Carbonic anhydrase IX in oligodendroglial brain tumors

Järvelä, Sally; Parkkila, Seppo; Bragge, Helena; Kähkönen, Marketta; Parkkila, Anna‐Kaisa; Soini, Ylermi; Pastoreková, Silvia; Pastorek, Jaromı́r; Haapasalo, Hannu

doi:10.1186/1471-2407-8-1

Cited by 182 publications

(127 citation statements)

References 43 publications

(36 reference statements)

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“…A relationship between CA IX and PFS or OS has been identified in other gliomas [22,23]. Our results may be related to our use of a monospecific CA IX antibody that did not cross react with other carbonic anhydrase subtypes found in benign tissues, such as blood vessels [22,23].…”

Section: Discussioncontrasting

confidence: 38%

Prognostic marker analysis in pediatric intracranial ependymomas

et al. 2015

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Histologic grading methods dependent upon H&E staining review have not been shown to reliably predict survival in children with intracranial ependymomas due to the subjectivity of the analytical methods. We hypothesized that the immunohistochemical detection of MIB-1, Tenascin C, CD34, VEGF, and CA IX may represent objective markers of post-operative survival (Progression Free and Overall Survival; PFS, OS) in these patients. Intracranial ependymomas from patients aged 22 years or less were studied. The original histologic grade was recorded, H&E sections were reviewed for vascular proliferation status, and immunohistochemistry was used to determine MIB-1, Tenascin C, CD34, VEGF, and CA IX status. Based upon the World Health Organization (WHO) grading system, 3 Grade I, 18 Grade II and 9 Grade III ependymomas were studied. Median follow-up time was 9.0 years; median PFS was, 6.1 years. Original WHO grade did not correlate with PFS or OS. Peri-necrotic CA IX localization correlated with PFS (Log rank = 0.0181) and OS (Log rank p = 0.0015). All patients with a CA IX ≤ 5 % total area localization were alive at last follow-up. Perinecrotic CA IX staining was also associated with vascular proliferation (p = 0.006), though not with VEGF expression score. MIB-1 labeling index (LI) correlated with OS (HR 1.06, 95 % CI 1.01, 1.12) and PFS (HR 1.08, 95 % CI 1.02, 1.14). MIB-1 LI and perinecrotic CA IX individually correlated with PFS. The effect of perinecrotic CA IX remained when grade was added to a Cox model predicting PFS. Immunodetection of CA IX and MIB-1 expression are predictive biomarkers for survival in children with posterior fossa ependymomas. These markers represent objective indicators of survival that supplement H&E grading alone.

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Section: Discussioncontrasting

confidence: 38%

Prognostic marker analysis in pediatric intracranial ependymomas

et al. 2015

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“…This includes non-small-cell lung cancer (studied by Swinson et al (2003) and Giatromanolaki et al (2001)), bladder cancer (Hoskin et al, 2003), invasive breast cancer (Chia et al, 2001;Brennan et al, 2006) and oligodendroglioma (Järvelä et al, 2008). Interestingly, in renal cancer, low CA IX expression and absence of VHL mutation were related to a more advanced tumour and unfavourable outcome (Patard et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Expression of carbonic anhydrase IX suggests poor outcome in rectal cancer

et al. 2009

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The aim of the study is to assess the value of carbonic anhydrase isozyme IX (CA IX) expression as a predictor of disease-free survival (DFS) and disease-specific survival (DSS) in rectal cancer treated by preoperative radio-or chemoradiotherapy or surgery only. Archival tumour samples from 166 patients were analysed for CA IX expression by three different evaluations: positive/negative, proportion of positivity and staining intensity. The results of immunohistochemical analysis were confirmed by demonstrating CA IX protein in western blotting analysis. Forty-four percent of the operative samples were CA IX positive, of these 34% had weak and 66% moderate/strong staining intensity. In univariate survival analysis, intensity of CA IX expression was a predictor of DFS (P ¼ 0.003) and DSS (P ¼ 0.034), both being markedly longer in tumours with negative or weakly positive staining. In multivariate Cox model, number of metastatic lymph nodes and CA IX intensity were the only independent predictors of DFS. Carbonic anhydrase isozyme IX intensity was the only independent predictor of DSS, with HR ¼ 9.2 for dying of disease with moderate-intense CA IX expression as compared with CA IX-negative/weak cases. Negative/weak CA IX staining intensity is an independent predictor of longer DFS and DSS in rectal cancer.

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“…The reference genes (β‐actin, glyceraldehyde‐3‐phosphate dehydrogenase, hydroxymethylbilane synthase, and subunit A of the succinate dehydrogenase complex) were selected based on previously published literature 26 and designed based on published sequences 25. Specific primers for the target genes were designed using the publicly available web‐based Primer3 program (http://frodo.wi.mit.edu/primer3).…”

Section: Methodsmentioning

confidence: 99%

The molecular mechanism of the cholesterol‐lowering effect of dill and kale: The influence of the food matrix components

et al. 2016

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Foods are complex matrices containing many different compounds, all of which contribute to the overall effect of the food itself, although they have different mechanisms of action. While evaluating the effect of bioactive compounds, it is important to consider that the use of a single compound can hide the effects of the other molecules that can act synergistically or antagonistically in the same food. The aim of the present study was to evaluate the influence of food matrix components by comparing two edible plants (dill and kale) with cholesterol‐lowering potential and similar contents of their most representative bioactive, quercetin. The molecular effects of the extracts were evaluated in HepG2 cells by measuring the expression of sterol‐regulatory element‐binding proteins (SREBPs), 3‐hydroxy‐3‐methylglutaryl‐CoA reductase (HMGCR) and low density lipoprotein receptor (LDLR) at the mRNA and protein level. The results reported here show that both extracts reduced the cellular cholesterol level with a similar trend and magnitude. It is conceivable that the slightly different results are due to the diverse composition of minor bioactive compounds, indicating that only by considering food as a whole is it possible to understand the complex relationship between food, nutrition, and health in a foodomics vision.

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Carbonic anhydrase IX in oligodendroglial brain tumors

Abstract: Background: Carbonic anhydrase IX is a hypoxia-induced enzyme that has many biologically important functions, including its role in cell adhesion and invasion.

Cited by 182 publications

References 43 publications

Prognostic marker analysis in pediatric intracranial ependymomas

Prognostic marker analysis in pediatric intracranial ependymomas

Expression of carbonic anhydrase IX suggests poor outcome in rectal cancer

The molecular mechanism of the cholesterol‐lowering effect of dill and kale: The influence of the food matrix components

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