Carbon felt-based bioelectrocatalytic flow-through detectors: Highly sensitive amperometric determination of H2O2 based on a direct electrochemistry of covalently modified horseradish peroxidase using cyanuric chloride as a linking agent

Wang, Yue; Hasebe, Yasushi

doi:10.1016/j.snb.2011.01.037

Cited by 24 publications

(14 citation statements)

References 42 publications

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“…Cyanuric chloride was anchored onto the electrode surface by immersing carbon cloth in 50 mM cyanuric chloride toluene solution for 24 h as previously described. 32 To improve the density of cyanuric chloride on the electrode surface, the reaction was performed at 0 C rather than room temperature.…”

Section: Electrode Modication Proceduresmentioning

confidence: 99%

Improved cathode materials for microbial electrosynthesis

Zhang

Nie

Bain

et al. 2013

Energy Environ. Sci.

361

298

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Microbial electrosynthesis is a promising strategy for the microbial conversion of carbon dioxide to transportation fuels and other organic commodities, but optimization of this process is required for commercialization. Cathodes which enhance electrode-microbe electron transfer might improve rates of product formation. To evaluate this possibility, biofilms of Sporomusa ovata, which are effective in acetate electrosynthesis, were grown on a range of cathode materials and acetate production was monitored over time. Modifications of carbon cloth that resulted in a positive-charge enhanced microbial electrosynthesis. Functionalization with chitosan or cyanuric chloride increased acetate production rates 6-7 fold and modification with 3-aminopropyltriethoxysilane gave rates 3-fold higher than untreated controls. A 3-fold increase in electrosynthesis over untreated carbon cloth cathodes was also achieved with polyaniline cathodes. However, not all strategies to provide positively charged surfaces were successful, as treatment of carbon cloth with melamine or ammonia gas did not stimulate acetate electrosynthesis. Treating carbon cloth with metal, in particular gold, palladium, or nickel nanoparticles, also promoted electrosynthesis, yielding electrosynthesis rates that were 6-, 4.7-or 4.5-fold faster than the untreated control, respectively. Cathodes comprised of cotton or polyester fabric treated with carbon nanotubes yielded cathodes that supported acetate electrosynthesis rates that were $3-fold higher than carbon cloth controls. Recovery of electrons consumed in acetate was $80% for all materials. The results demonstrate that one approach to increase rates of carbon dioxide reduction in microbial electrosynthesis is to modify cathode surfaces to improve microbe-electrode interactions. Broader contextMicrobial electrosynthesis is a recently conceived bioenergy strategy in which microorganisms use electrons derived from electrodes to reduce carbon dioxide to organic products that are excreted from the cells. Any form of electrical energy can power microbial electrosynthesis, but when electricity is obtained from solar technologies and water is the source of electrons, microbial electrosynthesis is an articial form of photosynthesis with many potential advantages over biomassbased energy strategies. This study demonstrates that there are several strategies for modifying cathode surface properties that can enhance rates of microbial electrosynthesis.

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Section: Electrode Modication Proceduresmentioning

confidence: 99%

Improved cathode materials for microbial electrosynthesis

Zhang

Nie

Bain

et al. 2013

Energy Environ. Sci.

361

298

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“…The electrocatalytic mechanism of HRP immobilized in the DNA/chitosan/Fe 3 O 4 /HRP film to H 2 O 2 reduction can be represented as follows [ 38 ]:…”

Section: Resultsmentioning

confidence: 99%

Direct Electrochemistry and Electrocatalysis of Horseradish Peroxidase Immobilized in a DNA/Chitosan-Fe3O4 Magnetic Nanoparticle Bio-Complex Film

Wang

Xia

et al. 2014

Materials

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A DNA/chitosan-Fe3O4 magnetic nanoparticle bio-complex film was constructed for the immobilization of horseradish peroxidase (HRP) on a glassy carbon electrode. HRP was simply mixed with DNA, chitosan and Fe3O4 nanoparticles, and then applied to the electrode surface to form an enzyme-incorporated polyion complex film. Scanning electron microscopy (SEM) was used to study the surface features of DNA/chitosan/Fe3O4/HRP layer. The results of electrochemical impedance spectroscopy (EIS) show that Fe3O4 and enzyme were successfully immobilized on the electrode surface by the DNA/chitosan bio-polyion complex membrane. Direct electron transfer (DET) and bioelectrocatalysis of HRP in the DNA/chitosan/Fe3O4 film were investigated by cyclic voltammetry (CV) and constant potential amperometry. The HRP-immobilized electrode was found to undergo DET and exhibited a fast electron transfer rate constant of 3.7 s−1. The CV results showed that the modified electrode gave rise to well-defined peaks in phosphate buffer, corresponding to the electrochemical redox reaction between HRP(Fe(III)) and HRP(Fe(II)). The obtained electrode also displayed an electrocatalytic reduction behavior towards H2O2. The resulting DNA/chitosan/Fe3O4/HRP/glassy carbon electrode (GCE) shows a high sensitivity (20.8 A·cm−2·M−1) toward H2O2. A linear response to H2O2 measurement was obtained over the range from 2 μM to 100 μM (R2 = 0.99) and an amperometric detection limit of 1 μM (S/N = 3). The apparent Michaelis-Menten constant of HRP immobilized on the electrode was 0.28 mM. Furthermore, the electrode exhibits both good operational stability and storage stability.

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“…(5)(6)(7) Electrochemistry has proved to be effective for H 2 O 2 determination. Redox proteins including horseradish peroxidase, (8,9) myoglobin, (10) hemoglobin, (11) and cytochrome c (12) have been used for the fabrication of H 2 O 2 biosensors. However, enzymatic sensors usually suffer from the effects of temperature, pH, and toxic compounds because of the intrinsic nature of enzymes.…”

Section: Introductionmentioning

confidence: 99%

Carbon-black-doped Polyimide-modified Glassy Carbon Electrode for Sensitive Nonenzymatic Amperometric Determination of Hydrogen Peroxide

Wang¹,

Chen²,

Zhang³

et al. 2019

Sensors and Materials

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In this study, a novel nonenzymatic sensor for the determination of hydrogen peroxide (H 2 O 2) was developed. The direct electrocatalytic oxidation of H 2 O 2 in an aqueous medium at a carbon-black (CB)-doped self-made polyimide (PI)-modified glassy carbon electrode (GCE) was investigated. The fabrication of this electrode is simple and reproducible. The filmlike surface was prepared by directly mixing the CB and PI solution. Under optimized conditions, the linear range of H 2 O 2 is from 6 to 10000 µM with the detection limit of 1 µM. The electrode exhibited high stability, excellent sensitivity, stable reproducibility, and fast response time (less than 5 s). The results show that PI can be used as a reliable and long-life sensor for hydrogen peroxide detection, exhibiting a new application for the PI.

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Carbon felt-based bioelectrocatalytic flow-through detectors: Highly sensitive amperometric determination of H2O2 based on a direct electrochemistry of covalently modified horseradish peroxidase using cyanuric chloride as a linking agent

Cited by 24 publications

References 42 publications

Improved cathode materials for microbial electrosynthesis

Improved cathode materials for microbial electrosynthesis

Direct Electrochemistry and Electrocatalysis of Horseradish Peroxidase Immobilized in a DNA/Chitosan-Fe3O4 Magnetic Nanoparticle Bio-Complex Film

Carbon-black-doped Polyimide-modified Glassy Carbon Electrode for Sensitive Nonenzymatic Amperometric Determination of Hydrogen Peroxide

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