Carbon nanotube‐enhanced separation of DNA fragments by a portable capillary electrophoresis system with contactless conductivity detection

Xu, Yan; Li, Sam Fong Yau

doi:10.1002/elps.200600270

Cited by 66 publications

(52 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Initially, potential gradient detection was employed for the analysis of DNA fragments [35] and the water-soluble antibiotic neomycin [36]. The instrument was then equipped with a C 4 D for the detection of DNA fragments [37] and samples containing organic acids and chlorinated herbicides [21].…”

Section: Introductionmentioning

confidence: 99%

Identification of inorganic ions in post‐blast explosive residues using portable CE instrumentation and capacitively coupled contactless conductivity detection

et al. 2008

View full text Add to dashboard Cite

Novel CE methods have been developed on portable instrumentation adapted to accommodate a capacitively coupled contactless conductivity detector for the separation and sensitive detection of inorganic anions and cations in post-blast explosive residues from homemade inorganic explosive devices. The methods presented combine sensitivity and speed of analysis for the wide range of inorganic ions used in this study. Separate methods were employed for the separation of anions and cations. The anion separation method utilised a low conductivity 70 mM Tris/70 mM CHES aqueous electrolyte (pH 8.6) with a 90 cm capillary coated with hexadimethrine bromide to reverse the EOF. Fifteen anions could be baseline separated in 7 min with detection limits in the range 27-240 mg/L. A selection of ten anions deemed most important in this application could be separated in 45 s on a shorter capillary (30.6 cm) using the same electrolyte. The cation separation method was performed on a 73 cm length of fused-silica capillary using an electrolyte system composed of 10 mM histidine and 50 mM acetic acid, at pH 4.2. The addition of the complexants, 1 mM hydroxyisobutyric acid and 0.7 mM 18-crown-6 ether, enhanced selectivity and allowed the separation of eleven inorganic cations in under 7 min with detection limits in the range 31-240 mg/L. The developed methods were successfully field tested on post-blast residues obtained from the controlled detonation of homemade explosive devices. Results were verified using ion chromatographic analyses of the same samples.

show abstract

Section: Introductionmentioning

confidence: 99%

Identification of inorganic ions in post‐blast explosive residues using portable CE instrumentation and capacitively coupled contactless conductivity detection

et al. 2008

View full text Add to dashboard Cite

show abstract

“…3). The system is enclosed in a compact attaché case and connected to a laptop [56][57][58][59][60][61][62][63]. Li et al presented the first version in 2001 for environmental applications [63].…”

Section: Current Commercial Portable Instrumentsmentioning

confidence: 99%

“…A second version of the system extended the detection options and the PGD provided [58,61,63] with the possibility of connection to other different types of detector {e.g., spectrophotometric UV-Vis [57,60], contactless conductivity [56,59], or a custom-built photometric detector using light emitting diodes (LEDs) [62]} . Even though the option of the several external detectors brought versatility in detection modes, a significant increase in weight to 10 kg was reported.…”

Section: Current Commercial Portable Instrumentsmentioning

confidence: 99%

Portable capillary-based (non-chip) capillary electrophoresis

Ryvolová

Preisler

Brabazon

et al. 2010

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

Miniaturized, portable instrumentation has been gaining popularity in all areas of analytical chemistry. Capillary electrophoresis (CE), due to its main strengths of high separation efficiency, relatively short analysis time and low consumption of chemicals, is a particularly suitable technique for use in portable analytical instrumentation. In line with the general trend in miniaturization in chemistry utilizing microfluidic chips, the main thrust of portable CE (P-CE) systems development is towards chip-based miniaturized CE. Despite this, capillary-based (non-chip) P-CE systems have certain unmatched advantages, especially in the relative simplicity of the regular cylindrical geometry of the CE capillary, maximal volume-to-surface ratio, no need to design and to fabricate a chip, the low costs of capillary compared to chip, and better performance with some detection techniques. This review presents an overview of the state of the art of P-CE and literature relevant to future developments. We pay particular attention to the development and the potential of miniaturization of functional parts for P-CE. These include components related to sample introduction, separation and detection, which are the key elements in P-CE design. The future of P-CE may be in relatively simple, rugged designs (e.g., using a short piece of capillary fixed to a chip-sized platform on which injection and detection parts can be mounted). Electrochemical detection is well suited for miniaturization, so is probably the most suitable detection technique for P-CE, but optical detection is gaining interest, especially due to miniaturized light sources (e.g., light-emitting diodes).

show abstract

“…It usually takes a lot of time and effort, however, to test and develop a new polymer matrix [23]. Recently, addition of certain additives [22][23][24][25][26][27][28][29][30][31][32][33] into low-viscous polymer solutions has proved to be a very efficient and simple method to overcome the difficulty of filling capillaries and improve dsDNA separation performance, as described previously [34]. These examples indicate that the additives that promote chemical or physical cross-linking are required for good resolutions [32].…”

Section: Introductionmentioning

confidence: 97%

Effects of novel quasi‐interpenetrating network/gold nanoparticles composite matrices on DNA sequencing performances by CE

et al. 2007

View full text Add to dashboard Cite

Gold nanoparticles (GNPs) with particle sizes of about 20, 40, and 60 nm were prepared and added into a quasi-interpenetrating network (quasi-IPN) composed of linear polyacrylamide (LPA) with different viscosity-average molecular masses of 1.5, 3.3, and 6.5 MDa and poly-N,N-dimethylacrylamide (PDMA) to form polymer/metal composite matrices, respectively. These novel matrices could improve ssDNA sequencing performances due to interactions between GNPs and polymer chains and the formation of physical cross-linking points as demonstrated by intrinsic viscosities and glass transition temperatures. The effects of the parameters in relation to quasi-IPN/GNPs matrices, such as GNP contents, GNP particle sizes, LPA molecular masses, and solution concentrations, on ssDNA sequencing performances were studied. In the presence of GNPs, the separation had the advantages of high resolution, speediness, excellent reproducibility, long shelf life and easy automation. Therefore, less viscous matrix solutions (with moderate size GNPs) due to lower solution concentration and lower-molecular-mass LPA could be used to replace more viscous solutions (without GNPs) due to higher solution concentration or higher-molecular-mass LPA to separate DNA, while the sieving performances were approximate even higher, which helped to achieve full automation especial for capillary array electrophoresis (CAE) and microchip electrophoresis (MCE).

show abstract

Carbon nanotube‐enhanced separation of DNA fragments by a portable capillary electrophoresis system with contactless conductivity detection

Cited by 66 publications

References 14 publications

Identification of inorganic ions in post‐blast explosive residues using portable CE instrumentation and capacitively coupled contactless conductivity detection

Identification of inorganic ions in post‐blast explosive residues using portable CE instrumentation and capacitively coupled contactless conductivity detection

Portable capillary-based (non-chip) capillary electrophoresis

Effects of novel quasi‐interpenetrating network/gold nanoparticles composite matrices on DNA sequencing performances by CE

Contact Info

Product

Resources

About