Carbon nanotube poroshell silica as a novel stationary phase for fast HPLC analysis of monoclonal antibodies

Abstract

A new carbon nanotube porous silica poroshell stationary phase was developed. The chromatographic support was coated with ultrashort single-wall carbon nanotubes (SWCNTs) in a noncovalent way. It was demonstrated that the porous amino silica surface of the 300 NH2 poroshell column stabilized with 1-methyl-2-pyrrolidinone efficiently and stably adsorbed SWCNTs onto the chromatographic support. It was shown that this novel poroshell carbon nanotube (CNT) stationary phase was very useful for the HPLC separation of a series of monoclonal antibodies (mAbs) in a short analysis time (<3 min). The high-performance liquid chromatography (HPLC) method was validated and was successfully tested for the fast quantitative and qualitative control of chemotherapeutic bags fabricated in a hospital pharmacy.

Nanomaterials as stationary phases and supports in liquid chromatography

“…CNTs have been of interest as stationary phases because of their good stability and their ability to be used as either individual nanoparticles or as aggregates that can undergo various interactions with solutes, including dipole‐dipole interactions, hydrogen bonding, π‐π stacking, and nonpolar interactions, among others . These properties have been found in a number of studies to provide stationary phases based on CNTs with excellent long‐term stabilities and the ability to provide improved retention, resolution, and analysis times for a number of compound classes when compared to traditional LC columns , such as those containing C 18 ‐ or aminopropyl‐silica (e.g., see Sections –) .…”

“…Another advantage of using adsorption is it can allow multiple layers of nanoparticles to be placed onto a support , which can lead to improved retention and resolution . Adsorption through electrostatic interactions has been used for immobilizing materials such as gold nanoparticles , nanomaterials based on zirconia and titanium oxide , and latex nanoparticles onto silica or organic monoliths . A few specific examples are the adsorption of gold nanoparticles onto silica that has been stabilized with L‐cysteine or L‐cysteine methyl ester and the adsorption of zirconia nanoparticles directly, or through alternating layers of these nanoparticles and sodium dodecyl sulfate, on the surface of silica particles .…”

“…Other non‐covalent interactions (e.g., dipole‐dipole interactions and dispersion interactions) have also been utilized to adsorb nanoparticles onto or within supports such as silica and organic monoliths . Examples for carbon‐based nanomaterials include the adsorption of SWCNTs to aminopropyl silica or related materials , the layering of hydroxyl‐MWCNTs on silica , and the adsorption of oxidized and carboxylated MWCNTs on an amine‐activated GMA/EDMA co‐polymer .…”

“…Advantages of reversed‐phase LC include its ability to work directly with aqueous samples and its ability to retain a wide range of compounds based on the general property of their polarity. Both carbon‐based nanomaterials and inorganic nanomaterials have been examined as tools to alter the retention or selectivity of reversed‐phase separations and the physical or chemical properties of the supports that can be used in these separations .…”

A Novel Fluorinated Boron Nitride Nanotube Organic Monolithic Column for Capillary Liquid Chromatography

“…The specific properties of nanoparticles such as carbon (or boron nitride) nanotubes are of particular interest in separation science [1][2][3][4][5]. They could improve the analytical performances of the chromatographic methods such as higher resolution and chromatographic retention or better repeatability and stability due to the specific 1 3…”