprint of a mass timber building, using, as a case study, the recent Adohi Hall building, situated at the University of Arkansas, Fayetteville. The study also aims to examine and identify the best energy use scenarios for the campus building under consideration. The research team obtained data on Adohi Hall’s energy consumption, fuel input usage, and other utilities (such as water, electricity, chilled water, and natural gas) accounting for the operation of the building from 2021 to 2023, a span of three years. The University of Arkansas Facilities Management (FAMA) provided the data. The study relies on the life cycle assessment (LCA) as its primary approach, with SimaPro 9, Ecoinvent v3.7 database, DataSmart, version 2023.1 and the U.S. Life Cycle Inventory (USLCI) database utilized to model the energy and water consumption of Adohi Hall during the operational phase (B6 & B7). The results indicate 4496 kg CO2 eq emissions associated with the operation per square meter of Adohi Hall over its 50-year lifespan. The study also examines various scenarios of fuel sources leading to carbon emissions and provides insights into reduction strategies during the operational phase of buildings. Among them, the electricity based on a cleaner fuel source diversification, according to realistic expectations and technological advancements projections, results in a 17% reduction in Adohi Hall’s OC. Due to the usage of the combined heat and power (CHP) plant on the campus of the University of Arkansas as a complementary source of electricity and heating for Adohi Hall, the resulting carbon emission is approximately 21% (20.73%) less when compared to similar buildings in the same city but outside the campus. The study, therefore, reveals that CHP plant development is a highly effective strategy for building OC reduction.