Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering

Roshanbinfar, Kaveh; Mohammadi, Zahra; Mesgar, Abdorreza S.; Dehghan, Mohammad Mehdi; Oommen, Oommen P.; Hilborn, Jöns; Engel, Felix B.

doi:10.1039/c9bm00434c

Cited by 96 publications

(91 citation statements)

References 58 publications

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“…Yet, in this study, local compaction was associated with light arrhythmia. Further studies will show if this hampers the functionality and whether this can for example be compensated by the inclusion of electroconductive materials 10,55 .…”

Section: Discussionmentioning

confidence: 99%

Recombinant spider silk protein eADF4(C16)-RGD coatings are suitable for cardiac tissue engineering

Kramer

Aigner

Petzold

et al. 2020

Sci Rep

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Cardiac tissue engineering is a promising approach to treat cardiovascular diseases, which are a major socioeconomic burden worldwide. An optimal material for cardiac tissue engineering, allowing cardiomyocyte attachment and exhibiting proper immunocompatibility, biocompatibility and mechanical characteristics, has not yet emerged. An additional challenge is to develop a fabrication method that enables the generation of proper hierarchical structures and constructs with a high density of cardiomyocytes for optimal contractility. Thus, there is a focus on identifying suitable materials for cardiac tissue engineering. Here, we investigated the interaction of neonatal rat heart cells with engineered spider silk protein (eADF4(C16)) tagged with the tripeptide arginyl-glycyl-aspartic acid cell adhesion motif RGD, which can be used as coating, but can also be 3D printed. Cardiomyocytes, fibroblasts, and endothelial cells attached well to eADF4(C16)-RGD coatings, which did not induce hypertrophy in cardiomyocytes, but allowed response to hypertrophic as well as proliferative stimuli. Furthermore, Kymograph and MUSCLEMOTION analyses showed proper cardiomyocyte beating characteristics on spider silk coatings, and cardiomyocytes formed compact cell aggregates, exhibiting markedly higher speed of contraction than cardiomyocyte mono-layers on fibronectin. The results suggest that eADF4(C16)-RGD is a promising material for cardiac tissue engineering.

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Section: Discussionmentioning

confidence: 99%

Recombinant spider silk protein eADF4(C16)-RGD coatings are suitable for cardiac tissue engineering

Kramer

Aigner

Petzold

et al. 2020

Sci Rep

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“…Characterizations —hiPSC‐Derived Cardiomyocytes : F1 hiPSCs (90% confluent) at passage number 24 were cultured and differentiated into cardiomyocytes utilizing CHIR‐99 021 (8 × 10 −6 m ) and IWR‐1‐endo (5 × 10 −6 m ) as previously described . After the end of the differentiation protocol (day 12), cells were cultured for an additional 2 weeks in RPMI‐1640 supplemented with B27 (1X), and the medium was changed every other day.…”

Section: Methodsmentioning

confidence: 99%

Nanofibrous Composite with Tailorable Electrical and Mechanical Properties for Cardiac Tissue Engineering

Roshanbinfar

Vogt

Ruther

et al. 2019

Adv Funct Materials

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Cardiac tissue engineering is a promising strategy to prevent functional deterioration or even to enhance cardiac function upon myocardial infarction. Here, electrospun fiber mats containing different combinations of electrically conductive polyaniline, collagen, and/or hyaluronic acid are assessed regarding material properties and compatibility with cardiomyocyte attachment and function. Microstructure analysis reveals that collagen fiber mats contain a wide range of fiber diameters after crosslinking (from ≈300 nm to ≈5 µm); all other fiber mats contain fibers in the range of ≈120 to ≈300 nm. Fiber mats exhibit comparable electrical conductivity to and greater mechanical properties than the native human myocardium, which is considered beneficial. Cell–matrix interaction analysis utilizing postnatal rat cardiomyocytes reveals that the fiber mats are non‐cytotoxic and permit cell attachment and contraction. Fiber mats containing collagen (9.89%), hyaluronic acid (1.1%), and polyaniline (PANi, 1.34%) exhibit the most favorable properties with longer contraction time, higher contractile amplitude, and lower beating rates. Improved contraction is accompanied by increased connexin 43 expression. Importantly, this fiber mat is a suitable material for human‐induced pluripotent stem cell–derived cardiomyocytes regarding cytotoxicity, cell attachment, and function. Collectively, these data demonstrate that fiber mats made of collagen, hyaluronic acid, and polyaniline are promising materials for cardiac tissue engineering.

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“…Embedding hiPSC-CMs with collagen gel into PDMS channel to form a wire-like structure and exposing the tissues to electrical stimuli generate functionally more matured cardiac tissue. To further augment electrical conduction, electrically conductive silicon nanowires or carbon nanotubes were incorporated into hiPSC-CMs spheroids to form an electrically conductive environment (Tan et al, 2015;Roshanbinfar et al, 2019) that is later enhanced by the addition of an exogenous electrical stimulation (Richards et al, 2016). The regimens of electrical stimulation were explored by several groups to achieve T-tubule formation and positive force-frequency relationships (Hirt et al, 2014;Godier-Furnémont et al, 2015).…”

Section: D Cultures With Biophysical Stimulimentioning

confidence: 99%

A Brief Review of Current Maturation Methods for Human Induced Pluripotent Stem Cells-Derived Cardiomyocytes

Ahmed¹,

Anzai²,

Chanthra³

et al. 2020

Front. Cell Dev. Biol.

170

154

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Cardiovascular diseases are the leading cause of death worldwide. Therefore, the discovery of induced pluripotent stem cells (iPSCs) and the subsequent generation of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) was a pivotal point in regenerative medicine and cardiovascular research. They constituted an appealing tool for replacing dead and dysfunctional cardiac tissue, screening cardiac drugs and toxins, and studying inherited cardiac diseases. The problem is that these cells remain largely immature, and in order to utilize them, they must reach a functional degree of maturity. To attempt to mimic in vivo environment, various methods including prolonging culture time, co-culture and modulations of chemical, electrical, mechanical culture conditions have been tried. In addition to that, changing the topology of the culture made huge progress with the introduction of the 3D culture that closely resembles the in vivo cardiac topology and overcomes many of the limitations of the conventionally used 2D models. Nonetheless, 3D culture alone is not enough, and using a combination of these methods is being explored. In this review, we summarize the main differences between immature, fetal-like hiPSC-CMs and adult cardiomyocytes, then glance at the current approaches used to promote hiPSC-CMs maturation. In the second part, we focus on the evolving 3D culture model-it's structure, the effect on hiPSC-CMs maturation, incorporation with different maturation methods, limitations and future prospects.

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Carbon nanotube doped pericardial matrix derived electroconductive biohybrid hydrogel for cardiac tissue engineering

Abstract: Biohybrid hydrogels consisting of solubilized nanostructured pericardial matrix and electroconductive positively charged hydrazide-conjugated carbon nanotubes provide a promising material for stem cell-based cardiac tissue engineering.

Cited by 96 publications

References 58 publications

Recombinant spider silk protein eADF4(C16)-RGD coatings are suitable for cardiac tissue engineering

Recombinant spider silk protein eADF4(C16)-RGD coatings are suitable for cardiac tissue engineering

Nanofibrous Composite with Tailorable Electrical and Mechanical Properties for Cardiac Tissue Engineering

A Brief Review of Current Maturation Methods for Human Induced Pluripotent Stem Cells-Derived Cardiomyocytes

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