Carbon Nanotube-Mediated Wireless Cell Permeabilization: Drug and Gene Uptake

Raffa, Vittoria; Gherardini, Lisa; Vittorio, Orazio; Bardi, Giuseppe; Ziaei, A.; Pizzorusso, Tommaso; Riggio, Cristina; Nitodas, Stephanos F.; Karachalios, Theodoros; Al‐Jamal, Khuloud T.; Kostarelos, Kostas; Costa, Mario; Cuschieri, Alfred

doi:10.2217/nnm.11.62

Cited by 32 publications

(12 citation statements)

References 90 publications

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“…47 A recent report suggested that exposure to electromagnetic waves promotes CNT entry not only into the cytoplasm of cells, but also into the nucleus. 50 Our present findings suggest a similarity between the modes of action of humic macromolecules with analogous biomacromolecules/proteins. The macromolecule acts as shuttle-like agent to enhance the permeability of [CNT/ organic] into the internal cellular compartments.…”

Section: A Physicochemical Mechanismsupporting

confidence: 63%

Induction of micronuclei by multi-walled carbon nanotubes interacting with humic acids in cultured human lymphocytes

Vidali

Bletsa

Kouloumpis

et al. 2016

Environ. Sci.: Nano

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Mixtures of multi-walled carbon nanotubes (MWCNTs) with natural humic acids (Leonardite humic acid, LHA) or humic acid-like polycondensates (HALP) were evaluated, for the first time, about their potential genotoxic and cytotoxic effects in cultured human lymphocytes. The genotoxic evaluation of the tested materials, either separately or in combination, for the detection of micronuclei (MN) in the cytoplasm of interphase cells, was performed using the cytokinesis block micronucleus (CBMN) assay. A comparative analysis of the genotoxicity and cytotoxicity reveals that in the tested concentrations, the [MWCNTs + LHA] mixture is more genotoxic and slightly more cytotoxic than the [MWCNTs + HALP] mixture. MN induction observed in human lymphocytes demonstrates that humic substances enhance the genotoxic effects of MWCNTs. In addition, the present data highlight aso far unforeseenpotential genotoxic effect as the result of both clastogenic and aneugenic actions of the particular mixtures on human lymphocytes.Environ. Sci.: Nano This journal is A genotoxicity mechanism of multi-walled carbon nanotubes (MWCNTs) interacting with humic acids (HAs) is revealed. The interfacial properties of MWCNT-HA formations were characterized with ATR-FTIR, Atomic Force Microscopy and Dynamic Light Scattering. Using natural and synthetic/metal-free humic polymers, the mechanisms of colloidal dispersion are correlated with the revealed genotoxicity. Moreover, the geno-vs. cytotoxicity phenomena are quantitatively distinguished. These phenomena originate from the action of humic macromolecules as "chaperones" that shuttle MWCNTs into the cell compartments.

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Section: A Physicochemical Mechanismsupporting

confidence: 63%

Induction of micronuclei by multi-walled carbon nanotubes interacting with humic acids in cultured human lymphocytes

Vidali

Bletsa

Kouloumpis

et al. 2016

Environ. Sci.: Nano

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“…Excitation of CNTs by exposure to an appropriately tuned radio frequency field has been shown to permeabilize cell membranes to allow cell uptake of normally impermeable drugs or gene expression vectors both in in vitro and in vivo in brain tissue [101]. Similarly, treatment of cancer cells or tumor bearing mice using SWNTs chemically conjugated with platinum-based chemotherapeutics [102] or loaded with doxorubicin by hydrophobic interactions [103] and combined with NIR mediated photothermal heating were significantly more effective than either therapy alone.…”

Section: Cnt-mediated Thermal Therapy: Rationale and Current Statusmentioning

confidence: 99%

Carbon nanotubes in hyperthermia therapy

Singh

Torti

2013

Advanced Drug Delivery Reviews

203

156

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Thermal tumor ablation therapies are being developed with a variety of nanomaterials, including single-and multiwalled carbon nanotubes. Carbon nanotubes (CNTs) have attracted interest due to their potential for simultaneous imaging and therapy. In this review, we highlight in vivo applications of carbon nanotube-mediated thermal therapy (CNMTT) and examine the rationale for use of this treatment in recurrent tumors or those resistant to conventional cancer therapies. Additionally, we discuss strategies to localize and enhance the cancer selectivity of this treatment and briefly examine issues relating the toxicity and long term fate of CNTs.

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“…Similarly, oxidative stress-induced DNA damage was also observed after an intraperitoneal administration of IONPs in mice 18,40 . However, few studies also reported no genotoxicity induced by IONPs 41,42 . In our study, we found that IONPs exposure to Wistar rats increases the frequency of both chromosomal aberration and micronucleus induction along with increased DNA damage in a dose-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of DNA interaction, genotoxicity and oxidative stress induced by iron oxide nanoparticles both in vitro and in vivo: attenuation by thymoquinone

Ansari

Parveen

Ahmad

et al. 2019

Sci Rep

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Iron oxide nanoparticles (IONPs) are known to induce cytotoxicity in various cancer cell lines through the generation of reactive oxygen species (ROS). However, the studies on its potential to induce toxicity in normal cell lines and in vivo system are limited and ambiguity still exists. Additionally, small molecules are known to interact with the DNA and cause damage to the DNA. The present study is designed to evaluate the potential interaction of IONPs with DNA along with their other toxicological effects and subsequent attenuation by thymoquinone both in vitro (primary lymphocytes) and in vivo (Wistar rats). IONPs were characterized by TEM, SEM-EDS, and XRD. The results from DNA interaction studies showed that IONPs formed a complex with DNA and also got intercalated between the base pairs of the DNA. The decrease in percent cell viability of rat’s lymphocytes was observed along with an increase in ROS generation in a dose-dependent manner (50, 100, 200, 400 and 800 μg/ml of IONPs). The genetic damage in in vivo might be due to the generation of ROS as depletion in anti-enzymatic activity was observed along with an increase in lipid peroxidation in a dose–dependent manner (25, 50, 100 mg/kg of IONPs). Interestingly, supplementation of thymoquinone in combination with IONPs has significantly ( P < 0.05) attenuated the genetic and oxidative damage in a dose-dependent manner both in vitro and in vivo . It can be concluded that thymoquinone has the potential to attenuate the oxidative stress and genetic toxicity in vitro and in vivo .

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Carbon Nanotube-Mediated Wireless Cell Permeabilization: Drug and Gene Uptake

Abstract: The results showed that MAN has desirable in vivo biocompatibility, presenting potential for use as a MDDS, especially in CNS disease therapy.

Cited by 32 publications

References 90 publications

Induction of micronuclei by multi-walled carbon nanotubes interacting with humic acids in cultured human lymphocytes

Induction of micronuclei by multi-walled carbon nanotubes interacting with humic acids in cultured human lymphocytes

Carbon nanotubes in hyperthermia therapy

Evaluation of DNA interaction, genotoxicity and oxidative stress induced by iron oxide nanoparticles both in vitro and in vivo: attenuation by thymoquinone

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