Carbonyl Cyanide Phenylhydrazones as Probes of the Anionic Activator Site of the Human Erythrocyte Glutathione Adduct Transport ATPase

DeLuca, Donald C.; Hinds, Trenton; Winter, Charles G.

doi:10.1006/abbi.1997.0120

Cited by 2 publications

(1 citation statement)

References 21 publications

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“…These studies for the first time demonstrated that the erythrocyte GS-E transporter, DNP-SG ATPase, could mediate ATP-dependent transport of weakly cationic antitumor agents as well as the organic anions such as DNP-SG. The stimulation of erythrocyte membrane ATPase activity by the two classical uncouplers of oxidative phosphorylation, 2,4-dinitrophenol and carbonylcyanide p-fluoromethoxyphenylhydrazone, has been reported [53,54]. Both these compounds competed with DNP-SG for the activation of GS-E ATPase activity suggesting that small anionic, lipophilic compounds may also be transported by this system.…”

Section: Dnp-sg Atpase: a Multidrug Transportermentioning

confidence: 99%

Transport of glutathione-conjugates in human erythrocytes.

Sharma

Awasthi

Zimniak³

et al. 2000

Acta Biochim Pol

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The last step of detoxification of both endogenous and environmental toxicants is typically a conjugation that produces a bulky hydrophilic molecule. The excretion of such conjugates out of cells is of sufficient biological importance to have led to the evolution of ATP-driven export pumps for this purpose. The substrate specificity of such transporters is broad, and in some cases it has been shown to include not only anionic conjugates but also neutral or weakly cationic drugs. In the present article, we review the molecular identity, functional and structural characteristics of these pumps, mainly on the example of human erythrocytes, and discuss their physiological role in detoxification and in the multidrug resistance phenotype of cancer cells.

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Section: Dnp-sg Atpase: a Multidrug Transportermentioning

confidence: 99%

Transport of glutathione-conjugates in human erythrocytes.

Sharma

Awasthi

Zimniak³

et al. 2000

Acta Biochim Pol

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Effects of DL-alpha-lipoic acid on peripheral nerve conduction, blood flow, energy metabolism, and oxidative stress in experimental diabetic neuropathy.

et al. 2000

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Experimental diabetic peripheral neuropathy (DPN) is marked by impaired nerve conduction velocity (NCV), reduced nerve blood flow (NBF), and a variety of metabolic abnormalities in peripheral nerve that have been variously ascribed to hyperglycemia, abnormal fatty acid metabolism, ischemic hypoxia, and/or oxidative stress. Some investigators propose that NCV slowing in experimental DPN can be explained entirely on the basis of nerve energy depletion secondary to reduced NBF. This article reports highly selective effects of administration of the antioxidant DL-␣-lipoic acid (LA) to streptozotocin-injected diabetic rats. LA improved digital sensory but not sciatic-tibial motor NCV, corrected endoneurial nutritive but not composite NBF, increased the mitochondrial oxidative state without correcting nerve energy depletion, and enhanced the accumulation of polyol pathway intermediates without worsening myo-inositol or taurine depletion. These studies implicate oxidative stress as an important pathophysiological factor in experimental DPN. They reveal complex interrelationships among nerve perfusion, energy metabolism, osmolyte content, conduction velocity, and oxidative stress that may reflect the heterogeneous and compartmentalized composition of peripheral nerve.

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Carbonyl Cyanide Phenylhydrazones as Probes of the Anionic Activator Site of the Human Erythrocyte Glutathione Adduct Transport ATPase

Cited by 2 publications

References 21 publications

Transport of glutathione-conjugates in human erythrocytes.

Transport of glutathione-conjugates in human erythrocytes.

Effects of DL-alpha-lipoic acid on peripheral nerve conduction, blood flow, energy metabolism, and oxidative stress in experimental diabetic neuropathy.

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