Carbon Monoxide Enhances Human Neutrophil Migration in a Cyclic GMP-dependent Way

Vanuffelen, B. E.; Koster, Ben M. de; VanSteveninck, John; Elferink, J. G. R.

doi:10.1006/bbrc.1996.1305

Cited by 42 publications

(27 citation statements)

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“…PKG is required for plasmin-and a-elastin-stimulated migration of monocytes and macrophages, respectively (Kamisato et al, 1997;Uemura and Okamoto, 1997), and PKG mediates neutrophil motility induced by carbon monoxide and endothelin-1/endothelin-3 (Elferink and De Koster, 1998;VanUffelen et al, 1996). Yet, the mechanisms underlying PKG-dependent increase in migration of immune cells are unknown.…”

Section: Discussionmentioning

confidence: 99%

cGMP-dependent Protein Kinase Iβ Regulates Breast Cancer Cell Migration and Invasion via a Novel Interaction with the Actin/Myosin-associated Protein Caldesmon

Schwappacher

Rangaswami

Su-Yuo

et al. 2013

Journal of Cell Science

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SummaryThe two isoforms of type I cGMP-dependent protein kinase (PKGIa and PKGIb) differ in their first ,100 amino acids, giving each isoform unique dimerization and autoinhibitory domains. The dimerization domains form coiled-coil structures and serve as platforms for isoform-specific protein-protein interactions. Using the PKGIb dimerization domain as an affinity probe in a proteomic screen, we identified the actin/myosin-associated protein caldesmon (CaD) as a PKGIb-specific binding protein. PKGIb phosphorylated human CaD on serine 12 in vitro and in intact cells. Phosphorylation on serine 12 or mutation of serine 12 to glutamic acid (S12E) reduced the interaction between CaD and myosin IIA. Because CaD inhibits myosin ATPase activity and regulates cell motility, we examined the effects of PKGIb and CaD on cell migration and invasion. Inhibition of the NO/cGMP/PKG pathway reduced migration and invasion of human breast cancer cells, whereas PKG activation enhanced their motility and invasion. siRNA-mediated knockdown of endogenous CaD had pro-migratory and pro-invasive effects in human breast cancer cells. Reconstituting cells with wild-type CaD slowed migration and invasion; however, CaD containing a phospho-mimetic S12E mutation failed to reverse the pro-migratory and pro-invasive activity of CaD depletion. Our data suggest that PKGIb enhances breast cancer cell motility and invasive capacity, at least in part, by phosphorylating CaD. These findings identify a pro-migratory and pro-invasive function for PKGIb in human breast cancer cells, suggesting that PKGIb is a potential target for breast cancer treatment.

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Section: Discussionmentioning

confidence: 99%

cGMP-dependent Protein Kinase Iβ Regulates Breast Cancer Cell Migration and Invasion via a Novel Interaction with the Actin/Myosin-associated Protein Caldesmon

Schwappacher

Rangaswami

Su-Yuo

et al. 2013

Journal of Cell Science

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“…The catalytic by-products, CO, iron, and bilirubin, have been demonstrated to mediate anti-inflammatory, antioxidative, and antiapoptotic effects [2,[12][13][14][15]. CO was identified to suppress inflammatory cytokines and influence neutrophil migration [16,17]. Biliverdin and bilirubin have been shown to inhibit monocyte chemotaxis and complement activation [18,19].…”

Section: Discussionmentioning

confidence: 99%

Heme Oxygenase-1 Promoter Polymorphism Protects Liver Allograft

Zhang

Guan

et al. 2015

Indian J Surg

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Heme oxygenase-1 has been identified to protect allograft from ischemia/reperfusion and immunologic rejection. Activity of heme oxygenase-1 is regulated by a guanine-thymine dinucleotide length polymorphism in the heme oxygenase-1 gene promoter. In this study, we aimed to explore the impact of the heme oxygenase-1 gene promoter polymorphism of donors and recipients on the orthotopic liver graft function after transplantation. Sixty recipients and their accompanying donors of orthotopic liver allografts were included retrospectively in this study. Heme oxygenase-1 gene promoter polymorphism was assessed using genomic DNA isolated from cryopreserved splenocytes or peripheral blood mononuclear cells and analyzed by genetic analyzer. Small allele of the donor heme oxygenase-1 gene polymorphism significantly prolonged the graft survival (p=0.017). Recipients of allografts from a class of small-allele carrier had significantly lower serum total bilirubin compared with recipients of a nonclass small-allele donor liver (p < 0.01).Additionally, in recipients of small-carrier allografts, cold ischemia time (<10 h or ≥10 h) did not affect the total bilirubin significantly. Our study suggested a protective function of donor-derived heme oxygenase-1 gene promoter polymorphism on orthotopic liver allograft function after transplantation.

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“…The NO x level is affected not only by endogenous NO production but also by dietary NO x and metabolism (8,20,53). The formation of cGMP is also altered by carbon monoxide and atrial natriuretic factor via the activation of guanylate cyclase (27,64,66,71). Therefore, a specific and reliable method of detection is necessary to understand the physiological and pathophysiological role of NO.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of systemic blood NO dynamics by EPR spectroscopy: HbNO as an endogenous index of NO

Kirima¹,

Tsuchiya²,

Séi³

et al. 2003

American Journal of Physiology-Heart and Circulatory Physiology

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.-The measurement of hemoglobin-nitric oxide (NO) adduct (HbNO) in whole blood by the electron paramagnetic resonance (EPR) method seems relevant for the assessment of systemic NO levels. However, ceruloplasmin and unknown radical species overlap the same magnetic field as that of HbNO. To reveal the EPR spectrum of HbNO, we then introduced the EPR signal subtraction method, which is based on the computer-assisted subtraction of the digitized EPR spectrum of HbNO-depleted blood from that of sample blood using the software. Rats were treated with N -nitro-Larginine methyl ester (L-NAME; 120 mg ⅐ kg Ϫ1 ⅐ day Ϫ1 ) for 1 wk to obtain HbNO-depleted blood. When this method was applied to the analysis of untreated fresh whole blood, the five-coordinate state of HbNO was observed. HbNO concentration in pentobarbital-anesthetized rats was augmented (change in [HbNO] ϭ 1.6-5.5 M) by infusion of L-arginine (0.2-0.6 g/kg) but not D-arginine. Using this method, we attempted to evaluate the effects of temocapril on HbNO dynamics in an L-NAME-induced rat endothelial dysfunction model. The oral administration of L-NAME for 2 wk induced a serious hypertension, and the HbNO concentration was reduced (change in [HbNO] ϭ 5.7 M). Coadministration of temocapril dose dependently improved both changes in blood pressure and the systemic HbNO concentration. In this study, we succeeded in measuring the blood HbNO level as an index of NO by the EPR HbNO signal subtraction method. We also demonstrated that temocapril improves abnormalities of NO dynamics in L-NAME-induced endothelial dysfunction rats using the EPR HbNO signal subtraction method. nitric oxide; hemoglobin-nitric oxide adduct; electron paramagnetic resonance; temocapril NITRIC OXIDE (NO), a free radical molecule, has numerous roles in various physiological functions, such as the regulation of the cardiovascular, immune, and nervous systems. An imbalance of NO results in many diseases such as hypertension, atherosclerosis, septic and hemorrhage shock, ischemia-reperfusion injury, diabetes, and neurodegenerative disorders (18, 40,70).Because of increasing interest in the effect of NO, an accurate method is required for the measurement of this radical. Several methods for the quantitation of NO such as chemiluminescence (46), methemoglobin formation (23), and electron paramagnetic resonance (EPR) spectroscopy of nitrosyl-metal complexes (15) have been developed (3). The Griess and cGMP methods have been widely used to measure NO production in vivo (35, 62) and in vitro (34, 61), but these values do not always reflect the concentration of NO, because of their poor specificities for blood NO. EPR spectroscopy is a technique used for the detection and measurement of free radical species. EPR methods using endogenous and exogenous spin-trapping agents, which stabilize NO as a polyatomic spin adduct, have been developed (1,4,29, 41,72).In the circulation, NO exists as relatively stable hemoglobin (Hb)-NO adduct (HbNO) (48), which means that the amount of HbNO may reflect the blood NO ...

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Carbon Monoxide Enhances Human Neutrophil Migration in a Cyclic GMP-dependent Way

Cited by 42 publications

References 0 publications

cGMP-dependent Protein Kinase Iβ Regulates Breast Cancer Cell Migration and Invasion via a Novel Interaction with the Actin/Myosin-associated Protein Caldesmon

cGMP-dependent Protein Kinase Iβ Regulates Breast Cancer Cell Migration and Invasion via a Novel Interaction with the Actin/Myosin-associated Protein Caldesmon

Heme Oxygenase-1 Promoter Polymorphism Protects Liver Allograft

Evaluation of systemic blood NO dynamics by EPR spectroscopy: HbNO as an endogenous index of NO

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