Carbon nanomaterials-based electrochemical aptasensor for point-of-care diagnostics of cancer biomarkers

Parihar, Arpana; Choudhary, Nishant Kumar; Sharma, Palak; Khan, Raju

doi:10.1016/j.mtchem.2023.101499

Cited by 31 publications

(17 citation statements)

References 139 publications

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“…The sensing platform included a second MTF electrode capped with a control aptamer, which did not react with the target, to provide a baseline and correction to drifts due to interferences. 163 Finally, field-effect transistor (FET) biosensors based on nanomaterials such as silicon nanowires 164 and graphene 165 have the potential of rapid and label-free detection of a wide range of biomarkers. Indeed, FET biosensors detect changes in the charge distribution at the surface of the transistor when biomolecules bind to the biorecognition elements on the gate electrode or the transistor channel.…”

Section: Further Applications In Biosensingmentioning

confidence: 99%

“…Finally, field-effect transistor (FET) biosensors based on nanomaterials such as silicon nanowires and graphene have the potential of rapid and label-free detection of a wide range of biomarkers. Indeed, FET biosensors detect changes in the charge distribution at the surface of the transistor when biomolecules bind to the biorecognition elements on the gate electrode or the transistor channel .…”

Section: Further Applications In Biosensingmentioning

confidence: 99%

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Ordered Mesoporous Electrodes for Sensing Applications

et al. 2023

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Electrochemical sensors have become increasingly relevant in fields such as medicine, environmental monitoring, and industrial process control. Selectivity, specificity, sensitivity, signal reproducibility, and robustness are among the most important challenges for their development, especially when the target compound is present in low concentrations or in complex analytical matrices. In this context, electrode modification with Mesoporous Thin Films (MTFs) has aroused great interest in the past years. MTFs present high surface area, uniform pore distribution, and tunable pore size. Furthermore, they offer a wide variety of electrochemical signal modulation possibilities through molecular sieving, electrostatic or steric exclusion, and preconcentration effects which are due to mesopore confinement and surface functionalization. In order to fully exploit these advantages, it is central to develop reproducible routes for sensitive, selective, and robust MTF-modified electrodes. In addition, it is necessary to understand the complex mass and charge transport processes that take place through the film (particularly in the mesopores, pore surfaces, and interfaces) and on the electrode in order to design future intelligent and adaptive sensors. We present here an overview of MTFs applied to electrochemical sensing, in which we address their fabrication methods and the transport processes that are critical to the electrode response. We also summarize the current applications in biosensing and electroanalysis, as well as the challenges and opportunities brought by integrating MTF synthesis with electrode microfabrication, which is critical when moving from laboratory work to in situ sensing in the field of interest.

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Section: Further Applications In Biosensingmentioning

confidence: 99%

Section: Further Applications In Biosensingmentioning

confidence: 99%

Ordered Mesoporous Electrodes for Sensing Applications

et al. 2023

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“…In this context, compared to other user-friendly/traditional methods, electrochemical methods have been considered more favorable techniques due to their straightforward operation, cost-effectiveness, high sensitivity, and selectivity. 14 immunosensors, aptamer-based aptasensors have gained considerable interest for the fabrication of electrochemical biosensors due to their low cost, ease of synthesis, robustness, and high selectivity for the detection of biomarkers. 15,21 For instance, Wang et al 21 used anti-EGFR aptamers as the biorecognition element for the fabrication of an electrochemical paper-based aptasensor for label-free and ultrasensitive detection of EGFR.…”

Section: Introductionmentioning

confidence: 99%

“…27,28 Recent years have seen a rise in interest in electrode surface modifications using GO-based materials, which provide affordable electrochemical sensors with large surface areas, good functionalities, and excellent biocompatibility. 14 However, during electrode modification, the GO sheets clump together on the electrode surfaces, producing unwanted graphitelike structures that interfere with the sensor displays.…”

Section: Introductionmentioning

confidence: 99%

Yttrium Functionalized Reduced Graphene Oxide Nanocomposite-Based Aptasensor for Ultrasensitive Detection of a Breast Cancer Biomarker

Parihar,

Khan

2023

ACS Appl. Nano Mater.

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Higher cancer mortality rate can be prevented by the early detection of cancer-associated biomarkers and appropriate therapeutic intervention. Recently, electrochemical biosensors have drawn a great deal of interest because of their extremely high sensitivity, selectivity, and affordability in early cancer detection. Herein, we fabricated an aptasensor for the inexpensive and label-free detection of the epidermal growth factor receptor (EGFR) antigen, a biomarker linked to breast cancer. The reduced graphene oxide–yttrium nanocomposite was synthesized and characterized using FTIR, XRD, TEM, SEM, Raman spectroscopy, and UV–vis spectroscopy, which confirmed the synthesis of the nanocomposite. The synthesized material was used for the fabrication of electrochemical aptasensor for the detection of epidermal growth factor receptor antigen. The developed sensor (Y2O3–rGO/Apt/BSA) showed a wide linear detection range (10 fg mL–1 to 100 ng mL–1), a low detection limit of 0.251 fg mL–1, and an excellent sensitivity of 51.96 μA fM–1·cm–2. The aptasensor also provides a straightforward and quick approach to EGFR antigen detection in biological serum samples. The simple and facile synthesis method reported in this work led to the production of a high-purity, water-dispersible yttrium functionalized reduced graphene oxide nanocomposite for cost-effective, faster, and ultrasensitive detection of breast cancer biomarkers using electrochemical aptasensors.

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“…The development of biosensors using novel materials such as reduced graphene oxide and carbon nanotubes, etc. may lead to further improvements in sensitivity and selectivity. , Herein, both CdS NPs and CdS/rGO nanocomposites were synthesized using bacterial biomass, and their electrochemical, antimicrobial, and photocatalytic properties were evaluated. The outcome of this research showed that the green synthesized CdS NPs and CdS-rGO displayed good electrochemical, antimicrobial, and photocatalytic properties.…”

Section: Introductionmentioning

confidence: 99%

Green Synthesis of CdS and CdS/rGO Nanocomposites: Evaluation of Electrochemical, Antimicrobial, and Photocatalytic Properties

Parihar,

Sharma,

Choudhary

et al. 2023

ACS Appl. Bio Mater.

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The green approach has been employed for the synthesis of various types of nanomaterials including metal nanoparticles, metal oxides, and carbon-based nanomaterials. These processes involve natural sources that contain bioactive compounds that act as reducing, stabilizing, and capping agents for the formation and stabilization of nanomaterials. This study reports the green synthesis of CdS and CdS/rGO nanocomposites using Lactobacillus bacteria. The UV–visible spectrophotometer, field emission scanning electron microscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy, Raman spectroscopy, and Fourier transform infrared spectroscopy spectra confirm the synthesis of the nanocomposite. The electrochemical characterization using cyclic voltammetry, differential pulse voltammetry, and EIS revealed that the CdS/rGO nanocomposites showed a higher electron transfer rate compared with CdS nanoparticles, indicating the potential of the nanocomposites for biosensing applications. The zone of inhibition revealed significant antimicrobial activity against Escherichia coli and Staphylococcus aureus for both CdS nanoparticles and CdS/rGO nanocomposites. Additionally, CdS/rGO nanoparticles exhibited high photocatalytic activity for the degradation of methylene blue dye. Overall, this study demonstrates that the synthesized CdS and CdS/rGO nanocomposites have good electrochemical properties, photocatalytic, and antimicrobial activity and, therefore, can be employed for various applications such as biosensing, photocatalysis, and antimicrobial activity.

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Carbon nanomaterials-based electrochemical aptasensor for point-of-care diagnostics of cancer biomarkers

Cited by 31 publications

References 139 publications

Ordered Mesoporous Electrodes for Sensing Applications

Ordered Mesoporous Electrodes for Sensing Applications

Yttrium Functionalized Reduced Graphene Oxide Nanocomposite-Based Aptasensor for Ultrasensitive Detection of a Breast Cancer Biomarker

Green Synthesis of CdS and CdS/rGO Nanocomposites: Evaluation of Electrochemical, Antimicrobial, and Photocatalytic Properties

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