Carbon nanomaterial as platform for electrochemical genosensor: A system for the diagnosis of the hepatitis C in real sample

Oliveira, Danielle Alves de; Silva, Jussara V.; Flauzino, José M.R.; Sousa, Heliane S.; Castro, Ana Cristina Honorato de; Moço, Anna C.R.; Soares, Márcia Maria Costa Nunes; Madurro, João M.; Brito‐Madurro, Ana G.

doi:10.1016/j.jelechem.2019.04.045

Cited by 26 publications

(14 citation statements)

References 38 publications

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“…Thus, there is a demand for biosensor development that can produce a quantitative signal selective for specific viral entities ( Chowdhury et al, 2019 ; Grubaugh et al, 2019 ; Jacob et al, 2020 ). The conventional diagnostic tools for antigen detection include chemiluminescence ( Meng et al, 2020 ; Wang et al, 2019 ; Yang et al, 2020 ; Zhang et al, 2020 ), ELISA ( Arce et al, 2019 ; Shrivastava et al, 2019 ), and PCR ( Maartens et al, 2020 ; Mishra et al, 2019 ; Oliveira et al, 2019a ; Pathirana et al, 2019 ). However, the latter methods require highly skilled personnel and suitable laboratory environment.…”

Section: Methods Of Detection Of Viral Pathogens Of Humansmentioning

confidence: 99%

“…Development of an electrochemical genosensor for the diagnosis of HCV in real samples was reported by Oliveira et al based on a gold electrode modified with GO and ethylenediamine (GO-ETD) ( Oliveira et al, 2019b ). The modification was useful to probe DNA immobilization following interaction with the target DNA HCV through hybridization.…”

Section: Graphene-based Materials In Virus Sensingmentioning

confidence: 99%

“…The LOD was 1:483 (v/v) or 1.36 nmol L −1 of RNA. Thus, the selective and specific diagnosis of hepatitis C could be achieved by this genosensor ( Oliveira et al, 2019b ).…”

Section: Graphene-based Materials In Virus Sensingmentioning

confidence: 99%

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Human virus detection with graphene-based materials

Vermisoglou

Panáček

Jayaramulu

et al. 2020

Biosensors and Bioelectronics

161

117

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Our recent experience of the COVID-19 pandemic has highlighted the importance of easy-to-use, quick, cheap, sensitive and selective detection of virus pathogens for the efficient monitoring and treatment of virus diseases. Early detection of viruses provides essential information about possible efficient and targeted treatments, prolongs the therapeutic window and hence reduces morbidity. Graphene is a lightweight, chemically stable and conductive material that can be successfully utilized for the detection of various virus strains. The sensitivity and selectivity of graphene can be enhanced by its functionalization or combination with other materials. Introducing suitable functional groups and/or counterparts in the hybrid structure enables tuning of the optical and electrical properties, which is particularly attractive for rapid and easy-to-use virus detection. In this review, we cover all the different types of graphene-based sensors available for virus detection, including, e.g., photoluminescence and colorimetric sensors, and surface plasmon resonance biosensors. Various strategies of electrochemical detection of viruses based on, e.g., DNA hybridization or antigen-antibody interactions, are also discussed. We summarize the current state-of-the-art applications of graphene-based systems for sensing a variety of viruses, e.g., SARS-CoV-2, influenza, dengue fever, hepatitis C virus, HIV, rotavirus and Zika virus. General principles, mechanisms of action, advantages and drawbacks are presented to provide useful information for the further development and construction of advanced virus biosensors. We highlight that the unique and tunable physicochemical properties of graphene-based nanomaterials make them ideal candidates for engineering and miniaturization of biosensors.

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Section: Methods Of Detection Of Viral Pathogens Of Humansmentioning

confidence: 99%

Section: Graphene-based Materials In Virus Sensingmentioning

confidence: 99%

See 1 more Smart Citation

Human virus detection with graphene-based materials

Vermisoglou

Panáček

Jayaramulu

et al. 2020

Biosensors and Bioelectronics

161

117

View full text Add to dashboard Cite

show abstract

“…Authors argued that the speed, sensitivity, and selectivity of this first-of-its-kind graphene-enabled Zika biosensor make it an ideal candidate for development as a medical diagnostic test. In other study, Oliveira et al [49] reported the development of an electrochemical genosensor for the diagnosis of Hepatitis C virus (HCV) in real samples based on a gold electrode modified with GO and ethylenediamine. The modification was useful to probe DNA immobilization following interaction with the target DNA HCV through hybridization.…”

Section: Graphene Biosensors For Virus Detectionmentioning

confidence: 99%

Viewpoint: How the Graphene Could Help to Decrease SARS-CoV-2 Spread?

Ruíz-Santoyo

Romero-Toledo

Andrade-Espinoza

et al. 2021

Period. Polytech. Chem. Eng.

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The COVID-19 (Coronavirus Disease 2019), caused by the SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) began in December 2019 in Wuhan, China. Until February 2021, there are 110 million of infected people, 60 million have recovered and approximately 2.5 million have passed away worldwide according to WHO. The coronavirus pandemic is evolving very rapidly and represents a risk for health care workers and society in general. Moreover, pandemic has tested the limits of health systems by raising questions about forms of prevention, management of infections with conventional therapies and the use of diagnostic tools. In this article we discussed the possible role of the nanostructured-graphene based materials as aid tools for preventing the spread and infection of SARS-CoV-2. In this regard, nanotechnology could take part in the fight against the spread of future diseases caused by deadly viruses. However, its use should be well founded in terms of biocompatibility. Therefore, we have proposed an approach based on graphene nanomaterials as possible allies for the fight against the COVID-19 spread based on the physicochemical features that present these novel nanomaterials.

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“…In that sense graphene nanoribbons (GNRs) are an excellent choice for the design of nanostructured genosensors, due to their electrical properties and remarkably high length‐to‐diameter and surface‐to‐volume ratios 11 . As carbon nanostructures they can be multifunctionalized to be used as suitable scaffolds to tether molecular probes onto them 12 . Graphene is a two‐dimensional biocompatible semiconducting nanomaterial.…”

Section: Introductionmentioning

confidence: 99%

Impedimetric genosensor based on graphene nanoribbons for detection and identification of oncogenic types of human papillomavirus

Lucena

Frías

Lucena‐Silva

et al. 2021

J of Chemical Tech & Biotech

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BACKGROUND Human papillomavirus (HPV) is a large group of viruses that infect skin and mucosa causing warts and epithelial tumors known as papillomas. Although HPV can be contracted during sexual intercourse including vaginal, anal and oral sex, skin‐to‐skin contact is sufficient to cause infection. Asymptomatic patients can have the virus and pass it on without knowing it. Due to the scarcity of rapid and inexpensive methods available to identify HPV serotypes, herein we report an electrochemical genosensor as an alternative to current molecular and serological HPV identification methods. Graphene nanoribbons were used as transducers to immobilize MY11 DNA probe, a degenerate probe generally employed in polymerase chain reaction amplification of a broad spectrum of HPV DNA types. RESULTS Cyclic voltammetry, electrochemical impedance spectroscopy and atomic force microscopy were used to characterize the construction of the platform and the biodetection process. Our results suggest that oncogenic subtypes of HPV are quickly detected and identified in the picogram range. Impedimetric responses grouped for known genotypically related HPV 51, 53 and 66 subtypes related to anogenital lesions. Additionally, our genosensor was able to successfully detect HPV types 16 and 18 in cervical specimens (cDNA samples) at concentrations of 1 amol L−1, 100 amol L−1, 100 fmol L−1, 50 pmol L−1 and 100 pmol L−1 with a detection limit of 3.5 and 1.2 amol L−1 for HPV 16 and HPV 18, respectively. CONCLUSIONS The proposed genosensor platform can be considered a new tool for detecting and identifying HPV genotypes. © 2021 Society of Chemical Industry (SCI).

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Carbon nanomaterial as platform for electrochemical genosensor: A system for the diagnosis of the hepatitis C in real sample

Cited by 26 publications

References 38 publications

Human virus detection with graphene-based materials

Human virus detection with graphene-based materials

Viewpoint: How the Graphene Could Help to Decrease SARS-CoV-2 Spread?

Impedimetric genosensor based on graphene nanoribbons for detection and identification of oncogenic types of human papillomavirus

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