Carbon nanotube-based ultrasensitive multiplexing electrochemical immunosensor for cancer biomarkers

Wan, Ying; Deng, Wangping; Su, Yan; Zhu, Xiangwei; Cheng, Peng; Hu, Haiyan; Peng, Hongzhen; Song, Shiping; Fan, Chunhai

doi:10.1016/j.bios.2011.08.033

Cited by 152 publications

(53 citation statements)

References 41 publications

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“…for promoting the signal as well as detection sensitivity in bioanalytical studies. 23 This proposal has been applied to improve the sensitivity in optical biosensors, 24 electrochemical [25][26][27] and pathogen detection. 23 This paper described a biotin-streptavidin-enhanced carbon nanotube amplification strategy for an ultrasensitive determination of PBDEs.…”

Section: Introductionmentioning

confidence: 99%

A Biotin-streptavidin-enhanced Carbon Nanotube Amplification Strategy for an Ultrasensitive Immunodetection of Polybrominated Diphenyl Ethers

Zhang

Zhuang

2017

ANAL. SCI.

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The extensive use of polybrominated diphenyl ethers (PBDEs) has resulted in its increasingly widespread presence. Especially the lower halogenated PBDEs accumulate to a greater degree than the higher halogenated PBDEs in house dust, sewage sludge, pets, or even humans. In the present work, we developed an ultrasensitive biotin-streptavidinenhanced carbon nanotube amplification strategy for the immunodetection of PBDEs, in which single-walled carbon nanotubes were used to immobilize numerous streptavidin. Meanwhile, we used biotin conjugated horseradish peroxidase (B-HRP) and biotin conjugated Goat anti-rabbit (B-IgG) to link the HRP and IgG to CNTs by using a biotin-streptavidin system. The sensitivity of the streptavidin-biotin-IgG-CNTs-HRP bioconjugate was compared with a commercial HRPlabelled IgG by using indirect competitive ELISA. The limit of this proposed ELISA detection (IC10) was 0.0059 ng/mL, showing a 20-time lower detection limit over the commercial one (IC10 = 0.1193 ng/mL). Finally, we applied the assay to the detection of PBDEs in dust samples. The results were consistent with those using GC-ECD, which confirmed that the proposed amplification strategy was accurate and receptive. This proposed biotin-streptavidin-enhanced carbon nanotube amplification strategy would be useful for ultrasensitive immunodetection in environmental studies.

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Section: Introductionmentioning

confidence: 99%

A Biotin-streptavidin-enhanced Carbon Nanotube Amplification Strategy for an Ultrasensitive Immunodetection of Polybrominated Diphenyl Ethers

Zhang

Zhuang

2017

ANAL. SCI.

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“…In immunosensors, this biochemical reaction resulting in the Ab-Ag complex formation ultimately produces a detectable signal by way of the transducer component in the biosensor. Of note, special care must be taken when selecting the assembly conditions of the Abs with the nanomaterials because different Ab molecules have unalike electrostatic properties therefore adjustments must be made in the Ab-nanomaterial conjugate assembly conditions of different Abs even when the same nanomaterials are used [38]. …”

Section: Nano- and Biomaterialsmentioning

confidence: 99%

Nanomaterial-Based Electrochemical Immunosensors for Clinically Significant Biomarkers

2014

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Nanotechnology has played a crucial role in the development of biosensors over the past decade. The development, testing, optimization, and validation of new biosensors has become a highly interdisciplinary effort involving experts in chemistry, biology, physics, engineering, and medicine. The sensitivity, the specificity and the reproducibility of biosensors have improved tremendously as a result of incorporating nanomaterials in their design. In general, nanomaterials-based electrochemical immunosensors amplify the sensitivity by facilitating greater loading of the larger sensing surface with biorecognition molecules as well as improving the electrochemical properties of the transducer. The most common types of nanomaterials and their properties will be described. In addition, the utilization of nanomaterials in immunosensors for biomarker detection will be discussed since these biosensors have enormous potential for a myriad of clinical uses. Electrochemical immunosensors provide a specific and simple analytical alternative as evidenced by their brief analysis times, inexpensive instrumentation, lower assay cost as well as good portability and amenability to miniaturization. The role nanomaterials play in biosensors, their ability to improve detection capabilities in low concentration analytes yielding clinically useful data and their impact on other biosensor performance properties will be discussed. Finally, the most common types of electroanalytical detection methods will be briefly touched upon.

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“…Results showed that the DLs for AFP, AFP-L3 and APT were 8.3, 4.7 and 6.0 pg mL −1 , respectively. Simultaneous detection of two cancer biomarkers, PSA and Interleukin-8 (IL-8) was also reported (Wan et al, 2011). A disposable SPCE array was used as the detection platform.…”

Section: +mentioning

confidence: 99%

Detection of Cancer Biomarkers With Nanotechnology

Zhang

et al. 2013

American Journal of Biochemistry and Biotechnology

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Early detection of cancer biomarkers with high precision is critically important for cancer therapy. A variety of sensors based on different nanostructured materials have attracted intensive research interest due to their potential for highly sensitive and selective detection of cancer biomarkers. This review covers the use of a variety of nanostructured materials, including carbon nanotubes, silicon nanowires, gold nanoparticles and quantum dots, in the fabrication of sensors. Emphases are placed on how the detection systems work and what detection limits can be achieved. Some assays described in this review outperform established methods for cancer biomarker detection. It is highly promising that these sensors would soon move into commercial-scale production and find routine use in hospitals.

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Carbon nanotube-based ultrasensitive multiplexing electrochemical immunosensor for cancer biomarkers

Cited by 152 publications

References 41 publications

A Biotin-streptavidin-enhanced Carbon Nanotube Amplification Strategy for an Ultrasensitive Immunodetection of Polybrominated Diphenyl Ethers

A Biotin-streptavidin-enhanced Carbon Nanotube Amplification Strategy for an Ultrasensitive Immunodetection of Polybrominated Diphenyl Ethers

Nanomaterial-Based Electrochemical Immunosensors for Clinically Significant Biomarkers

Detection of Cancer Biomarkers With Nanotechnology

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