Carbonic Anhydrase in the Marine Alga Ulva pertusa

Abstract

The preparation of stable samples containing carbonic anhydrase from Ulva pertusa was achieved by using artificial sea water and 10 mM cysteine solution adjusted to pH 8.2 instead of 10 mM cysteine solution alone. When the sample was stored at 0°C in darkness the enzyme activity was retained for about 24 h. In contrast to the results obtained with land plants in previous works a remarkable inhibitory effect of low concentrations of sulfanilamide and Diamox (2‐acetylamino‐1,3,4‐thiadiazole‐5‐sulfonamide) on the activity of the enzyme was observed.

Effects of Acetazolamide (Diamox), Ethoxzolamide and High Levels of CO2 on Carbonic Anhydrase, Photosystem Activity, and Oxygen Evolution in Euglena gracilis

“…Carbonic anhydrase (carbonate hydrolase, EC 4.2.1.1) has been implicated as a regulatory enzyme in several photosynthetic systems. For example, COj fixation is inhibited in Ulva pertusa (Ikemori and Nishida 1968) and spinach cloroplast preparations (Everson 1970) by several inhibitors of this enzyme. When CO 2 tensions are low in cultures of Chlamydomonas reinhardii (Nelson et al 1969) or Chlorella pyrenoidosa Reed 1971, Graham et al 1971), the rate of photosynthesis shows a direct correlation with the carbonic anhydrase activity.…”

Carbonic anhydrase levels and internal lacunar CO/sub 2/ concentrations in aquatic macrophytes

“…Carbonic anhydrase is present in virtually all organisms: in plants, animals, and bacteria (Lindskog et al, 1971). In the plant kingdom, carbonic anhydrase is present in both freshwater and marine algae (Bowes, 1969;Graham and Smillie, 1976;Ikemori and Nishida, 1968;Ingle and Colman, 1975;Lichtfield and Hood, 1964), in bryophytes (Brown and Eyster, 1955;Steemann Nielsen and Kristiansen, 1949), in pteridophytes and gymnosperms (Graham et al, 1974), and in monocotyledonous and dicotyledonous vascular plants (Atkins et al, 1972a, b;Chen et al, 1970;Everson and Slack, 1968). The enzyme is present only in leaves and is not present in roots, although the enzyme is present in the root nodules of leguminous species (Atkins, 1974).…”

Carbonic Anhydrase in Marine Algae

“…Plant carbonic anlhydrase is thought to be only wveaklx affected, if at all, by sulfonamides (5,9) although the literature shows s,ome variability (1,11,13,19,24). It has been found by some workers that free sulfhydryl groups are necessarv for expression of the plant enzyme or for its enhancement (1,23) but variability in this regard has ailso been reported (15,24).…”