Carbon Monoxide-Releasing Micelles for Immunotherapy

Hasegawa, Urara; Vlies, André J. van der; Simeoni, Eleonora; Wandrey, Christine; Hubbell, Jeffrey A.

doi:10.1021/ja1075025

Cited by 205 publications

(245 citation statements)

References 43 publications

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“…15. [203,587] 357 [357] 358 [582] 356 [239] 353 [222] 352 [222] 354 [236] 355 [427] 359 [170] 360 [533] 368 [292] 366 [486] 367 [423] 369 [58] 370 [383] 371 [245,246] 372 [464] AMPS Ϫ ϩ ϩ Ϫ ϩ ϩ Ϫ 363 [245,246] 361 [328] 362 [328] 364 [289] 365 [383] Ϫ Fig. 8.…”

Section: Monomers With Reactive Functionalitymentioning

confidence: 99%

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Living Radical Polymerization by the RAFT Process – A Third Update

2012

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This paper provides a third update to the review of reversible deactivation radical polymerization (RDRP) achieved with thiocarbonylthio compounds (ZC(¼S)SR) by a mechanism of reversible addition-fragmentation chain transfer ( Chem. 2009Chem. , 62, 1402. This review cites over 700 publications that appeared during the period mid 2009 to early 2012 covering various aspects of RAFT polymerization which include reagent synthesis and properties, kinetics and mechanism of polymerization, novel polymer syntheses, and a diverse range of applications. This period has witnessed further significant developments, particularly in the areas of novel RAFT agents, techniques for end-group transformation, the production of micro/nanoparticles and modified surfaces, and biopolymer conjugates both for therapeutic and diagnostic applications.

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Section: Monomers With Reactive Functionalitymentioning

confidence: 99%

“…[114] 249 PEO macro-RAFT agent [533] 360/BAm [533] 250 N S S A* MMA [534] MA [534] 364 [289] (NES) [290] (BES) [290] 452 [535] NIPAm/NVPI [517] MMA-b-PS [534] MA-b-PS [534] 364-b-387 [289] 251…”

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confidence: 99%

Living Radical Polymerization by the RAFT Process – A Third Update

2012

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“…[ 57] When the solution was irradiated with visible light for 1, 5, and 10 minutes,0 .23 AE 0.03, 0.58 AE 0.08, and 0.70 AE 0.08 equivalents,r espectively,o f CO per Mn were captured by deoxy-Mb.T he amount of CO released without light irradiation was very low (Figure 3a). Thehalf-life (t 1/2 )for CO release from 1 under light exposure was 2.5 AE 0.2 minutes (Figure 3b).…”

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confidence: 99%

“…Intracellular CO release from 1 was confirmed by confocal microscopy with aC Op robe-1 (COP-1). [57] HEK293/kB-Fluc cells containing 1 ([Mn] = 50 mm,i n0 .1m sodium phosphate (pH 7.0), 0.15 m NaCl) were exposed to 456 nm light for 10 minutes to release CO in the cells ( Figure S6). Green fluorescence was observed which is derived from cellular COP-1 as ar esult of the reaction with intracellular CO from 1 ( Figure S6b).…”

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confidence: 99%

A Photoactive Carbon‐Monoxide‐Releasing Protein Cage for Dose‐Regulated Delivery in Living Cells

et al. 2015

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Protein cages can serve as bioinorganic molecular templates for functionalizing metal compounds to regulate cellular signaling. We succeeded in developing a photoactive CO-releasing system by constructing a composite of ferritin (Fr) containing manganese-carbonyl complexes. When Arg52 adjacent to Cys48 of Fr is replaced with Cys, the Fr mutant stabilizes the retention of 48 Mn-carbonyl moieties, which can release the CO ligands under light irradiation, although wild-type Fr retains very few Mn moieties. The amount of released CO is regulated by the extent of irradiation. This could reveal an optimized dose for cooperatively activating the nuclear factor κB (NF-κB) in mammalian cells and the tumor necrosis factor α (TNF-α). These results suggest that construction of a CO-releasing protein cage will advance of research in CO biology.

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“…The CO release under dry conditions was performed in a desiccator and was monitored with a PAC7000 electrochemical CO detector (Drägerwerk AG & Co. KGaA, Lübeck, Germany) (20). In the desiccator, an open quartz cuvette containing a small piece of CORM-1-PLA20 was placed.…”

Section: Methodsmentioning

confidence: 99%

Bactericidal Effect of a Photoresponsive Carbon Monoxide-Releasing Nonwoven against Staphylococcus aureus Biofilms

Klinger-Strobel

Gläser

Makarewicz

et al. 2016

Antimicrob Agents Chemother

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Staphylococcus aureus is a leading pathogen in skin and skin structure infections, including surgical and traumatic infections that are associated with biofilm formation. Because biofilm formation is accompanied by high phenotypic resistance of the embedded bacteria, they are almost impossible to eradicate by conventional antibiotics. Therefore, alternative therapeutic strategies are of high interest. We generated nanostructured hybrid nonwovens via the electrospinning of a photoresponsive carbon monoxide (CO)-releasing molecule [CORM-1, Mn 2 (CO) 10 ] and the polymer polylactide. This nonwoven showed a CO-induced antimicrobial activity that was sufficient to reduce the biofilm-embedded bacteria by 70% after photostimulation at 405 nm. The released CO increased the concentration of reactive oxygen species (ROS) in the biofilms, suggesting that in addition to inhibiting the electron transport chain, ROS might play a role in the antimicrobial activity of CORMs on S. aureus. The nonwoven showed increased cytotoxicity on eukaryotic cells after longer exposure, most probably due to the released lactic acid, that might be acceptable for local and short-time treatments. Therefore, CO-releasing nonwovens might be a promising local antimicrobial therapy against biofilm-associated skin wound infections. Staphylococcus aureus, both methicillin-resistant S. aureus (MRSA) and methicillin-susceptible S. aureus (MSSA), is the leading pathogen in skin and skin structure infections (SSSIs), including surgical and traumatic infections (1, 2). S. aureus has developed a highly effective mechanism to invade tissues and evade the immune system by biofilm formation and intracellular persistence. This can result in chronic and recurring SSSI, particularly in patients with comorbidities such as diabetes mellitus (e.g., diabetic foot syndrome). Noneradicable S. aureus SSSI can be the focus of S. aureus bacteremia, which has a mortality of up to 30% (3). In the United States, community-acquired MRSA has emerged as one of the leading causes of community-acquired SSSI (2).Biofilms are sessile matrix-embedded microbial communities that colonize nearly all artificial and natural surfaces. They are thought to be present in more than 80% of all nosocomial bacterial infections (4). Compared to their planktonic counterparts, bacteria embedded in a biofilm benefit from the protective properties of the matrix, conveying up to 1,000-fold-higher resistance to antibiotics (5). The enhanced phenotypic resistance (correctly termed tolerance) of biofilms is caused mainly by decreased growth rates and metabolically inactive persister cells (a protected phenotypic state) (6). Most antibiotics inhibit metabolic processes in the cell, such as replication (e.g., fluoroquinolones), transcription (e.g., rifampin) or translation (e.g., aminoglycosides and macrolides), and therefore they become ineffective in these persister cells. Altered microenvironments (7) and enzymes hydrolyzing or modifying the antimicrobials that are enriched within the matrix are additio...

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Carbon Monoxide-Releasing Micelles for Immunotherapy

Cited by 205 publications

References 43 publications

Living Radical Polymerization by the RAFT Process – A Third Update

Living Radical Polymerization by the RAFT Process – A Third Update

A Photoactive Carbon‐Monoxide‐Releasing Protein Cage for Dose‐Regulated Delivery in Living Cells

Bactericidal Effect of a Photoresponsive Carbon Monoxide-Releasing Nonwoven against Staphylococcus aureus Biofilms

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