Carbonic Anhydrase in Chlamydomonas reinhardtii II. Requirements for Carbonic Anhydrase Induction

Spencer, Kenneth G.; Kimpel, Donald L.; Fisher, Maureen L.; Togasaki, Robert K.; Miyachi, Shigetoh

doi:10.1093/pcp/24.2.301

Cited by 50 publications

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“…It was shown that in addition to a lowering of CO2 concentration, light is also required for CA induction in C. reinhardtii (23,24). We have shown that light exerts its control on CA activity of C. reinhardtii through photosynthesis as well as through a photosynthesis-independent blue light-requiring process (9,10).…”

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confidence: 77%

“…It was previously shown that addition of the translation inhibitor cycloheximide immediately stopped CA induction in C. reinhardtii (7,24,32). This result indicated that CA is synthesized on cytoplasmic 80S ribosomes and is, therefore, encoded in the nuclear genome.…”

Section: Effects Of Transcription Inhibitors On Ca Mrna Accumulation mentioning

confidence: 94%

“…The periplasmic CA has been shown to be important in the supply to the cells of free CO2 from the dehydration of HCO3- (1,25), and has been identified to be a glycoprotein with a subunit molecular mass estimated at 35 to 37 kD (6,29,32). The induction of CA activity under low-CO2 conditions was shown to be inhibited by the protein translation inhibitor, cycloheximide (22,24,32), suggesting that the enzyme was synthesized de novo when the CO2 concentration was lowered. Subsequently, evidence was provided confirming that the increase in CA activity paralleled the increase in CA protein (32).…”

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confidence: 99%

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Light-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii

Dionisio-Sese¹,

Fukuzawa²,

Miyachi³

1990

Plant Physiol.

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confidence: 77%

Section: Effects Of Transcription Inhibitors On Ca Mrna Accumulation mentioning

confidence: 94%

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confidence: 99%

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Light-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii

Dionisio-Sese¹,

Fukuzawa²,

Miyachi³

1990

Plant Physiol.

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“…Blue light caused a twofold increase of CA activity in Chlamydomonas reinhardtii (11,35). Spencer et al (35) demonstrated that Chlamydomonas reinhardtii CA activity could not be induced without light, even at low CO2 concentration.…”

Section: Discussionmentioning

confidence: 99%

Extra and intracelular activities of carbonic anhydrase of the marine microalga Tetraselmis gracilis (Chlorophyta)

Rigobello-Masini¹,

Aidar²,

Masini³

2003

Braz. J. Microbiol.

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The activities of extra and intracellular carbonic anhydrases (CA) were studied in the microalgae Tetraselmis gracilis (Kylin) Butcher (Chlorophyta, Prasinophyceae) growing in laboratory cultivation. During ten days of batch cultivation, daily determinations of pH, cell number, enzymatic activity, and total dissolved inorganic carbon (DIC), as well as its main species, CO2 and HCO3 -, were performed. Enzymatic activity increased as the growing cell population depleted inorganic carbon from the medium. Carbon dioxide concentration decreased quickly, especially in the third day of cultivation, when a significant increase of the intracellular enzymatic activity was observed. Bicarbonate concentration had its largest decrease in the cultivation medium in the fourth day, when the activity of the extracellular enzyme had its largest increase, suggesting its use by the alga through CA activity. After the fourth cultivation day, half of the cultures were aerated with CO2-free atmospheric air, which caused an increase in the total and external activity of the enzyme, although, in this condition, the stationary growth phase began earlier than in cultures aerated with atmospheric air. The pH of the media was measured daily, increasing from the first to the fourth day, and remaining almost constant until the end of the cultivation. Algal material transferred to the dark lost all enzymatic activity.

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“…A carbonic anhydrase located in the periplasmic space of the alga has been identified as part of the CO2 concentrating system (6, 1 1, 31). This protein is made only when the cells are grown in the light with low concentrations of CO2 (8, 27,29,30). The existence of other components of the CO2 concentrating system has been inferred from studies of C. reinhardtii mutants (23)(24)(25), Ci uptake measurements (21), and from 35S-labeling studies of cells that are adapting to low CO2 conditions (13).…”

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confidence: 99%

Isolation and Characterization of a Mutant of Chlamydomonas reinhardtii Deficient in the CO₂ Concentrating Mechanism

Moroney¹,

Husic²,

Tolbert³

et al. 1989

Plant Physiol.

123

100

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A Chiamydomonas reinhardtii mutant has been isolated that cannot grow photoautotrophically on low CO2 concentrations but can grow on elevated CO2. In a test cross, the high C02-requirement for growth showed a 2:2 segregation. This mutant, designated CIA-5, had a phenotype similar to previously identified mutants that were defective in some aspect of CO2 accumulation. Unlike previously isolated mutants, CIA-5 did not have detectable levels of the periplasmic carbonic anhydrase, an inducible protein that participates in the acquisition of CO2 by C. reinhardtii. CIA-5 also did not accumulate inorganic carbon to levels higher than could be accounted for by diffusion. This mutant strain did not synthesize any of the four polypeptides preferentially made by wild type C. reinhardtii when switched from an environment containing elevated CO2 levels to an environment low in CO2. It is concluded that this mutant fails to induce the CO2 concentrating system and is incapable of adapting to low CO2 conditions. Chlamydomonas reinhardtii, like other unicellular green algae, has the capacity to adapt to varying CO2 concentrations in the environment (1-3, 5). When grown on elevated levels of CO2 (5% (v/v) in air), C. reinhardtii has a relatively low affinity for C12 and exhibits high rates of photorespiration when placed in a low CO2/high O2 environment (2,16,19,26). However, if C. reinhardtii remains exposed to low levels of CO2 (ambient CO2 levels), it adapts to these conditions by inducing a CO2 concentrating mechanism (2). This CO2 concentrating mechanism is thought to increase the CO2 at the

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Carbonic Anhydrase in Chlamydomonas reinhardtii II. Requirements for Carbonic Anhydrase Induction

Cited by 50 publications

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Light-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii

Light-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii

Extra and intracelular activities of carbonic anhydrase of the marine microalga Tetraselmis gracilis (Chlorophyta)

Isolation and Characterization of a Mutant of Chlamydomonas reinhardtii Deficient in the CO₂ Concentrating Mechanism

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Carbonic Anhydrase in Chlamydomonas reinhardtii II. Requirements for Carbonic Anhydrase Induction

Cited by 50 publications

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Light-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii

Light-Induced Carbonic Anhydrase Expression in Chlamydomonas reinhardtii

Extra and intracelular activities of carbonic anhydrase of the marine microalga Tetraselmis gracilis (Chlorophyta)

Isolation and Characterization of a Mutant of Chlamydomonas reinhardtii Deficient in the CO2 Concentrating Mechanism

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Isolation and Characterization of a Mutant of Chlamydomonas reinhardtii Deficient in the CO₂ Concentrating Mechanism