Carbonylation of the cytoskeletal protein actin leads to aggregate formation

Castro, José Pedro; Ott, Christiane; Jung, Tobias; Grune, Tilman; Almeida, Henrique

doi:10.1016/j.freeradbiomed.2012.06.005

Cited by 54 publications

(50 citation statements)

References 45 publications

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“…irreversible non-enzymatic modification, that can lead to protein dysfunction and aggregation (Nyström, 2005;Castro et al, 2012). This protein modification is regarded as a severe marker of protein oxidation and dysfunction, shown to accumulate during aging (and age-related diseases) and in chronic diseases such as chronic renal failure or chronic lung disease (Levine and Stadtman, 2001;Dalle-Donne et al, 2003).…”

Section: Consequences Of Ros Imbalance For Adipocyte Functionalitymentioning

confidence: 99%

The two faces of reactive oxygen species (ROS) in adipocyte function and dysfunction

Castro

Grune

Speckmann

2016

Biological Chemistry

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125

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White adipose tissue (WAT) is actively involved in the regulation of whole-body energy homeostasis via storage/release of lipids and adipokine secretion. Current research links WAT dysfunction to the development of metabolic syndrome (MetS) and type 2 diabetes (T2D). The expansion of WAT during oversupply of nutrients prevents ectopic fat accumulation and requires proper preadipocyte-to-adipocyte differentiation. An assumed link between excess levels of reactive oxygen species (ROS), WAT dysfunction and T2D has been discussed controversially. While oxidative stress conditions have conclusively been detected in WAT of T2D patients and related animal models, clinical trials with antioxidants failed to prevent T2D or to improve glucose homeostasis. Furthermore, animal studies yielded inconsistent results regarding the role of oxidative stress in the development of diabetes. Here, we discuss the contribution of ROS to the (patho)physiology of adipocyte function and differentiation, with particular emphasis on sources and nutritional modulators of adipocyte ROS and their functions in signaling mechanisms controlling adipogenesis and functions of mature fat cells. We propose a concept of ROS balance that is required for normal functioning of WAT. We explain how both excessive and diminished levels of ROS, e.g. resulting from over supplementation with antioxidants, contribute to WAT dysfunction and subsequently insulin resistance.

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Section: Consequences Of Ros Imbalance For Adipocyte Functionalitymentioning

confidence: 99%

The two faces of reactive oxygen species (ROS) in adipocyte function and dysfunction

Castro

Grune

Speckmann

2016

Biological Chemistry

Self Cite

125

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“…In addition, actin carbonylated in excess can undergo aggregation and inhibit the proteolytic activity of the proteasome (Castro et al 2012). It is possible that changes in ACT2 expression associated with carbonyl aggregates could inhibit the proteasome degradation pathway and, consequently, decrease the recycling of ubiquitin by lowering the proteosomal activity.…”

Section: Protein Identificationmentioning

confidence: 99%

Changes in protein expression of pacific oyster Crassostrea gigas exposed in situ to urban sewage

Flores-Nunes

Gomes

Moraes

et al. 2014

Environ Sci Pollut Res

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The composition and concentration of substances in urban effluents are complex and difficult to measure. These contaminants elicit biological responses in the exposed organisms. Proteomic analysis is a powerful tool in environmental toxicology by evidencing alterations in protein expression due to exposure to contaminants and by providing a useful framework for the development of new potential biomarkers. The aim of this study was to determine changes in protein expression signatures (PES) in the digestive gland of oysters Crassostrea gigas transplanted to two farming areas (LIS and RIB) and to one area contaminated by sanitary sewage (BUC) after 14 days of exposure. This species is one of the most cultivated molluscs in the world. The identified proteins are related to the cytoskeleton (CKAP5 and ACT2), ubiquitination pathway conjugation (UBE3C), G protein-coupled receptor and signal transduction (SVEP1), and cell cycle/division (CCNB3). CKAP5 showed higher expression in oysters kept at BUC in comparison with those kept at the farming areas, while ACT2, UBE3C, SVEP1, and CCNB3 were suppressed. The results suggest that these changes might lead to DNA damage, apoptosis, and interference with the immune system in oyster C. gigas exposed to sewage and give initial information on PES of C. gigas exposed to sanitary sewage, which can subsequently be useful in the development of more sensitive tools for biomonitoring coastal areas, particularly those devoted mainly to oyster farming activities.

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“…This PTM, as well as protein homocysteinylation, can commit proteins to degradation by the ubiquitin-proteasome system (UPS) (Grimm et al, 2012;Jakubowski and Glowacki, 2011). Alternatively, modified proteins can aggregate, contributing to cytoskeleton disorganization and further cell functional impairment (Castro et al, 2012). Our earlier results also demonstrated that the proteome of myocardium of rat pups was dramatically altered after maternal MD deprivation (Martinez et al, 2013).…”

mentioning

confidence: 87%

Methyl donor deficiency in H9c2 cardiomyoblasts induces ER stress as an important part of the proteome response

Martinez

Deval

Jousse

et al. 2015

The International Journal of Biochemistry & Cell Biology

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Carbonylation of the cytoskeletal protein actin leads to aggregate formation

Cited by 54 publications

References 45 publications

The two faces of reactive oxygen species (ROS) in adipocyte function and dysfunction

The two faces of reactive oxygen species (ROS) in adipocyte function and dysfunction

Changes in protein expression of pacific oyster Crassostrea gigas exposed in situ to urban sewage

Methyl donor deficiency in H9c2 cardiomyoblasts induces ER stress as an important part of the proteome response

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