Carbon nanotubes: Plant gene delivery and genome editing

Chandrasekaran, Rajkuberan; Periakaruppan, Rajiv; Abd‐Elsalam, Kamel A.

doi:10.1016/b978-0-12-819786-8.00014-1

Cited by 23 publications

(17 citation statements)

References 75 publications

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“…In general, this method is preferred for rapid assays using transient expressions, such as protein localization, the functional analysis of promoters, and transcription factor characterization (Lenka et al, 2015(Lenka et al, , 2018Wang et al, 2018). The particle bombardment method enables the delivery of DNA sequences > 150 kb, albeit with the possible compromise in DNA integrity (Chandrasekaran et al, 2020). Moreover, gene gun-mediated plant transformation can result in scrambled and multiple integrations (Altpeter et al, 2005;.…”

Section: Conventional Plant Biomolecule Delivery Approaches and Their Limitationsmentioning

confidence: 99%

“…Nanotechnology-based methods have been proposed as inexpensive, easy, and robust techniques to transfer genes or other molecules into plants with high efficiency and low toxicity (Chandrasekaran et al, 2020). Nanotechnology has significantly impacted various research fields, including medicine, energy, and manufacturing.…”

Section: Advanced Plant Biomolecule Delivery Approaches Via the Application Of Nanobiotechnologymentioning

confidence: 99%

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Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

et al. 2021

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Agriculture is an important source of human food. However, current agricultural practices need modernizing and strengthening to fulfill the increasing food requirements of the growing worldwide population. Genome editing (GE) technology has been used to produce plants with improved yields and nutritional value as well as with higher resilience to herbicides, insects, and diseases. Several GE tools have been developed recently, including clustered regularly interspaced short palindromic repeats (CRISPR) with nucleases, a customizable and successful method. The main steps of the GE process involve introducing transgenes or CRISPR into plants via specific gene delivery systems. However, GE tools have certain limitations, including time-consuming and complicated protocols, potential tissue damage, DNA incorporation in the host genome, and low transformation efficiency. To overcome these issues, nanotechnology has emerged as a groundbreaking and modern technique. Nanoparticle-mediated gene delivery is superior to conventional biomolecular approaches because it enhances the transformation efficiency for both temporal (transient) and permanent (stable) genetic modifications in various plant species. However, with the discoveries of various advanced technologies, certain challenges in developing a short-term breeding strategy in plants remain. Thus, in this review, nanobased delivery systems and plant genetic engineering challenges are discussed in detail. Moreover, we have suggested an effective method to hasten crop improvement programs by combining current technologies, such as speed breeding and CRISPR/Cas, with nanotechnology. The overall aim of this review is to provide a detailed overview of nanotechnology-based CRISPR techniques for plant transformation and suggest applications for possible crop enhancement.

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Section: Conventional Plant Biomolecule Delivery Approaches and Their Limitationsmentioning

confidence: 99%

Section: Advanced Plant Biomolecule Delivery Approaches Via the Application Of Nanobiotechnologymentioning

confidence: 99%

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

et al. 2021

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“…However, this can be rectified through functionalization of the nano particle which is known as modification of surface of nanoparticles that improves their properties and enables them to play a major role (Riley and Vermerris, 2017). Functionalized CNTs are less toxic and show higher gene expression (Mohajeri et al, 2019). Carbon nanotubes functionalized with polyethyleneimine (PEI) had potential to traverse cell and transfer the ssDNA-FITC into German chamomile (L Chamomilla M), hence, CNTs along with ultrasound showed improved gene transfer as they protect DNA from destructive enzymes and ultra sound waves (Ghaghelestany et al, 2020…”

Section: Carbon Based Nanoparticlesmentioning

confidence: 99%

“…Hence, Torney et al (2007) reported successful transformation by MSNP functionalized either with TEG (Triethylene glycol) or gold in mesophyll protoplast of tobacco (Nicotiana tabacum) and intact plant tissues such as tobacco cotyledon and immature maize embryos (Zea mays). Martin-Ortigosa et al (2014) reported that delivery of Cre recombinase protein by means of gold plated MSNP resulted excision of loxP flank genes from maize genome. These findings imply that MSNPs being a carrier material in transformation process, have developed potential to be used in genetically modified crops (Rastogi et al, 2019).…”

Section: Silica Based Nanoparticlementioning

confidence: 99%

A Review of Nanotechnology as a Novel Method of Gene Transfer in Plants

Begum

Jayawardana

2021

J Agric Sciences

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Purpose: Nanotechnology has evolved as an effective tool in numerous fields including agriculture, medicine and engineering. Recently it's potential as an alternative genetic transformation method has been identified. However, a comprehensive understanding over nanoparticles and their behavior in living cells is important to realize the full potential of this technology in biotechnological applications. Therefore, we review the application potential of widely employed nanoparticles in plant transformation here. Literature/Background: Development of new crop varieties with desirable traits via biotechnological applications is a solution for challenges associated with climate change and higher population growth.In such aspects, transformation of plant cells which is known as the process of changing one's genome by integration exogenous DNA, is an absolute necessity and results far better and improved stable characteristics in original. Rigid and multi layered cell wall impedes penetration of exterior biomolecules and hence causes the transformation process complicated. Even though, numerous conventional methods have been established for plant transformation, lower transformation efficiency, tissue damage and random integration of transgenes warrants the need for novel approaches. In this context, novel techniques have been explored and as a result nanoparticles have been found effective in transformation of protoplasts as well as intact plant cells. Nanoparticles internalized either via endocytosis or direct penetration release transgenes from nanoparticle-DNA complexes and result in transient or stable expression. Nanoparticles ensure higher transformation efficiency, no transgenic silencing and protection of biogenic molecules from degradation by intracellular nucleases.

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“…RNA or DNA viruses have also been used to introduce the gRNA into the plant system [ 161 , 162 ]. Similarly, carbon nanotube nanoparticles have also been used as an efficient plasmid DNA delivery mechanism [ 163 , 164 ]. Researchers have also used protoplasts to first test the cleaving ability of sgRNAs in vivo, thereby determining their efficiency prior to entering into full-scale transformation [ 165 , 166 ].…”

Section: Introductionmentioning

confidence: 99%

Improving Horticultural Crops via CRISPR/Cas9: Current Successes and Prospects

Bhatta

Malla²

2020

Plants

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Horticultural crops include a diverse array of crops comprising fruits, vegetables, nuts, flowers, aromatic and medicinal plants. They provide nutritional, medicinal, and aesthetic benefits to mankind. However, these crops undergo many biotic (e.g., diseases, pests) and abiotic stresses (e.g., drought, salinity). Conventional breeding strategies to improve traits in crops involve the use of a series of backcrossing and selection for introgression of a beneficial trait into elite germplasm, which is time and resource consuming. Recent new plant breeding tools such as clustered regularly interspaced short palindromic repeats (CRISPR) /CRISPR-associated protein-9 (Cas9) technique have the potential to be rapid, cost-effective, and precise tools for crop improvement. In this review article, we explore the CRISPR/Cas9 technology, its history, classification, general applications, specific uses in horticultural crops, challenges, existing resources, associated regulatory aspects, and the way forward.

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Carbon nanotubes: Plant gene delivery and genome editing

Cited by 23 publications

References 75 publications

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

Advantage of Nanotechnology-Based Genome Editing System and Its Application in Crop Improvement

A Review of Nanotechnology as a Novel Method of Gene Transfer in Plants

Improving Horticultural Crops via CRISPR/Cas9: Current Successes and Prospects

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