Carbon Source Utilization Profiles for Microbial Communities from Hydrologically Distinct Zones in a Basalt Aquifer

Colwell, Frederick S.; Lehman, R. Michael

doi:10.1007/s002489900027

Cited by 18 publications

(8 citation statements)

References 37 publications

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“…Total counts of microbial cells in each of the well water samples determined by AODC were similar between wells and slightly greater than 10 3 cells mL À1 (Table 2). These values are approximately 10-fold lower than direct counts of microbial cells in the SRPA from past studies [40,44]. The lower values for the direct counts observed in the current study may be due to the isolation of the wells that were sampled compared to the other studies in which the wells were near existing facilities.…”

Section: Total Microbial Cells In Srpa Groundwatercontrasting

confidence: 72%

Diversity of methanotroph communities in a basalt aquifer

Newby

Reed

Petzke

et al. 2004

FEMS Microbiology Ecology

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Methanotrophic bacteria play an important role in global cycling of carbon and co-metabolism of contaminants. Methanotrophs from pristine regions of the Snake River Plain Aquifer (SRPA; Idaho, USA) were studied in order to gain insight into the native groundwater communities' genetic potential to carry out TCE co-metabolism. Wells were selected that were proximal to a TCE plume believed to be undergoing natural attenuation. Methane concentrations ranged from 1 to >1000 nM. Carbon isotope ratios and diversity data together suggest that the SRPA contains active communities of methanotrophs that oxidize microbially produced methane. Microorganisms removed from groundwater by filtration were used as inocula for enrichments or frozen immediately and DNA was subsequently extracted for molecular characterization. Primers that specifically target methanotroph 16S rRNA genes or genes that code for subunits of soluble or particulate methane monooxygenase, mmoX and pmoA, respectively, were used to characterize the indigenous methanotrophs via PCR, cloning, RFLP analysis, and sequencing. Type I methanotroph clones aligned with Methylomonas, Methylocaldum, and Methylobacter sequences and a distinct 16S rRNA phylogenetic lineage grouped near Methylobacter. The majority of clone sequences in type II methanotroph 16S rRNA, pmoA, and mmoX gene libraries grouped closely with sequences in the Methylocystis genus. A subset of the type II methanotroph clones from the aquifer had sequences that aligned most closely to Methylosinus trichosporium OB3b and Methylocystis spp., known TCE-co-metabolizing methanotrophs.

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Section: Total Microbial Cells In Srpa Groundwatercontrasting

confidence: 72%

Diversity of methanotroph communities in a basalt aquifer

Newby

Reed

Petzke

et al. 2004

FEMS Microbiology Ecology

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“…Analysis of the water samples prior to molasses treatment suggested that the total microbial cell numbers were consistent (ϳ10 4 cells/ml) with those measured previously in the lownutrient ESRPA environment (4,29); following molasses addition, the total cell numbers in the water phase increased by over an order of magnitude (Table 1). During the pretreatment phase, the AOB and AOA amoA genes were determined to be Ͻ10 and Ͻ100 copies each per ml of groundwater, respectively, together constituting less than 1% of the total microbial cell numbers.…”

Section: Resultsmentioning

confidence: 77%

“…Previous laboratory experiments indicated that molasses, an inexpensive and commonly used bioremediation amendment (14), was effective in increasing overall microbial populations, as well as total ureolytic activity (13,39). The ESRPA is a deep basalt aquifer and is considered oligotrophic (4,22,29); however, previous work has demonstrated the presence of ureolytic microbes in this environment (11,13). Erwin et al also reported evidence of AOB during the analysis of methane monooxygenase clone libraries from ESRPA samples (7), but in general, the structure and function of ammonia-oxidizing microbial communities (and especially AOA) in deep aquifers like the ESRPA have been relatively unexplored.…”

mentioning

confidence: 99%

Responses of Ammonia-Oxidizing Bacterial and Archaeal Populations to Organic Nitrogen Amendments in Low-Nutrient Groundwater

Reed

Smith

Francis

et al. 2010

Appl Environ Microbiol

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To evaluate the potential for organic nitrogen addition to stimulate the in situ growth of ammonia oxidizers during a field scale bioremediation trial, samples collected from the Eastern Snake River Plain Aquifer in Idaho before, during, and after the addition of molasses and urea were subjected to PCR analysis of ammonia monooxygenase subunit A (amoA) genes. Ammonia-oxidizing bacteria (AOB) and archaea (AOA) were present in all of the samples tested, with AOA amoA genes outnumbering AOB amoA genes in all of the samples. Following urea addition, nitrate levels rose and bacterial amoA copy numbers increased dramatically, suggesting that urea hydrolysis stimulated nitrification. Bacterial amoA diversity was limited to two Nitrosomonas phylotypes, whereas archaeal amoA analyses revealed 20 distinct operational taxonomic units, including several that were markedly different from all previously reported sequences. Results from this study demonstrate the likelihood of stimulating ammonia-oxidizing communities during field scale manipulation of groundwater conditions to promote urea hydrolysis.

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“…The ESRP is a semiarid high desert in southeastern Idaho in the United States. The stratigraphy of the ESRP is composed of multiple basalt ows with thin interbeds of sedimentary silts and clays and occasional sand and gravel layers (Anderson 1991;Colwell and Lehman 1997). Calcite precipitation is an integral reaction of the overall chemistry of the uncon ned aquifer (Wood and Low 1986;McLing 1994).…”

Section: Groundwater Samplesmentioning

confidence: 99%

Calcium Carbonate Precipitation by Ureolytic Subsurface Bacteria

Fujita¹,

Colwell²,

Smith³

et al. 2000

Geomicrobiology Journal

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Coprecipitation in carbonate minerals offers a means of slowing the transport of divalent radionuclides and contaminant metals (e.g., 90 Sr 2+ , UO 2+ 2 , Co 2+ ) in the subsurface. It may be possible to accelerate this process by stimulating the native microbial community to generate chemical conditions favoring carbonate precipitation. In a preliminary evaluation of this approach, we investigated the ability of ureolytic subsurface bacteria to produce alkaline conditions conducive to calcium carbonate precipitation. Groundwater samples from the Eastern Snake River Plain (ESRP) aquifer in Idaho were screened for urea-hydrolyzing microorganisms; three isolates were selected for further evaluation. Analysis of 16S rRNA gene sequences indicated that two of the ESRP isolates were of the genus Pseudomonas, and the other was a Variovorax sp. The speci c urease activities of the ESRP isolates appeared to be similar to each other but less than that

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Carbon Source Utilization Profiles for Microbial Communities from Hydrologically Distinct Zones in a Basalt Aquifer

Cited by 18 publications

References 37 publications

Diversity of methanotroph communities in a basalt aquifer

Diversity of methanotroph communities in a basalt aquifer

Responses of Ammonia-Oxidizing Bacterial and Archaeal Populations to Organic Nitrogen Amendments in Low-Nutrient Groundwater

Calcium Carbonate Precipitation by Ureolytic Subsurface Bacteria

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