Carbon sources-dependent carotenoid production in metabolically engineered Escherichia coli

Kim, Jongrae; Kong, Min; Lee, Sang Yup; Lee, Pyung Cheon

doi:10.1007/s11274-010-0408-5

Cited by 20 publications

(14 citation statements)

References 31 publications

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“…B). Like previous studies (Kim et al, 2010a;Yoon et al, 2009), this E. coli straindependency can be attributed to various factors and hardly to explain. Our results provide clues to understand the difference of isoprenoid production between E. coli strains and strain MG1655 is a suitable strain for ent-kaurene production.…”

Section: Comparison Of Ent-kaurene Production Among Various E Coli Ssupporting

confidence: 48%

Metabolic engineering of the Stevia rebaudiana ent-kaurene biosynthetic pathway in recombinant Escherichia coli

Kong

Kang

Kim

et al. 2015

Journal of Biotechnology

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Section: Comparison Of Ent-kaurene Production Among Various E Coli Ssupporting

confidence: 48%

Metabolic engineering of the Stevia rebaudiana ent-kaurene biosynthetic pathway in recombinant Escherichia coli

Kong

Kang

Kim

et al. 2015

Journal of Biotechnology

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“…Accordingly, for metabolic engineering of E. coli for isoprenoid production it is advisable not to use the arabinose-inducible promoters. As mentioned by other researchers, the exact mechanism of carbon source inhibition has yet to be elucidated (Kim et al 2010). In contrast, citrate was proved to be useful in increasing both the lycopene production and growth of all strains.…”

Section: Discussionmentioning

confidence: 94%

Type 2 IDI performs better than type 1 for improving lycopene production in metabolically engineered E. coli strains

Rad

Zahiri

Noghabi

et al. 2011

World J Microbiol Biotechnol

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In this study a comparison was made between type 1 and type 2 isopentenyl diphosphate isomerases (IDI) in improving lycopene production in Escherichia coli. The corresponding genes of Bacillus licheniformis and the host (i(Bl) and i(Ec), respectively) were expressed in lycopene producing E. coli strains by pTlyci(Bl) and pTlyci(Ec) plasmids, under the control of tac promoter. The results showed that the overexpression of i(Ec) improved the lycopene production from 33 ± 1 in E. coli Tlyc to 68 ± 3 mg/gDCW in E. coli Tlyci(Ec). In contrast, the expression of i(Bl) increased the lycopene production more efficiently up to 80 ± 9 mg/gDCW in E. coli Tlyci(Bl). The introduction of a heterologous mevalonate pathway to elevate the IPP abundance resulted in a lycopene production up to 132 ± 5 mg/gDCW with i(Ec) in E. coli Tlyci(Ec)-mev and 181 ± 9 mg/gDCW with i(Bl) in E. coli Tlyci(Bl)-mev, that is, 4 and 5.6 times respectively. When fructose, mannose, arabinose, and acetate were each used as an auxiliary substrate with glycerol, lycopene production was inhibited by different extents. Among auxiliary substrates tested, only citrate was an improving one for lycopene production in all strains with a maximum of 198 ± 3 mg/gDCW in E. coli Tlyci(Bl)-mev. It may be concluded that the type 2 IDI performs better than the type 1 in metabolic engineering attempts for isoprenoid production in E. coli. In addition, the metabolic engineering of citrate pathway seems a promising approach to have more isoprenoid accumulation in E. coli.

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“…1). The carotenoid biosynthetic pathways of Pantoea have been successfully used as basic pathways for many carotenoid studies (11)(12)(13). The pathway enzymes from different sources have occasionally shown unusual activities or substrate affinities in a heterologous host (14)(15)(16).…”

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confidence: 99%

Heterologous Carotenoid-Biosynthetic Enzymes: Functional Complementation and Effects on Carotenoid Profiles in Escherichia coli

Song

Kim

Choi

et al. 2013

Appl Environ Microbiol

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bA limited number of carotenoid pathway genes from microbial sources have been studied for analyzing the pathway complementation in the heterologous host Escherichia coli. In order to systematically investigate the functionality of carotenoid pathway enzymes in E. coli, the pathway genes of carotenogenic microorganisms (Brevibacterium linens, Corynebacterium glutamicum, Rhodobacter sphaeroides, Rhodobacter capsulatus, Rhodopirellula baltica, and Pantoea ananatis) were modified to form synthetic expression modules and then were complemented with Pantoea agglomerans pathway enzymes (CrtE, CrtB, CrtI, CrtY, and CrtZ). The carotenogenic pathway enzymes in the synthetic modules showed unusual activities when complemented with E. coli. For example, the expression of heterologous CrtEs of B. linens, C. glutamicum, and R. baltica influenced P. agglomerans CrtI to convert its substrate phytoene into a rare product-3,4,3=,4=-tetradehydrolycopene-along with lycopene, which was an expected product, indicating that CrtE, the first enzyme in the carotenoid biosynthesis pathway, can influence carotenoid profiles. In addition, CrtIs of R. sphaeroides and R. capsulatus converted phytoene into an unusual lycopene as well as into neurosporene. Thus, this study shows that the functional complementation of pathway enzymes from different sources is a useful methodology for diversifying biosynthesis as nature does.

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Carbon sources-dependent carotenoid production in metabolically engineered Escherichia coli

Cited by 20 publications

References 31 publications

Metabolic engineering of the Stevia rebaudiana ent-kaurene biosynthetic pathway in recombinant Escherichia coli

Metabolic engineering of the Stevia rebaudiana ent-kaurene biosynthetic pathway in recombinant Escherichia coli

Type 2 IDI performs better than type 1 for improving lycopene production in metabolically engineered E. coli strains

Heterologous Carotenoid-Biosynthetic Enzymes: Functional Complementation and Effects on Carotenoid Profiles in Escherichia coli

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