Carbon source-dependent inhibition of xyl operon expression of the Pseudomonas putida TOL plasmid

Holtel, Andreas; Marqués, Silvia; Mohler, Isabel; Jakubzik, Ute; Timmis, Kenneth N.

doi:10.1128/jb.176.6.1773-1776.1994

Cited by 123 publications

(126 citation statements)

References 23 publications

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“…11 The inhibitory effect of carbon sources on biodegradation of styrene has been reported. 12,13 The effect of the presence of additional carbon sources observed in this study is consistent with the higher anaerobic degradation of LAS in the absence of additional sources of carbon that has been observed. 14 Similarly, sludge acclimatized to the degradation of phenol showed an initial preference for easily degradable co-substrates such as glucose with only a slow concomitant assimilation of phenol.…”

Section: Discussionsupporting

confidence: 78%

Effect of additional carbon source on biodegradation of linear alkylbenzene sulfonate by las-utilizing bacteria

Eniola¹

2011

J Xenobiotics

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Aerobic biodegradation of linear alkylbenzene sulfonate (LAS) by LAS-utilizing bacteria (LUB) in the presence of other sources of carbon (glucose and soluble starch) was examined. Biodegradation of LAS was monitored as primary degradation in terms of half-life (t½) of the surfactant. Biodegradation of LAS by the individual LUB was slower in the presence of Glucose. Biodegradation of the surfactant by the various consortia of LUB was slower in the presence of the carbon sources: t½ increased to 3 days. The rates of biodegradation by the consortia can be ranked as: four-membered (t½=9 days) > three-membered (t½=9 to 13 days) > two-membered consortia (t½=10 to 15 days). Generally, degradation in the presence of the carbon sources was faster with the consortia than the individual species. Degradation of the surfactant by the LUB was generally fastest in the absence of additional carbon sources. The possible role of additional carbon sources in persistence of surfactant in water bodies and the application of the observation in management of LAS-containing-effluent is suggested.

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Section: Discussionsupporting

confidence: 78%

Effect of additional carbon source on biodegradation of linear alkylbenzene sulfonate by las-utilizing bacteria

Eniola¹

2011

J Xenobiotics

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“…The inverse linear relationship between growth rates and the intracellular concentration of (p)ppGpp provides a simple interpretation of the previous finding of inverse correlation between Po transcription levels and growth rates (Sze et al, 1996). A positive role for (p)ppGpp may also, at least in part, explain catabolite repression on aromatic degradation observed with the 54 -dependent TOL toluene/xylene pathway (Duetz et al, 1994;Holtel et al, 1994) and the 54 -dependent phenol catabolic pathway of P. putida H (Mü ller et al, 1996). Although the generality of involvement of this metabolic signal has to be verified, it is our expectation that other 54 promoters reported to control specialized catabolic functions of soil and water microorganisms will fall into the (p)ppGpp regulon, fulfilling the same function as glucose repression in enterics, namely causing silencing of energetically less favourable specialized catabolic functions until needed.…”

Section: Discussionmentioning

confidence: 76%

The alarmone (p)ppGpp mediates physiological‐responsive control at the σ⁵⁴‐dependent Po promoter

Sze

Shingler

1999

Molecular Microbiology

103

129

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SummaryTranscription from the Pseudomonas-derived 54 -dependent Po promoter of the dmp operon is mediated by the aromatic-responsive regulator DmpR. However, physiological control is superimposed on this regulatory system causing silencing of the DmpR-mediated transcriptional response in rich media until the transition between exponential and stationary phase is reached. Here, the positive role of the nutritional alarmone (p)ppGpp in DmpR regulation of the Po promoter has been identified and investigated in vivo. Overproduction of (p)ppGpp in a Pseudomonas reporter system was found to allow an immediate transcriptional response under normally non-permissive conditions. Conversely (p)ppGpp-deficient Escherichia coli strains were found to be severely defective in DmpR-mediated transcription, demonstrating the requirement for this metabolic signal. A subset of mutations in the ␤, ␤Ј and 70 subunits of RNA polymerase, which confer prototrophy on ppGpp 0 E. coli, was also found to restore specific DmpR-mediated transcription from Po, suggesting that the metabolic signal is mediated directly through the 54 -RNA polymerase. These data provide a direct mechanistic link between the physiological status of the cell and expression from 54 promoters.

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“…In pseudomonads, glucose utilization was found to be repressed by organic acids, known as reverse CCR (Collier et al, 1996). Although CCR is not well studied in pseudomonads, glucose and organic acids have been reported to repress the enzymes and transport system involved in the utilization of several aromatic compounds (Duetz et al, 1994;Holtel et al, 1994;McFall et al, 1997;Müller et al, 1996;Ng & Dawes, 1973;Schleissner et al, 1994;Tiwari & Campbell, 1969).…”

Section: Introductionmentioning

confidence: 99%

Repression of the glucose-inducible outer-membrane protein OprB during utilization of aromatic compounds and organic acids in Pseudomonas putida CSV86

et al. 2011

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Pseudomonas putida CSV86 shows preferential utilization of aromatic compounds over glucose. Protein analysis and [ 14 C]glucose-binding studies of the outer membrane fraction of cells grown on different carbon sources revealed a 40 kDa protein that was transcriptionally induced by glucose and repressed by aromatics and succinate. Based on 2D gel electrophoresis and liquid chromatography-tandem mass spectrometry analysis, the 40 kDa protein closely resembled the porin B of P. putida KT2440 and carbohydrate-selective porin OprB of various Pseudomonas strains. The purified native protein (i) was estimated to be a homotrimer of 125 kDa with a subunit molecular mass of 40 kDa, (ii) displayed heat modifiability of electrophoretic mobility, (iii) showed channel conductance of 166 pS in 1 M KCl, (iv) permeated various sugars (mono-, di-and trisaccharides), organic acids, amino acids and aromatic compounds, and (v) harboured a glucosespecific and saturable binding site with a dissociation constant of 1.3 mM. These results identify the glucose-inducible outer-membrane protein of P. putida CSV86 as a carbohydrate-selective protein OprB. Besides modulation of intracellular glucose-metabolizing enzymes and specific glucose-binding periplasmic space protein, the repression of OprB by aromatics and organic acids, even in the presence of glucose, also contributes significantly to the strain's ability to utilize aromatics and organic acids over glucose.

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Carbon source-dependent inhibition of xyl operon expression of the Pseudomonas putida TOL plasmid

Cited by 123 publications

References 23 publications

Effect of additional carbon source on biodegradation of linear alkylbenzene sulfonate by las-utilizing bacteria

Effect of additional carbon source on biodegradation of linear alkylbenzene sulfonate by las-utilizing bacteria

The alarmone (p)ppGpp mediates physiological‐responsive control at the σ⁵⁴‐dependent Po promoter

Repression of the glucose-inducible outer-membrane protein OprB during utilization of aromatic compounds and organic acids in Pseudomonas putida CSV86

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Carbon source-dependent inhibition of xyl operon expression of the Pseudomonas putida TOL plasmid

Cited by 123 publications

References 23 publications

Effect of additional carbon source on biodegradation of linear alkylbenzene sulfonate by las-utilizing bacteria

Effect of additional carbon source on biodegradation of linear alkylbenzene sulfonate by las-utilizing bacteria

The alarmone (p)ppGpp mediates physiological‐responsive control at the σ54‐dependent Po promoter

Repression of the glucose-inducible outer-membrane protein OprB during utilization of aromatic compounds and organic acids in Pseudomonas putida CSV86

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The alarmone (p)ppGpp mediates physiological‐responsive control at the σ⁵⁴‐dependent Po promoter