Carbon nanotube-based aptasensor for sensitive electrochemical detection of whole-cell Salmonella

Hasan, Rakibul; Pulingam, Thiruchelvi; Appaturi, Jimmy Nelson; Zifruddin, Anis Nadyra; Teh, Swe Jyan; Lim, Teck Wei; Ibrahim, Fatimah; Leo, Bey Fen; Thong, Kwai Lin

doi:10.1016/j.ab.2018.06.001

Cited by 95 publications

(28 citation statements)

References 39 publications

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“…Aptamer immobilization at different ionic strength Since the lateral density of aptamer probes is of critical importance for the sensitivity of aptasensors, [43][44][45][46] two lengths of polyA (i.e., 10A and 30A) were employed here in order to control the aptamer lateral density on a planar gold substrate. Based on experience obtained from our previous work, 19 the immobilization of polyA-containing aptamers was carried out by immersing the gold substrate into 1.0 μM of an aptamer solution, in which NaCl was added up to 1.0 M to provide a relatively high ionic strength.…”

Section: Resultsmentioning

confidence: 99%

Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens

2019

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Nucleic acid aptamers have been widely used as synthetic probes for bioanalytical applications. Herein, we carried out a detailed study on the immobilization of a series of aptamers ranging from 37 to 88 bases, which are specific to either Escherichia coli (E. coli) or Staphylococcus aureus (S. aureus), on a planar gold substrate via a polyadenine-mediated immobilization method. The resultant surfaces were characterized by both surface plasmon resonance spectroscopy (SPR) and X-ray photoelectron spectroscopy. The results clearly show that the aptamer solution at a lower ionic strength gives rise to a higher lateral density of the aptamer when compared to that at a higher ionic strength. The SPR aptasensors are then employed for detecting their corresponding bacteria (i.e., E. coli and S. aureus, respectively). The data indicate that the SPR aptasensor with a higher density of aptamer exhibits a better capture of target bacteria.

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Section: Resultsmentioning

confidence: 99%

Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens

2019

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“…Using one of the aptamers (5'-TAT GGC GGC GTC ACC CGA CGG GGA CTT GAC ATT ATG ACA G-3'), they developed an aptamer-based trapping PCR detection method with a detection limit of 1 CFU/mL in rinsate samples 46 . This aptamer was broadly applied in various detection methods based on different aptasensor technologies, with detection limits ranging from 1 to 1000 CFU/mL 47 , 158 - 168 , suggesting that this aptamer is worth to be applied to develop commercial products. In addition, using Vi-antigen as the target, Pathania et al developed a DNA aptamer using a microtiter-based SELEX approach.…”

Section: Potential Utility Of Aptamer Technology For Pathogen Detectionmentioning

confidence: 99%

Oligonucleotide aptamers for pathogen detection and infectious disease control

Wan

Liu

2021

Theranostics

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During an epidemic or pandemic, the primary task is to rapidly develop precise diagnostic approaches and effective therapeutics. Oligonucleotide aptamer-based pathogen detection assays and control therapeutics are promising, as aptamers that specifically recognize and block pathogens can be quickly developed and produced through simple chemical synthesis. This work reviews common aptamer-based diagnostic techniques for communicable diseases and summarizes currently available aptamers that target various pathogens, including the SARS-CoV-2 virus. Moreover, this review discusses how oligonucleotide aptamers might be leveraged to control pathogen propagation and improve host immune system responses. This review offers a comprehensive data source to the further develop aptamer-based diagnostics and therapeutics specific for infectious diseases.

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“…For example, graphene has received great attention via different variants such as Graphene quantum dots (GQDs), reduced graphene oxide (rGO), graphene oxide and graphene composites [146]. The carbon nanotubes (CNTs) (either multiple walls or single wall) and fullerenes, are other increasingly used nanomaterials for biosensors with enhanced performance due to their interesting catalytic, mechanical, and electrical properties [84,147]. Taking the advantages of non-toxicity, biocompatibility, and high chemical and physical stability, the silica nanoparticles (SiNPS) have also been explored [148,149].…”

Section: ) Biosensor For Pathogens Monitoringmentioning

confidence: 99%

Connected Sensors, Innovative Sensor Deployment, and Intelligent Data Analysis for Online Water Quality Monitoring

Manjakkal

Mitra

Pétillot

et al. 2021

IEEE Internet Things J.

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The sensor technology for water quality monitoring (WQM) has improved during recent years. The cost-effective sensorised tools that can autonomously measure the essential physical -chemical and biological (PCB) variables are now readily available and are being deployed on buoys, boats and ships. Yet, there is a disconnect between the data quality, data gathering and data analysis due to the lack of standardized approaches for data collection and processing, spatio-temporal variation of key parameters in water bodies and new contaminants. Such gaps can be bridged with a network of multiparametric sensor systems deployed in water bodies using autonomous vehicles such as marine robots and aerial vehicles to broaden the data coverage in space and time. Further, intelligent algorithms (e. g. artificial intelligence (AI)) could be employed for standardised data analysis and forecasting. This paper presents a comprehensive review of the sensors, deployment and analysis technologies for WQM. A network of networked water bodies could enhance the global data intercomparability and enable WQM at global scale to address global challenges related to food (e.g., aqua/agriculture), drinking water, and health (e.g., water borne diseases).

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Carbon nanotube-based aptasensor for sensitive electrochemical detection of whole-cell Salmonella

Cited by 95 publications

References 39 publications

Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens

Polyadenine-mediated Immobilization of Aptamers on a Gold Substrate for the Direct Detection of Bacterial Pathogens

Oligonucleotide aptamers for pathogen detection and infectious disease control

Connected Sensors, Innovative Sensor Deployment, and Intelligent Data Analysis for Online Water Quality Monitoring

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