Carbon Nanofibers and Carbon Nanotubes Sensitize Prostate and Bladder Cancer Cells to Platinum-Based Chemotherapeutics

Ringel, Jessica; Erdmann, Kati; Hampel, Silke; Kraemer, Kai; Maier, Diana; Arlt, Marcus; Kunze, Doreen; Wirth, Manfred P.; Fuessel, Susanne

doi:10.1166/jbn.2014.1758

Cited by 32 publications

(38 citation statements)

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“…In contrast, combinations with CNTs led to less pronounced cellular effects, which were of an additive nature. These results are similar to our previous findings for combination of CNFs–CNTs (50 µg/mL) with CDDP and CP (Table 3) [27–28]. In detail, almost all investigated chemotherapeutics led to a synergistically enhanced inhibition of cellular viability in combination with carbon nanomaterials, particularly with CNFs (Table 3).…”

Section: Discussionsupporting

confidence: 91%

“…In combination with at least two or more independent test systems the WST-1 assay can be recommended for evaluating the cellular effects of carbon nanomaterials, because no interference between the water-soluble formazan dye and CNTs has been detected [32]. As reported previously, both CNFs and CNTs impaired cellular viability only marginally in addition to low to moderate effects on cellular proliferation and clonogenic survival [28]. Compared to untreated control cells, both carbon nanomaterials (1–200 µg/mL) exhibited a significant inhibition of cellular viability only in concentrations above 25 µg/mL with CNFs being more detrimental (Supporting Information File 1, Table S1).…”

Section: Resultsmentioning

confidence: 99%

“…Assessment of cellular viability was conducted 72 h after end of treatment. For further details please see the Experimental section and the previous study [28]. b For dose-response curves of chemotherapeutics see Supporting Information File 1: Figure S1.…”

Section: Resultsmentioning

confidence: 99%

“…For DTX and MCC, the cells were treated for 22 h with the carbon nanomaterials and then the chemotherapeutics were added for another 2 h. For CDDP and CP, the cells were treated simultaneously with the chemotherapeutics and the carbon nanomaterials for 24 h. Assessment of cellular function was conducted 72 h after end of treatment. For further details please see the Experimental section and the previous studies [27–28]. b n -Fold increase of expected effect is calculated as the ratio of measured effect to expected effect.…”

Section: Discussionmentioning

confidence: 99%

“…In our previous studies, CNTs and CNFs sensitized prostate and bladder cancer cells to the platinum-based chemotherapeutics carboplatin (CP) and cisplatin (CDDP) via an enhanced inhibition of short- and long-term proliferation as well as by an increased induction of apoptosis [27–28]. In order to further verify this concept and to avoid a bias that might be caused by the structurally similar platinum-based chemotherapeutics, we investigated other chemotherapeutics relevant for urological cancers, namely DTX and mitomycin C (MMC), regarding their cytotoxic effects when applied in combination with carbon nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

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Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation

Erdmann¹,

Ringel²,

Hampel³

et al. 2017

Beilstein J. Nanotechnol.

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We have previously shown that carbon nanofibers (CNFs) and carbon nanotubes (CNTs) can sensitize prostate cancer (PCa) cells to platinum-based chemotherapeutics. In order to further verify this concept and to avoid a bias, the present study investigates the chemosensitizing potential of CNFs and CNTs to the conventional chemotherapeutics docetaxel (DTX) and mitomycin C (MMC), which have different molecular structures and mechanisms of action than platinum-based chemotherapeutics. DU-145 PCa cells were treated with DTX and MMC alone or in combination with the carbon nanomaterials. The impact of the monotreatments and the combinatory treatments on cellular function was then systematically analyzed by using different experimental approaches (viability, short-term and long-term proliferation, cell death rate). DTX and MMC alone reduced the viability of PCa cells to 94% and 68%, respectively, whereas a combined treatment with CNFs led to less than 30% remaining viable cells. Up to 17- and 7-fold higher DTX and MMC concentrations were needed in order to evoke a similar inhibition of viability as mediated by the combinatory treatments. In contrast, the dose of platinum-based chemotherapeutics could only be reduced by up to 3-fold by combination with carbon nanomaterials. Furthermore, combinatory treatments with CNFs led mostly to an additive inhibition of short- and long-term proliferation compared to the individual treatments. Also, higher cell death rates were observed in combinatory treatments than in monotreatments, e.g., a combination of MMC and CNFs more than doubled the cell death rate mediated by apoptosis. Combinations with CNTs showed a similar, but less pronounced impact on cellular functions. In summary, carbon nanomaterials in combination with DTX and MMC evoked additive to partly synergistic anti-tumor effects. CNFs and CNTs possess the ability to sensitize cancer cells to a wide range of structurally diverse chemotherapeutics and thus represent an interesting option for the development of multimodal cancer therapies. Co-administration of chemotherapeutics with carbon nanomaterials could result in a reduction of the chemotherapeutic dosage and thus limit systemic side effects.

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Section: Discussionsupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation

Erdmann¹,

Ringel²,

Hampel³

et al. 2017

Beilstein J. Nanotechnol.

Self Cite

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Carbon Nanotubes in Cancer Diagnosis and Treatment: Current Trends and Future Perspectives

Kumar,

Ansari,

Basu

et al. 2024

Advanced Therapeutics

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Carbon nanotubes (CNTs) have emerged as revolutionary tools, offering innovative approaches to both diagnostic and therapeutic challenges spanning various cancer types. CNTs serve as imaging agents for early detection through MRI, CT, and fluorescence. They provide precise results with biosensors detecting cancer biomarkers for rapid diagnosis. In cancer therapy, CNTs excel in targeted drug delivery, enhancing bioavailability and minimizing off‐target effects. Their prowess in photothermal therapy selectively eradicates cancer cells. Despite challenges, ongoing research highlights CNT's transformative potential, promising enhanced precision, efficacy, and personalized cancer care across diverse malignancies in future clinical practice.

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Development of novel radiochemotherapy approaches targeting prostate tumor progenitor cells using nanohybrids

Nava

Cojoc

Peitzsch

et al. 2015

Intl Journal of Cancer

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Many tumors including prostate cancer are maintained by cancer stem cells (CSCs), which might cause tumor relapse if not eradicated during the course of treatment. Specific targeting or radiosensitization of CSCs bear promise to improve tumor curability by synergistic effects in combination with radiotherapy. Carbon nanotubes (CNTs) can be used as promising drug delivery systems for anticancer drugs such as the flavonoid catechin. Catechin is an extensively studied active ingredient of the different plants, including green tea, and it is widely recognized as co-adjuvant in cancer therapy. Here we describe the synthesis of biocompatible, catechin-loaded and gelatin-conjugated CNTs (Gel_CT_CNTs) with anticancer properties and demonstrate their potential for the eradication of prostate CSCs in combination with X-ray irradiation. Gel_CT_CNTs showed a significant enhancement of in vitro anticancer activity as compared to catechin alone. Moreover, treatment of prostate cancer cells with Gel_CT_CNT nanohybrids inhibited the tumorigenic cell population defined by a high aldehyde dehydrogenase (ALDH) activity. A combination of X-ray irradiation and treatment with Gel_CT_CNTs caused a decrease in the protein level of stem cell-related transcription factors and regulators including Nanog, Oct4 and b-catenin and led to an increase of cancer cell radiosensitivity as demonstrated by clonogenic and spherogenic cell survival assays. Taken together, our results suggest that a combination of irradiation and Gel_CT_CNTs can be potentially used for the radiosensitization and eradication of prostate CSC populations.In Europe, prostate cancer is the third most common cause of cancer and among the leading causes of cancer-related deaths in men. Radiotherapy is one of the key treatments for prostate cancer along with surgery, chemotherapy and hormone therapy.1 A significant proportion of men is diagnosed with curable localized prostate cancer. However, successful treatment of the patients by radiotherapy depends on tumor radiosensitivity and the tolerance of the surrounding normal

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Carbon Nanofibers and Carbon Nanotubes Sensitize Prostate and Bladder Cancer Cells to Platinum-Based Chemotherapeutics

Cited by 32 publications

References 0 publications

Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation

Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation

Carbon Nanotubes in Cancer Diagnosis and Treatment: Current Trends and Future Perspectives

Development of novel radiochemotherapy approaches targeting prostate tumor progenitor cells using nanohybrids

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