Abstract. The eddy covariance technique, which is used in the determination of net ecosystem CO 2 exchange (NEE), is subject to significant errors when advection that carries CO 2 in the mean flow is ignored. We measured horizontal and vertical advective CO 2 fluxes at the Niwot Ridge AmeriFlux site (Colorado, USA) using a measurement approach consisting of multiple towers. We observed relatively high rates of both horizontal (F hadv ) and vertical (F vadv ) advective fluxes at low surface friction velocities (u * ) which were associated with downslope katabatic flows. We observed that F hadv was confined to a relatively thin layer (0-6 m thick) of subcanopy air that flowed beneath the eddy covariance sensors principally at night, carrying with it respired CO 2 from the soil and lower parts of the canopy. The observed F vadv came from above the canopy and was presumably due to the convergence of drainage flows at the tower site. The magnitudes of both F hadv and F vadv were similar, of opposite sign, and increased with decreasing u * , meaning that they most affected estimates of the total CO 2 flux on calm nights with low wind speeds. The mathematical sign, temporal variation and dependence on u * of both F hadv and F vadv were determined by the unique terrain of the Niwot Ridge site. Therefore, the patterns we observed may not be broadly applicable to other sites. We evaluated the influence of advection on the cumulative annual and monthly estimates of the total CO 2 flux (F c ), which is often used as an estimate of NEE, over six years using the dependence of F hadv and F vadv on u * . When the sum of F hadv and F vadv was used to correct monthly F c , we observed values that were different from the monthly F c calculated using the traditional u * -filter correction by À16 to 20 g CÁm À2 Ámo À1