Carbon nanotubes non-covalently functionalized with cytochrome c: A new bioanalytical platform for building bienzymatic biosensors

Eguílaz, Marcos; Venegas, Constanza J.; Gutiérrez, Alejandro García; Rivas, Gustavo A.; Bollo, Soledad

doi:10.1016/j.microc.2016.04.018

Cited by 24 publications

(8 citation statements)

References 18 publications

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“…CNTs were also physically functionalized with cytochrome to construct a new bioanalytical platform for building biosensors. The sensitivity of the resulting biosensor toward glucose has showed a high reproducibility and a successful determination of glucose in commercial beverages [21].…”

Section: Small Molecule Anchoring Of Cntsmentioning

confidence: 99%

Surface Functionalization of Carbon Nanotubes for Energy Applications

Berber¹,

Hafez²,

Mustafa³

2019

Perspective of Carbon Nanotubes

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Carbon nanotubes (CNTs) are receiving a great deal of attention as a catalyst support for different energy applications, due to their high surface area and high conductivity. Recent literature studies have shown that the application of CNTs mainly depends on their surface functionalization process. Typically, pristine CNTs (as produced) have no functional groups, which is usually considered as an obstacle to their widespread application. In this chapter, we highlight the different techniques used to functionalize the surface of CNTs, including physical and chemical functionalization processes. We show the advantages and the drawbacks of the different functionalization processes. Additionally, we explain in detail the different techniques used to characterize the CNTs before and after functionalization processes. Furthermore, we focus on polymer wrapping techniques of CNTs to create active nanocomposite materials for energy applications, in particular the applications in the agriculture field to fight pollution and make farming activity easier and more efficient.

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Section: Small Molecule Anchoring Of Cntsmentioning

confidence: 99%

Surface Functionalization of Carbon Nanotubes for Energy Applications

Berber¹,

Hafez²,

Mustafa³

2019

Perspective of Carbon Nanotubes

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“…Eguílaz et al. described a glucose biosensor prepared by modification of GCE with MWCNTs dispersed in cyt c and casted with GOx using hydroquinone as redox mediator. Caro‐Jara et al.…”

Section: Introductionmentioning

confidence: 99%

“…Xiang et al [24] proposed a micromolar bienzymatic detection of glucose through the direct electron transfer of cytochrome c (cyt c) on gold nanoparticles/poly(aniline) nanospheres composite. Eguílaz et al [25] described a glucose biosensor prepared by modification of GCE with MWCNTs dispersed in cyt c and casted with GOx using hydroquinone as redox mediator. Caro-Jara et al [26] reported an amperometric bienzymatic biosensor based on GOx and HRP immobilized on mesoporous silica with hexagonal symmetry (MCM-41) using Nafion as co-immobilizing reagent and catechol as redox mediator.…”

Section: Introductionmentioning

confidence: 99%

Avidin and Glucose Oxidase‐non‐covalently Functionalized Multi‐walled Carbon Nanotubes: A New Analytical Tool for Building a Bienzymatic Glucose Biosensor

et al. 2019

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We report an innovative supramolecular architecture for bienzymatic glucose biosensing based on the non‐covalently functionalization of multi‐walled carbon nanotubes (MWCNTs) with two proteins, glucose oxidase (GOx) (to recognize glucose) and avidin (to allow the specific anchoring of biotinylated horseradish peroxidase (b‐HRP)). The optimum functionalization was obtained by sonicating for 10 min 0.50 mg mL−1 MWCNTs in a solution of 2.00 mg mL−1 GOx+1.00 mg mL−1avidin prepared in 50 : 50 v/v ethanol/water. The sensitivity to glucose for glassy carbon electrodes (GCE) modified with MWCNTs‐GOx‐avidin dispersion and b‐HRP (GCE/MWCNTs‐GOx‐avidin/b‐HRP), obtained from amperometric experiments performed at −0.100 V in the presence of 5.0×10−4 M hydroquinone, was (4.8±0.3) μA mM−1 (r2=0.9986) and the detection limit was 1.2 μM. The reproducibility for 5 electrodes using the same MWCNTs/GOx‐avidin dispersion was 4.0 %, while the reproducibility for 3 different dispersions and 9 electrodes was 6.0 %. The GCE/MWCNT‐GOx‐avidin/b‐HRP was successfully used for the quantification of glucose in a pharmaceutical product and milk.

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“…In this regard, the use of (bio)polymers as CNTs‐functionalization agents have demonstrated to be extremely useful . We have reported the use of polyethylenimine , polylysine , polyhistidine , glucose oxidase , calf‐thymus double stranded DNA and cytochrome c for the highly successful non‐covalent functionalization of multi‐wall carbon nanotubes (MWCNTs). Polytyrosine covalently attached to single‐wall carbon nanotubes (SWCNTs) have also demonstrated to be highly effective for the preparation of electrochemical sensors .…”

Section: Introductionmentioning

confidence: 99%

Non‐covalent Functionalization of Multi‐wall Carbon Nanotubes with Polyarginine: Characterization and Analytical Applications for Uric Acid Quantification

Gutiérrez

Eguílaz

et al. 2018

Electroanalysis

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We report for the first time the non‐covalent functionalization of multiwall carbon nanotubes (MWCNTs) with polyarginine (Polyarg), the modification of glassy carbon electrodes (GCE) with the resulting Polyarg‐MWCNTs dispersion and the analytical application of Polyarg‐MWCNTs‐modified GCE for the quantification of uric acid. The optimum MWCNT‐Polyarg dispersion was obtained by sonicating for 5.0 min the mixture of 0.75 mg mL−1 MWCNTs and 0.50 mg mL−1 Polyarg. The dispersion was characterized by scanning electron microscopy, electrophoretic mobility, electrochemical impedance spectroscopy, cyclic voltammetry and amperometry. The presence of MWCNT‐Polyarg at GCE surface produced a drastic decrease in the overvoltages for the oxidation of hydrogen peroxide (300 mV) ascorbic acid (281 mV) and uric acid (70 mV) and for the reduction of hydrogen peroxide (200 mV), as well as an important decrease in the charge transfer resistances for hydrogen peroxide, hydroquinone/quinone and ferricyanide/ferrocyanide markers. The strong adsorption of uric acid at GCE/MWCNT‐Polyarg made possible the highly sensitive detection of this biomarker at nanomolar levels even in the presence of 1.0×10−4 M ascorbic acid by Adsorptive Stripping with medium exchange and linear scan voltammetry transduction. The quantification of uric acid in untreated human urine was very successful, demonstrating an excellent correlation (98 %) with the reference method used in clinical laboratories (Uricostat, Wiener Lab).

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Carbon nanotubes non-covalently functionalized with cytochrome c: A new bioanalytical platform for building bienzymatic biosensors

Cited by 24 publications

References 18 publications

Surface Functionalization of Carbon Nanotubes for Energy Applications

Surface Functionalization of Carbon Nanotubes for Energy Applications

Avidin and Glucose Oxidase‐non‐covalently Functionalized Multi‐walled Carbon Nanotubes: A New Analytical Tool for Building a Bienzymatic Glucose Biosensor

Non‐covalent Functionalization of Multi‐wall Carbon Nanotubes with Polyarginine: Characterization and Analytical Applications for Uric Acid Quantification

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