Carbon use, nitrogen use, and isotopic fractionation of ectomycorrhizal and saprotrophic fungi in natural abundance and 13C-labelled cultures

Hobbie, Erik A.; Sánchez, Fernando Sánchez; Rygiewicz, Paul T.

doi:10.1017/s0953756204000590

Cited by 32 publications

(28 citation statements)

References 27 publications

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“…For example, Rossmann et al [28] found that there was a non-random distribution of C isotopes in natural glucose, with a relative enrichment in position four (C-4) and a depletion in position 6 (C-6) of the C atoms within glucose. It has been shown [25] that fungi generally incorporate C-6 preferentially over C-4, and this would go some way to explaining our results.…”

Section: Discussionsupporting

confidence: 67%

“…The level of enrichment obtained showed that the fungi were utilising a proportion of the C and N present in the agar gel, and this was in line with previous studies which found saprotrophic fungi obtaining up to 45% of their C from the agar substrate. [25] The results show that the growth rate of the fungal isolate was only affected when it was grown on the dual enriched media, with the fungi able to compensate and maintain the same growth rate (in comparison with natural abundance) when on a singly enriched medium. This may be explained by the different proportions of agar gel utilised on the singly enriched medium from those on the dually enriched medium.…”

Section: Discussionmentioning

confidence: 91%

“…Studies have shown that saprotrophic fungi can obtain up to 45% of their C from the agar gel. [25] If this was the case here it would have had the effect of diluting the overall enrichment. In order for the samples to be diluted to an atom% ratio within the thresholds of the mass spectrometer, a reference flour with a known C content of 41.96% and 1.0822 atom% 13 C was used to dilute the sample according to the following equations: [26] …”

Section: Enrichment Measurements Of Fungal Isolatesmentioning

confidence: 91%

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Differential growth of the fungus Absidia cylindrospora on ¹³C/¹⁵N‐labelled media

Crotty

Blackshaw

Murray

2011

Rapid Comm Mass Spectrometry

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Many studies utilise enrichment of stable isotopes as tracers to follow the interactions occurring within soil food webs and methods have been developed to enrich bacteria, soil fauna and plant litter, Here for the first time we attempt to enrich a soil fungus to 99 atom% with (13)C and (15)N stable isotopes. In this study our objectives were to (a) assess whether the saprotrophic zygomycete fungus Absidia cylindrospora could grow on a medium enriched to 99 atom% with (13)C-glucose and (15)N-ammonium chloride, (b) to determine the level of enrichment obtained, and (c) to examine the change in growth rate of this fungus while it was growing on the dually enriched medium. To achieve this, the fungus was grown on agar enriched with (13)C and (15)N to 99 atom% and its growth rate monitored. The results showed that A. cylindrospora would grow on the highly labelled growth medium, but that its rate of growth was affected compared with the rate on either natural abundance media or media highly enriched with a single isotope ((13)C or (15)N). The implications of these results is that although the fungus is able to utilise these heavier isotopes, the biochemical processes involved in growth are affected, and consideration should be given to these differences when using stable isotope tracers in, for example, soil food web studies.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 91%

Section: Enrichment Measurements Of Fungal Isolatesmentioning

confidence: 91%

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Differential growth of the fungus Absidia cylindrospora on ¹³C/¹⁵N‐labelled media

Crotty

Blackshaw

Murray

2011

Rapid Comm Mass Spectrometry

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“…The presence of saprotrophic fungi on maize residues may have caused this reduction in δ 13 C and δ 15 N values. A fractionation of δ 13 C and δ 15 N values has been repeatedly observed in saprotrophic fungal biomass (Hobbie et al 2004). This fractionation was also caused by ecto-mycorrhizal and even by AM fungi, additionally affecting the δ 15 N values of their host plants .…”

Section: Microbial Use Of Maize Residuesmentioning

confidence: 74%

Impact of pea growth and arbuscular mycorrhizal fungi on the decomposition of 15N-labeled maize residues

Jannoura

Kleikamp²,

Dyckmans

et al. 2011

Biol Fertil Soils

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A pot experiment was carried out (1) to compare C and N yield of different plant parts, nutrient concentrations, and root colonization between the non-mycorrhizal mutant P2 (myc − ) and the symbiotic isoline Frisson (myc + ), (2) to investigate the effects of arbuscular mycorrhizal fungi and growing pea plants on microbial decomposition of 15 Nlabeled maize residues, and (3) to follow the distribution of the added substrate over different soil fractions, such as particulate organic matter, soil microbial biomass, and microbial residues. Yields of C in straw, grain, and roots of myc + peas were significantly higher by 27%, 11%, and 92%, respectively, compared with those of myc − peas. The δ 13 C values in the different plant parts were significantly higher in myc + than in myc − tissue with and without maize. Application of labeled maize residues generally resulted in 15 N enrichment of pea plants. At the end of the experiment, the ergosterol concentration in roots of mature peas did not differ between the two isolines, indicating similar colonization by saprotrophic fungi. The decomposition of added maize residues was significantly reduced by the myc − peas, but especially by myc + peas. The formation of microbial residue C was increased and that of microbial residue N was reduced in the presence of plants. The insufficient N supply to soil microorganisms reduced decomposition of maize residues in the presence of peas, especially myc + peas.

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“…Monosaccharaides, especially the basic ubiquitous substrate glucose (Macura and Kubatova, 1973), are classical substrates for metabolic tracing analysis (Hobbie et al, 2004;Scandellari et al, 2009), as they are spread throughout all metabolic pathways and can be found in each product. However, recent studies tracing citric acid cycle activity mainly chose pyruvate or the pyruvate-precursor alanine as a metabolic tracer (Wegener et al, 2010;Dijkstra et al, 2011bDijkstra et al, , 2011cApostel et al, 2013;Dippold and Kuzyakov, 2013).…”

Section: Metabolic Tracing By Position-specific Labeling Of Monosacchmentioning

confidence: 99%

Biochemistry of hexose and pentose transformations in soil analyzed by position-specific labeling and 13C-PLFA

Apostel

Dippold

Kuzyakov

2015

Soil Biology and Biochemistry

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Carbon use, nitrogen use, and isotopic fractionation of ectomycorrhizal and saprotrophic fungi in natural abundance and 13C-labelled cultures

Cited by 32 publications

References 27 publications

Differential growth of the fungus Absidia cylindrospora on ¹³C/¹⁵N‐labelled media

Differential growth of the fungus Absidia cylindrospora on ¹³C/¹⁵N‐labelled media

Impact of pea growth and arbuscular mycorrhizal fungi on the decomposition of 15N-labeled maize residues

Biochemistry of hexose and pentose transformations in soil analyzed by position-specific labeling and 13C-PLFA

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Carbon use, nitrogen use, and isotopic fractionation of ectomycorrhizal and saprotrophic fungi in natural abundance and 13C-labelled cultures

Cited by 32 publications

References 27 publications

Differential growth of the fungus Absidia cylindrospora on 13C/15N‐labelled media

Differential growth of the fungus Absidia cylindrospora on 13C/15N‐labelled media

Impact of pea growth and arbuscular mycorrhizal fungi on the decomposition of 15N-labeled maize residues

Biochemistry of hexose and pentose transformations in soil analyzed by position-specific labeling and 13C-PLFA

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Differential growth of the fungus Absidia cylindrospora on ¹³C/¹⁵N‐labelled media

Differential growth of the fungus Absidia cylindrospora on ¹³C/¹⁵N‐labelled media