Carbon nanoparticle induced cytotoxicity in human mesenchymal stem cells through upregulation of TNF3, NFKBIA and BCL2L1 genes

Periasamy, Vaiyapuri Subbarayan; Athinarayanan, Jegan; Alfawaz, Mohammed; Alshatwi, Ali A.

doi:10.1016/j.chemosphere.2015.08.018

Cited by 34 publications

(24 citation statements)

References 54 publications

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“…Other studies have shown that carbon-based nanoparticles, either commercially available or formed in bread-making process, have moderate toxic effects on self-renewal of MSCs. However, these studies did not investigate nanoparticle effects on osteogenic differentiation [ 42 , 43 ]. Studies on the purple sea urchin ( Paracentrotus lividus ) have demonstrated that the exposure of sea urchin sperm to CB impaired primary mesenchymal cell migration and anomalous arrangements of the skeletal rod [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Carbon black suppresses the osteogenesis of mesenchymal stem cells: the role of mitochondria

Shen

Qin

et al. 2018

Part Fibre Toxicol

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BackgroundThe rapid increase in carbon black poses threats to human health. We evaluated the effect of CB (Printex 90) on the osteogenesis of bone-marrow-derived mesenchymal stem cells (MSCs). Mitochondria play an important role in the osteogenesis of MSCs and are potential targets of nanomaterials, so we studied the role of mitochondria in the CB Printex 90-induced effects on osteogenesis.ResultsLow doses of Printex 90 (3 ng/mL and 30 ng/mL) that did not cause deleterious effects on MSCs’ viability significantly inhibited osteogenesis of MSCs. Printex 90 caused down-regulation of osteoblastic markers, reduced activity of alkaline phosphatase (ALP), and poor mineralization of osteogenically induced MSCs. Cellular ATP production was decreased, mitochondrial respiration was impaired with reduced expression of ATPase, and the mitochondrial membrane was depolarized. The quantity and quality of mitochondria are tightly controlled by mitochondrial biogenesis, mitochondrial dynamics and mitophagy. The transcriptional co-activator and transcription factors for mitochondrial biogenesis, PGC-1α, Nrf1 and TFAM, were suppressed by Printex 90 treatment, suggesting that decreased biogenesis was caused by Printex 90 treatment during osteogenesis. Mitochondrial fusion and fission were significantly inhibited by Printex 90 treatment. PINK1 accumulated in Printex 90-treated cells, and more Parkin was recruited to mitochondria, indicating that mitophagy increased to remove the damaged mitochondria.ConclusionsThis is the first report of the inhibitory effects of CB on the osteogenesis of MSCs and the involvement of mitochondria in CB Printex 90-induced suppression of MSC osteogenesis.Electronic supplementary materialThe online version of this article (10.1186/s12989-018-0253-5) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Carbon black suppresses the osteogenesis of mesenchymal stem cells: the role of mitochondria

Shen

Qin

et al. 2018

Part Fibre Toxicol

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“…Comet tail formation attributed to the DNA breaks or failed DNA repair mechanisms induced by oxidative stress. As supported by FT-IR, XPS, and ʐ-potential results, CD surface bearing a high amount of oxygenated functional groups could lead to the production of ROSs, and as a consequence, the oxidative stress may lead the genomic instability 97,107,108 . A recent study by Zhou et al 90 also indicated that modulating the oxygenated groups, most effectively the number of ketonic carboxyl groups, ROS production by carbon dots can also be controlled, the highest the oxygenated groups on the surface the highest the ROS production capability.…”

Section: Resultsmentioning

confidence: 92%

“…A comparison of the present study and previous literature is provided in Table 2. Most of the listed studies showed that negatively charged CDs with a size lower than 10 nm which can freely enter or interact with the nucleus while there are a couple of examples reporting nucleus targeting by larger sized CDs 47,92,100 , relatively few of them addressing the CD-induced anomalies in cell-cycle 49,108,110,111 . Our results suggest that CDs could have the potential as both drug carriers interacting with the cell-nucleus and therapeutic agents against tumor cells (Scheme 1).…”

Section: Resultsmentioning

confidence: 99%

“…al. 108 Commercial CDs - < 50 nm Cell cytotoxicity Cell Type : hMSCs, human mesenchymal stem cells CNPs moderately reduce cell viability and cause chromatin condensation and DNA fragmentation, disrupt the expression of cell death genes Cell cycle progression of hMSCs was arrested slightly, the number of cells in G0/G1 increased at low concentrations of CNP exposure, cell cycle was arrested in the sub-G0/G1 phase in a dose-dependent manner Kumawat et al 50 Grape seed extract Microwave 50–60 nm Nucleus Imaging, and Photoluminescent Sensing Cell Type : L929, HT-1080, MIA PaCa-2, HeLa, and MG-63 cells The tendency to self-localize themselves into cell nucleus regardless of cell-type. No cytotoxicity and act as an enhancer in cell proliferation in L929 confirmed by in vitro wound scratch assay and cell cycle analysis.…”

Section: Resultsmentioning

confidence: 99%

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DNA-damage and cell cycle arrest initiated anti-cancer potency of super tiny carbon dots on MCF7 cell line

Şimşek

Şüküroğlu

Yetkin

et al. 2020

Sci Rep

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While carbon-based materials have spearheaded numerous breakthroughs in biomedicine, they also have procreated many logical concerns on their overall toxicity. carbon dots (cDs) as a respectively new member have been extensively explored in nucleus directed delivery and bioimaging due to their intrinsic fluorescence properties coupled with their small size and surface properties. Although various in vitro/in vivo studies have shown that CDs are mostly biocompatible, sufficient information is lacking regarding genotoxicity of them and underlying mechanisms. This study aims to analyze the real-time cytotoxicity of super tiny CDs (2.05 ± 0.22 nm) on human breast cancer cells (MCF7) and human primary dermal fibroblast cell cultures (HDFa) by xCELLigence analysis system for further evaluating their genotoxicity and clastogenicity to evaluate the anti-tumor potential of cDs on breast adenocarcinoma. As combined with flow cytometry studies, comet assay and cytokinesisblock micronucleus assay suggest that the CDs can penetrate to the cell nuclei, interact with the genetic material, and explode DNA damage and G0/G1 phase arrest in cancer cells even at very low concentrations (0.025 ppm) which provide a strong foundation for the design of potentially promising cD-based functional nanomaterials for DnA-damage induced treatment in cancer therapy. Functional nanomaterials that can better target cancer cells that will improve prevention and therapy could be accomplished by the combined efforts of nanotoxicologists, cancer biologists and nanobiomaterial scientists focused on toxicology and related cancer therapy. Engineering carbon-based nanomaterials and their applications are among the most dynamic fields in modern advanced materials science and engineering 1-3. To date, various carbon nanomaterials such as carbon nanotubes 4,5 , fullerenes 6 , graphene 7,8 , graphene oxides 9 , carbon diamonds 10,11 , and carbon dots 12,13 have been synthesized and reported by various researchers. Among these, CDs have become particularly interesting because of their unique physical and chemical properties such as thermal and electrical conductivity, high mechanical strength together with their unique optic and fluorescence features 14-16. They have been used in bio-imaging, drug delivery, nucleus targeting, and labeling, photodynamic therapy, optoelectronics, solar cells, photocatalyst design, photodetectors, and many other biological and engineering fields 17-26. Several methods are available for the synthesis of CDs, namely, laser ablation 15 , electro-oxidation 27 or oxidative acid treatment 12 ; while nowadays, less laborsome and cheaper methods such as hydrothermal 28 and thermal synthesis 24,29,30 , microwave-assisted hydrothermal synthesis 31 , ultrasound synthesis 32 , etc. are popularly exercised. CDs can be synthesized either from inorganic materials 19,33 such as graphene 34 , carbon black 35 , and candle soot 12

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“…However, the growing interest in using CNs in the biomedical field has raised significant concerns about their biocompatibility, clearly pointing out that the effects of the nanostructures on the biological environment must be carefully assessed in order to take full advantage of their unique characteristics and to define their safe use. The cytotoxicity and immunological response of different types of CNs have been thoroughly investigated both in vitro and in vivo, disclosing a quite complex landscape [21][22][23][24]. Experimental studies have shown that cell response depends on several factors, such as CN physicochemical properties and geometrical structure, surface functionalization, size distribution, presence of impurities, wettability and dispersibility in aqueous media, as well as on the culture medium and target cell type [25][26][27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Hemocompatibility of Carbon Nanostructures

Fedel

2020

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Carbon nanostructures (CNs), such as carbon nanotubes, fullerenes, carbon dots, nanodiamonds as well as graphene and its derivatives present a tremendous potential for various biomedical applications, ranging from sensing to drug delivery and gene therapy, biomedical imaging and tissue engineering. Since most of these applications encompass blood contact or intravenous injection, hemocompatibility is a critical aspect that must be carefully considered to take advantage of CN exceptional characteristics while allowing their safe use. This review discusses the hemocompatibility of different classes of CNs with the purpose of providing biomaterial scientists with a comprehensive vision of the interactions between CNs and blood components. The various complex mechanisms involved in blood compatibility, including coagulation, hemolysis, as well as the activation of complement, platelets, and leukocytes will be considered. Special attention will be paid to the role of CN size, structure, and surface properties in the formation of the protein corona and in the processes that drive blood response. The aim of this review is to emphasize the importance of hemocompatibility for CNs intended for biomedical applications and to provide some valuable insights for the development of new generation particles with improved performance and safety in the physiological environment.

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Carbon nanoparticle induced cytotoxicity in human mesenchymal stem cells through upregulation of TNF3, NFKBIA and BCL2L1 genes

Cited by 34 publications

References 54 publications

Carbon black suppresses the osteogenesis of mesenchymal stem cells: the role of mitochondria

Carbon black suppresses the osteogenesis of mesenchymal stem cells: the role of mitochondria

DNA-damage and cell cycle arrest initiated anti-cancer potency of super tiny carbon dots on MCF7 cell line

Hemocompatibility of Carbon Nanostructures

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