Carbon Fiber-based Microelectrodes and Microbiosensors

Budai, Dénes

doi:10.5772/7158

Cited by 16 publications

(14 citation statements)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Carbon fiber monofilaments demonstrate outstanding physicochemical properties and serve as excellent base electrodes on a micrometer scale. In microbiosensors, the carbon tip is covered with biological sensing elements such as an enzyme, receptor protein, antibody, or nucleic acid immobilized in a conducting polymer matrix [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

A novel carbon tipped single micro-optrode for combined optogenetics and electrophysiology

et al. 2018

View full text Add to dashboard Cite

Optical microelectrodes (optrodes) are used in neuroscience to transmit light into the brain of a genetically modified animal to evoke and record electrical activity from light-sensitive neurons. Our novel micro-optrode solution integrates a light-transmitting 125 micrometer optical fiber and a 9 micrometer carbon monofilament to form an electrical lead element, which is contained in a borosilicate glass sheathing coaxial arrangement ending with a micrometer-sized carbon tip. This novel unit design is stiff and slender enough to be used for targeting deep brain areas, and may cause less tissue damage compared with previous models. The center-positioned carbon fiber is less prone to light-induced artifacts than side-lit metal microelectrodes previously presented. The carbon tip is capable of not only recording electrical signals of neuronal origin but can also provide valuable surface area for electron transfer, which is essential in electrochemical (voltammetry, amperometry) or microbiosensor applications. We present details of design and manufacture as well as operational examples of the newly developed single micro-optrode, which includes assessments of 1) carbon tip length–impedance relationship, 2) light transmission capabilities, 3) photoelectric artifacts in carbon fibers, 4) responses to dopamine using fast-scan cyclic voltammetry in vivo, and 5) optogenetic stimulation and spike or local field potential recording from the rat brain transfected with channelrhodopsin-2. With this work, we demonstrate that our novel carbon tipped single micro-optrode may open up new avenues for use in optogenetic stimulation when needing to be combined with extracellular recording, electrochemical, or microbiosensor measurements performed on a millisecond basis.

show abstract

Section: Introductionmentioning

confidence: 99%

A novel carbon tipped single micro-optrode for combined optogenetics and electrophysiology

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Although microelectrodes have unparalleled temporal response, microdialysis can take minutes. Conversely, microdialysis can specifically identify ACh after extraction, but microelectrodes are more suitable for in vivo detection of catecholamines than inoxidable ACh 8 . The last decade has witnessed considerable progress in improving microdialysis and microeletrodes for neurotransmitter studies.…”

Section: Introductionmentioning

confidence: 99%

A DNA-based optical nanosensor forin vivoimaging of acetylcholine in the peripheral nervous system

Xia

Yang

et al. 2020

Preprint

View full text Add to dashboard Cite

AbstractThe ability to monitor the release of neurotransmitters during synaptic transmission would significantly impact the diagnosis and treatment of neurological disease. Here, we present a DNA-based enzymatic nanosensor for quantitative detection of acetylcholine (ACh) in the peripheral nervous system of living mice. ACh nanosensors consist of DNA as a scaffold, acetylcholinesterase as a recognition component, pH-sensitive fluorophores as signal generators, and α-bungarotoxin as a targeting moiety. We demonstrate the utility of the nanosensors in the submandibular ganglia of living mice to sensitively detect ACh ranging from 0.228 μM to 358 μM. In addition, the sensor response upon electrical stimulation of the efferent nerve is dose-dependent, reversible, and we observe a reduction of ~76% in sensor signal upon pharmacological inhibition of ACh release. Equipped with an advanced imaging processing tool, we further spatially resolve ACh signal propagation on the tissue level. Our platform enables sensitive measurement and mapping of ACh transmission in the peripheral nervous system.

show abstract

“…CF has several mechanical and electrical properties and is an excellent electrode for electrophysiological and electrochemical measurements and has been used since 1979. It has been used for electro-chemical detection of catecholamines, ACh, Ch, glucose and Glu [ 9 ]. A recent alternative to monitor neurotransmitters with high temporal resolution involves biosensors to design enzymatic reactions to generate a “derivate” from a neurotransmitter as a substrate that could be measured by luminesce [ 10 , 11 ], electrochemistry [ 5 ] and fluorescence [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

An Update of the Classical and Novel Methods Used for Measuring Fast Neurotransmitters During Normal and Brain Altered Function

Vh¹,

Cl²,

Jc³

et al. 2015

View full text Add to dashboard Cite

To understand better the cerebral functions, several methods have been developed to study the brain activity, they could be related with morphological, electrophysiological, molecular and neurochemical techniques. Monitoring neurotransmitter concentration is a key role to know better how the brain works during normal or pathological conditions, as well as for studying the changes in neurotransmitter concentration with the use of several drugs that could affect or reestablish the normal brain activity. Immediate response of the brain to environmental conditions is related with the release of the fast acting neurotransmission by glutamate (Glu), γ-aminobutyric acid (GABA) and acetylcholine (ACh) through the opening of ligand-operated ion channels. Neurotransmitter release is mainly determined by the classical microdialysis technique, this is generally coupled to high performance liquid chromatography (HPLC). Detection of neurotransmitters can be done by fluorescence, optical density, electrochemistry or other detection systems more sophisticated. Although the microdialysis method is the golden technique to monitor the brain neurotransmitters, it has a poor temporal resolution. Recently, with the use of biosensor the drawback of temporal resolution has been improved considerably, however other inconveniences have merged, such as stability, reproducibility and the lack of reliable biosensors mainly for GABA. The aim of this review is to show the important advances in the different ways to measure neurotransmitter concentrations; both with the use of classic techniques as well as with the novel methods and alternant approaches to improve the temporal resolution.

show abstract

Carbon Fiber-based Microelectrodes and Microbiosensors

Cited by 16 publications

References 63 publications

A novel carbon tipped single micro-optrode for combined optogenetics and electrophysiology

A novel carbon tipped single micro-optrode for combined optogenetics and electrophysiology

A DNA-based optical nanosensor forin vivoimaging of acetylcholine in the peripheral nervous system

An Update of the Classical and Novel Methods Used for Measuring Fast Neurotransmitters During Normal and Brain Altered Function

Contact Info

Product

Resources

About