Carbon metabolism inSulfobacillus thermosulfidooxidans subsp.asporogenes, strain 41

Цаплина, И. А.; Krasil’nikova, E. N.; Zakharchuk, L. M.; Егорова, М.А.; Bogdanova, T. I.; Karavaiko, G. I.

doi:10.1007/bf02756732

Cited by 15 publications

(13 citation statements)

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“…Although members of the genus Sulfobacillus can grow in autotrophic conditions, the largest growth rates are observed mixotrophically [18], unlike the obligate chemolithoautotroph A. ferrooxidans. During S. thermosulfidooxidans mixotrophic growth, the activity of the phosphoenolpyruvate (PEP) carboxylase enzyme is improved and is the highest among four different enzymes studied by Tsaplina et al [19]. Conversely, the activity of the ribulose bisphosphate carboxylase (RuBPC) enzyme predominates during autotrophic growth and an increase on carbon dioxide content in the air fed to the system is needed for stable growth.…”

Section: Resultsmentioning

confidence: 99%

Kinetics of ferrous iron oxidation by Sulfobacillus thermosulfidooxidans

Piña

Oliveira

Cruz

et al. 2010

Biochemical Engineering Journal

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The biological oxidation of ferrous iron is an important sub-process in the bioleaching of metal sulfides and other bioprocesses such as the removal of H 2 S from gases, the desulfurization of coal and the treatment of acid mine drainage (AMD). As a consequence, many Fe(II) oxidation kinetics studies have mostly been carried out with mesophilic microorganisms, but only a few with moderately thermophilic microorganisms. In this work, the ferrous iron oxidation kinetics in the presence of Sulfobacillus thermosulfidooxidans (DSMZ 9293) was studied. The experiments were carried out in batch mode (2L STR) and the effect of the initial ferrous iron concentration (2-20 g L −1) on both the substrate consumption and bacterial growth rate was assessed. The Monod equation was applied to describe the growth kinetics of this microorganism and values of max and K s of 0.242 h −1 and 0.396 g L −1 , respectively, were achieved. Due to the higher temperature oxidation, potential benefits on leaching kinetics are forecasted.

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Section: Resultsmentioning

confidence: 99%

Kinetics of ferrous iron oxidation by Sulfobacillus thermosulfidooxidans

Piña

Oliveira

Cruz

et al. 2010

Biochemical Engineering Journal

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“…Most noticeable growth of strain K1 T (up to 30 mg protein l 21 ) was observed in the presence of 0?1 % (w/v) complex organic substrates -peptone, yeast extract and casein hydrolysate -which was accompanied by the excretion of 0?5-1?2610 23 units proteolytic enzymes ml 21 , determined according to Nomoto & Narahashi (1956). Much like with the sulfobacilli (Wood & Kelly, 1983;Zakharchuk et al, 1994Zakharchuk et al, , 2003Tsaplina et al, 1994Tsaplina et al, , 2000, mixotrophic growth conditions were optimal for strain K1 T ; the most active growth and the highest biomass yield were observed under these conditions (see After a change in the cultivation conditions from mixotrophic to heterotrophic in the presence of glucose and yeast extract in the medium (first culture transfer), strain K1 T used 60 % of the glucose initially present (1?2 mM) in the medium (see Fig. B, available in IJSEM Online).…”

mentioning

confidence: 98%

“…In sulfobacilli, the TCA cycle is blocked at the level of 2-oxoglutarate dehydrogenase, thus merely fulfilling a biosynthetic role (Zakharchuk et al, 1994(Zakharchuk et al, , 2003Tsaplina et al, 2000). Some strains of sulfobacilli are capable of oxidoreduction of iron (Bridge & Johnson, 1998).…”

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confidence: 99%

Reclassification of ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended description of the genus Alicyclobacillus

Karavaiko

Bogdanova

Tourova

et al. 2005

International Journal of Systematic and Evolutionary Microbiology

109

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Comparative analysis of 16S rRNA gene sequences, DNA–DNA hybridization data and phenotypic properties revealed that ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 is not a member of the genus Sulfobacillus. Phylogenetically, strain K1 is closely related to unclassified strains of the genus Alicyclobacillus: the 16S rRNA gene sequence of strain K1 is similar to that of Alicyclobacillus sp. AGC-2 (99·6 %), Alicyclobacillus sp. 5C (98·9 %) and Alicyclobacillus sp. CLG (98·6 %) and bacterium GSM (99·1 %). The 16S rRNA gene sequence similarity values for strain K1 and species of the genus Alicyclobacillus with validly published names were in the range 92·1–94·6 %, and for S. thermosulfidooxidans VKM B-1269T the value was 87·7 %. Sulfobacillus disulfidooxidans SD-11T was also phylogenetically related to strain K1 (92·6 % sequence similarity) and thus belonged to the genus Alicyclobacillus. Chemotaxonomic data, such as the major cell-membrane lipid components of strains K1 and SD-11T (ω-alicyclic fatty acids) and the major isoprenoid quinone (menaquinone MK-7) of strain K1, supported the affiliation of strains K1 and SD-11T to the genus Alicyclobacillus. Physiological and molecular biological tests allowed genotypic and phenotypic differentiation of strains K1 and SD-11T from the nine Alicyclobacillus species with validly published names. The G+C content of the DNA of strain K1 was 48·7±0·6 mol%; that of strain SD-11T was 53±1 mol%. DNA–DNA reassociation studies showed low relatedness (22 %) between strains K1 and SD-11T, and even lower relatedness (3–5 %) between these strains and Alicyclobacillus acidocaldarius subsp. acidocaldarius ATCC 27009T, DSM 446T. DNA reassociation of strains K1 and SD-11T with Alicyclobacillus cycloheptanicus DSM 4006T gave values of 15 and 21, respectively. Based on the phenotypic and phylogenetic characteristics of strains K1 and SD-11T, Alicyclobacillus tolerans sp. nov. (type strain, K1T=VKM B-2304T=DSM 16297T) and Alicyclobacillus disulfidooxidans comb. nov. (type strain, SD-11T=ATCC 51911T=DSM 12064T) are proposed.

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“…The reason was that the cells of chemolithoautotrophs lacked the relevant enzyme systems [15,16]. Upon addition of the easily utilizable YE to the pulp, the microbial community still failed to utilize some of the organic substances of the medium, even though organic compounds could support the growth of the mixotrophic components of the community (sulfoba cilli and archaea) [1,14]. The C org content increased in the control variant, and this provided evidence that the increase was due to the presence of organic exome tabolites in the liquid phase.…”

Section: Discussionmentioning

confidence: 99%

“…Upon addition of organic substances, the rates of oxida tion of the mineral substrate and growth processes signif icantly increase [14]. Moreover, heterotrophic organisms are also present in the community.…”

Section: Requirement In Organic Substancesmentioning

confidence: 99%

Biooxidation of a gold-containing sulfide concentrate in relation to changes in physical and chemical conditions

et al. 2012

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288Optimizing the technological process related vari ables is a prerequisite for increasing the rate of biolog ical oxidation of sulfide minerals and the degree of recovery of noble metals. The temperature and pH val ues and the requirement in organic substrates are the most important factors influencing the growth and energy metabolism of microorganisms, their develop mental dynamics, succession of their dominant groups in the microbial community, and efficiency in leach ing/oxidizing the mineral components of ore concen trates.According to the literature, sulfur oxidizers (Acidithiobacillus caldus, Acidithiobacillus thiooxidans, and Sulfobacillus species) prevail during the initial stages of leaching gold , copper , and nickel contain ing sulfide ore concentrates at mesophilic (30 and 35°C) and moderately thermophilic conditions (39 and 45°C). During the intermediate and the late stages of the process, sulfur oxidizers are replaced by iron oxidizers. As a rule, Leptospirillum ferriphilum is the dominant species [1][2][3][4]. Raising the temperature results in decreasing Acidithiobacillus numbers and increasing the Sulfobacillus share, while retaining the dominance of the leptospirilli. Decreasing the pH to 1.2-1.0 gives an advantage to the microorganisms resistant to high acidity values. These are L. ferriphi lum and some mixotrophs similar to the Ferroplasma archaea, whose growth is activated by the acid induced lysis of a part of the cells of the microbial community and, accordingly, the release of C org into the medium liquid phase [1]. The presence of het erotrophic cultures in the community, such as Alicy clobacillus spp., Ferroplasma spp., and Ferrimicrobium acidiphilum, increases the degree and rate of pyrite leaching and oxidation [1,2,5,6]. For instance, addi tion of an organic substrate (0.005-0.05% of yeast extract) significantly influenced the succession of the predominant species in the community and stimulated the leaching and oxidation of chalcopyrite upon rais ing the temperature to 40-60°C [4] and of pyrite at 30°C [6]. Hence the most efficient community includes Fe 2+ , S 2-/S 0 oxidizers and heterotrophic microorganisms.The goal of this work was to elucidate the influence of (i) the pulp temperature and pH values maintained during the biooxidation of ore flotation concentrate and (ii) yeast extract induced changes in the medium composition on the process related variables during the growth dynamics of the microbial community. MATERIALS AND METHODSResearch subject. An enrichment culture of micro organisms was obtained from ore flotation concentrate at 35-37°C. The microbial community used as inoc ulum was supplemented with bacteria and archaea iso lated from pulp samples from the reactor of the gold recovery plant of the Polyus Closed Joint stock Com pany and with the following cultures from the collec tion of cultures of chemolithotrophic microorganisms Abstract-The growth of a microbial community and the oxidation of iron and sulfur containing substrates in batch culture during the leaching/...

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Carbon metabolism inSulfobacillus thermosulfidooxidans subsp.asporogenes, strain 41

Cited by 15 publications

References 10 publications

Kinetics of ferrous iron oxidation by Sulfobacillus thermosulfidooxidans

Kinetics of ferrous iron oxidation by Sulfobacillus thermosulfidooxidans

Reclassification of ‘Sulfobacillus thermosulfidooxidans subsp. thermotolerans’ strain K1 as Alicyclobacillus tolerans sp. nov. and Sulfobacillus disulfidooxidans Dufresne et al. 1996 as Alicyclobacillus disulfidooxidans comb. nov., and emended description of the genus Alicyclobacillus

Biooxidation of a gold-containing sulfide concentrate in relation to changes in physical and chemical conditions

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