Carbon quantum dots—Annexin V probe: photoinduced electron transfer mechanism, phosphatidylserine detection, and apoptotic cell imaging

Mahani, Mohamad; Karimi-Mazidi, Parisa; Khakbaz, Faeze; Torkzadeh‐Mahani, Masoud

doi:10.1007/s00604-021-05147-8

Cited by 14 publications

(4 citation statements)

References 53 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Photo-induced electron transfer is an important mechanism for CDs in cell apoptosis imaging. Based on the strong interaction between Annexin V and phosphatidylserine, a non-toxic Annexin V-conjugated CD probe was created by Mahani et al [ 49 ]. The photoinduced electron transfer between the CDs and the tyrosine and tryptophan in the annexin structure caused the fluorescence of the CDs to be quenched in the absence of phosphatidylserine.…”

Section: Cds: Synthesis Properties and Mechanismmentioning

confidence: 99%

Carbon Dots: Opportunities and Challenges in Cancer Therapy

2023

View full text Add to dashboard Cite

Recently, carbon dots (CDs) have been actively studied and reported for their various properties. In particular, the specific characteristics of carbon dots have been considered as a possible technique for cancer diagnosis and therapy. This is also a cutting-edge technology that offers fresh ideas for treating various disorders. Though carbon dots are still in their infancy and have not yet shown their value to society, their discovery has already resulted in some noteworthy advancements. The application of CDs indicates conversion in natural imaging. Photography using CDs has demonstrated extraordinary appropriateness in bio-imaging, the discovery of novel drugs, the delivery of targeted genes, bio-sensing, photodynamic therapy, and diagnosis. This review seeks to provide a comprehensive understanding of CDs, including their benefits, characteristics, applications, and mode of action. In this overview, many CD design strategies will be highlighted. In addition, we will discuss numerous studies on cytotoxic testing to demonstrate the safety of CDs. The current study will address the production method, mechanism, ongoing research, and application of CDs in cancer diagnosis and therapy.

show abstract

Section: Cds: Synthesis Properties and Mechanismmentioning

confidence: 99%

Carbon Dots: Opportunities and Challenges in Cancer Therapy

2023

View full text Add to dashboard Cite

show abstract

“…To investigate the optical properties of NCD, fluorescence spectra and UV–Vis absorption spectra analyses were carried out. Figure 2 A indicates that NCD aqueous solution possesses optical absorption spectra in the UV–Vis region (black line), with a shoulder and a maximum peak at 235 and 330 nm, respectively, which can be related to the π - π * (C = C band) and n - π * (C = O band) transitions [ 42 – 44 ]. Furthermore, the fluorescence spectrum of NCD (blue line) exhibited an emission maximum at 445 nm ( λ ex = 350 nm), as shown in Fig.…”

Section: Resultsmentioning

confidence: 99%

Ultrasensitive FRET-based aptasensor for interleukin-6 as a biomarker for COVID-19 progression using nitrogen-doped carbon quantum dots and gold nanoparticles

et al. 2022

Self Cite

View full text Add to dashboard Cite

A label-free and specific FRET-based interleukin-6 (IL-6) aptasensor was developed using a DNA aptamer modified with nitrogen-doped carbon quantum dots (NCDs) and gold nanoparticles (AuNPs) as a donor-quencher pair. The assayed target was capable of disrupting the donor–acceptor assemblies yielding a concentration-related fluorescence recovery of NCDs ( λ em = 445 nm and λ ex = 350 nm). By designing two different probes, the interaction of DNA aptamers with IL-6 protein was studied using FRET efficiency. It appeared that the sensing probes showed slightly different sensing profiles. One of the aptasensors showed a linear response of 1.5–5.9 pg/mL for IL-6 with a coefficient of determination of R 2 ≥ 0.99 and the a detection limit of 0.82 pg/mL (at S/N = 3). The experimental results indicated that the biosensor can be applied to determine IL-6 in human serum (with recovery of 95.7–102.9%). Due to the high sensitivity, excellent selectivity, and simplicity of the procedure, this strategy represents a promising alternative for IL-6 sensing in clinical applications. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1007/s00604-022-05570-5.

show abstract

“…The detection of single apoptotic cells using luminol electrochemiluminescence has been reported. 134 Other studies use nanomaterials such as carbon quantum dots to design annexin V conjugated apoptosis detection probes, 21 or silver nanoparticles followed by SERS analysis to highlight externalized PS. 26 Radiolabelled annexin V was also used to detect apoptotic cells in vivo .…”

Section: Advanced Methods For the Detection Of Apoptosismentioning

confidence: 99%