Carbon ion (12C6+) irradiation induces the expression of Klrk1 in lung cancer and optimizes the tumor microenvironment based on the NKG2D/NKG2D-Ls pathway

Wang, Jiangtao; Dai, Ziying; Miao, Yifei; Zhao, Ting; Gan, Jian; Zhao, Chengpeng; Ran, Juntao; Guan, Quan-Lin

doi:10.1016/j.canlet.2021.09.003

Cited by 12 publications

(8 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The expression levels of IL12A [ 10 ] and KIR3DL2 [ 45 ] in lung adenocarcinoma, and KIR3DL2 in lung squamous cell carcinoma were significantly lower than in adjacent normal tissues and decreased as the clinical stage increased. KLRK1 expression in NSCLC was significantly lower than in normal tissues, indicating a prognostic gene [ 46 ].…”

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiles of Multiple Synchronous Lesions in Lung Adenocarcinoma

Lim

Han

Park

et al. 2021

Cells

View full text Add to dashboard Cite

Many studies support a stepwise continuum of morphologic changes between atypical adenomatous hyperplasia (AAH) and lung adenocarcinoma (ADC). Here we characterized gene expression patterns and the association of differentially expressed genes and immune tumor microenvironment behaviors in AAH to ADC during ADC development. Tumor tissues from nine patients with ADC and synchronous multiple ground glass nodules/lesions (GGN/Ls) were analyzed using RNA sequencing. Using clustering, we identified genes differentially and sequentially expressed in AAH and ADC compared to normal tissues. Functional enrichment analysis using gene ontology terms was performed, and the fraction of immune cell types was estimated. We identified up-regulated genes (ACSL5 and SERINC2) with a stepwise change of expression from AAH to ADC and validated those expressions by quantitative PCR and immunohistochemistry. The immune cell profiles revealed increased B cell activities and decreased natural killer cell activities in AAH and ADC. A stepwise change of differential expression during ADC development revealed potential effects on immune function in synchronous precursors and in tumor lesions in patients with lung cancer.

show abstract

Section: Discussionmentioning

confidence: 99%

Gene Expression Profiles of Multiple Synchronous Lesions in Lung Adenocarcinoma

Lim

Han

Park

et al. 2021

Cells

View full text Add to dashboard Cite

show abstract

“…Wang et al 24 found that CIRT induces Klrk1 gene expression and activates the NKG2D/NKG2D-Ls pathway, thereby improving the infiltration and functional status of NK cells. A study by König et al 25 on DCs found that phenotypic maturation remained unchanged after carbon ion irradiation, and functionalities such as phagocytosis, migration, and cytokine secretion remained unaffected, indicating a possible persistent potential for inducing an adaptive immune response.…”

Section: The Effects Of Cirt On the Immune Systemmentioning

confidence: 99%

“…35 Preclinical studies on the combination of CIRT and Treg inhibitor therapy are ongoing. Wang et al 24 found that CIRT combined with Treg inhibitor therapy enhanced the infiltration and function of NK cells in the tumor microenvironment of lung cancer and prolonged the survival time of mice.…”

Section: Preclinical Studiesmentioning

confidence: 99%

Clinical application of high‐LET radiotherapy combined with immunotherapy in malignant tumors

Meng,

2024

Precision Radiation Oncology

View full text Add to dashboard Cite

The superior physical and biological properties of high linear energy transfer (LET), as opposed to traditional low‐LET rays, underscore the advantage of proton therapy (PRT) and carbon ion radiotherapy (CIRT) are better than traditional photon radiotherapy (XRT). With the advancements in science, an increasing number of hospitals have introduced new technologies. However, radiotherapy is primarily used for local treatment, which means that if the tumor has metastasized to distant sites, it is often necessary to combine it with systemic therapies such as immunotherapy. In recent years, the combination of high‐LET radiotherapy and immunotherapy has emerged as a promising treatment option in oncology and many studies have confirmed its efficacy for both local and distant metastases. In this review, we summarize the effects of PRT and CIRT on the immune system in detail, followed by an introduction to preclinical and clinical studies of PRT and CIRT in combination with immune checkpoint inhibitor (ICIs) therapy. We also briefly introduce some preclinical studies on CIRT in combination with dendritic cells (DCs) and Treg inhibitor therapies.

show abstract

“…These macrophage-derived mediators have been recognized for their cytotoxic activities against cancer cells [14]. Natural killer (NK) cells are immune cells involved in innate immunity and possess various receptors that recognize abnormal cells, including infected cells or tumor cells [15][16][17]. The interaction between macrophages and NK cells is a critical initial line of defense against pathogens including viruses, bacteria, parasites, and fungi.…”

Section: Introductionmentioning

confidence: 99%

NK cell-mediated immunostimulatory effects of ethanol extract of Morinda citrifolia (noni) fruit

Kim

Rahmawati

Hong

et al. 2022

BMC Complement Med Ther

View full text Add to dashboard Cite

Background Morinda citrifolia (Noni) is a plant that has long been used in various products such as foods and cosmetics. Although noni has been known to have immunostimulatory activity, detailed mechanism at the cellular level has not been fully elucidated yet. In this study, we focused on understanding as to how noni fruit can positively stimulate body’s immune responses. Methods To do this, an ethanol extract of noni fruit (Mc-fEE) was prepared and administered for 30 days to male C57BL/6 mice for in vivo experiment. NK cell activity and cytokine production level from Mc-fEE-treated mice were analyzed by flowcytometry, real-time PCR, and ELISA. Mc-fEE-triggered molecular events were detected from RAW264.7 cells and splenocytes using Western blotting and real-time PCR analyses. Results The mRNA expression levels of cytokines such as interleukin families, interferon (IFN)-β, and tumor necrosis factor (TNF)-α were increased by Mc-fEE treatment in vitro and in vivo. Western blotting analysis showed that the phosphorylation levels of nuclear factor (NF)-κB and activator protein (AP)-1 subunits these were enhanced in Mc-fEE-treated RAW264.7 cells. In addition, according to in vivo experiments, it was considered that Mc-fEE can increase the population of splenic NK cells and subsequent upregulation of their cytotoxic activity against YAC-1 cells, a T- cell lymphoma. Conclusion In this paper, we could confirm that Mc-fEE has remarkable immunostimulatory effects by activation and increase of the NK cell population.

show abstract

Carbon ion (12C6+) irradiation induces the expression of Klrk1 in lung cancer and optimizes the tumor microenvironment based on the NKG2D/NKG2D-Ls pathway

Cited by 12 publications

References 50 publications

Gene Expression Profiles of Multiple Synchronous Lesions in Lung Adenocarcinoma

Gene Expression Profiles of Multiple Synchronous Lesions in Lung Adenocarcinoma

Clinical application of high‐LET radiotherapy combined with immunotherapy in malignant tumors

NK cell-mediated immunostimulatory effects of ethanol extract of Morinda citrifolia (noni) fruit

Contact Info

Product

Resources

About