Carboxydothermus hydrogenoformans gen. nov., sp. nov., a CO-utilizing Thermophilic Anaerobic Bacterium from Hydrothermal Environments of Kunashir Island

Svetlichny, V. A.; Sokolova, T. G.; Gerhardt, Matthias; Ringpfeil, M.; Kostrikina, N. A.; Zavarzin, G. A.

doi:10.1016/s0723-2020(11)80377-2

Cited by 161 publications

(151 citation statements)

References 21 publications

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“…With the exception of C. ferrireducens (Henstra & Stams, 2004;Slobodkin et al, 2006), all members of the genus Carboxydothermus are carboxydotrophic hydrogenogens (Table 1). Consistent with the metabolic reaction, the molar amounts of CO consumption and H 2 production during growth of C. hydrogenoformans, C. siderophilus and C. islandicus are almost equivalent (Svetlichny et al, 1991;Slepova et al, 2009;Novikov et al, 2011). However, strain Ug1 T produced a smaller amount of H 2 than expected, approximately 69 % of the CO uptake (see Fig.…”

supporting

confidence: 73%

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Carboxydothermus pertinax sp. nov., a thermophilic, hydrogenogenic, Fe(III)-reducing, sulfur-reducing carboxydotrophic bacterium from an acidic hot spring

Yoneda

Yoshida

Kawaichi

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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A novel anaerobic, Fe(III)-reducing, hydrogenogenic, carboxydotrophic bacterium, designated strain Ug1 T , was isolated from a volcanic acidic hot spring in southern Kyushu Island, Japan. Cells of the isolate were rod-shaped (1.0-3.0 mm long) and motile due to peritrichous flagella. Strain Ug1 T grew chemolithoautotrophically on CO (100 % in the gas phase) with reduction of ferric citrate, amorphous iron (III) oxide, 9,10-anthraquinone 2,6-disulfonate, thiosulfate or elemental sulfur. No carboxydotrophic growth occurred with sulfate, sulfite, nitrate or fumarate as electron acceptor. During growth on CO, H 2 and CO 2 were produced. Growth occurred on molecular hydrogen as an energy source and carbon dioxide as a sole carbon source. Growth was observed on various organic compounds under an N 2 atmosphere with the reduction of ferric iron. The temperature range for carboxydotrophic growth was 50-70 6C, with an optimum at 65 6C. The pH 25 6C range for growth was 4.6-8.6, with an optimum between 6.0 and 6.5. The doubling time under optimum conditions using CO with ferric citrate was 1.5 h. The DNA G+C content was 42.2 mol%. Analysis of 16S rRNA gene sequences demonstrated that this strain belongs to the thermophilic carboxydotrophic bacterial genus Carboxydothermus, with sequence similarities of 94.1-96.6 % to members of this genus. The isolate can be distinguished from other members of the genus Carboxydothermus by its ability to grow with elemental sulfur or thiosulfate coupled to CO oxidation. On the basis of phylogenetic analysis and unique physiological features, the isolate represents a novel species of the genus Carboxydothermus for which the name Carboxydothermus pertinax sp. nov. is proposed; the type strain of the novel species is Ug1 T (5DSM 23698 T 5NBRC 107576 T ).

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supporting

confidence: 73%

“…Strains: 1, Ug1 T ; 2, C. hydrogenoformans Z-2901 T ; 3, C. ferrireducens JW/AS-Y7 T ; 4, C. siderophilus 1315 T ; and 5, C. islandicus SET IS-9 T . Data for reference strains are taken from Svetlichny et al (1991), Henstra & Stams (2004), Slobodkin et al (1997Slobodkin et al ( , 2006, Slepova et al (2009) …”

mentioning

confidence: 99%

Carboxydothermus pertinax sp. nov., a thermophilic, hydrogenogenic, Fe(III)-reducing, sulfur-reducing carboxydotrophic bacterium from an acidic hot spring

Yoneda

Yoshida

Kawaichi

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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“…The reference strains used in this study were Carboxydothermus hydrogenoformans Z-2901 T (DSM 6008 T ) (Svetlichny et al, 1991), Carboxydocella thermautotrophica 41 T (DSM 12326 T ) (Sokolova et al, 2002) and Caldanaerobacter subterraneus subsp. pacificus JM T (DSM 12653 T ) (Sokolova et al, 2001;Fardeau et al, 2004).…”

Section: Methodsmentioning

confidence: 99%

“…Carboxydothermus hydrogenoformans was the first anaerobic, thermophilic, non-photosynthetic bacterium found that performed this process (Svetlichny et al, 1991). Later, several other organisms performing the reaction of anaerobic CO oxidation with CO 2 and H 2 production were described (Svetlichny et al, 1994;Sokolova et al, 2001Sokolova et al, , 2004.…”

Section: S Rrna Gene Sequence Analysismentioning

confidence: 99%

“…These are representatives of the bacterial genera Carboxydothermus (Svetlichny et al, 1991(Svetlichny et al, , 1994, Caldanaerobacter (formerly Carboxydibrachium pacificum) (Sokolova et al, 2001;Fardeau et al, 2004) and Carboxydocella (Sokolova et al, 2002). Recently, a hyperthermophilic archaeon of the genus Thermococcus capable of growth at the expense of the same reaction was isolated from deepsea hydrothermal vents (Sokolova et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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Thermosinus carboxydivorans gen. nov., sp. nov., a new anaerobic, thermophilic, carbon-monoxide-oxidizing, hydrogenogenic bacterium from a hot pool of Yellowstone National Park

Sokolova

González

Kostrikina

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

107

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A new anaerobic, thermophilic, facultatively carboxydotrophic bacterium, strain Nor1 T , was isolated from a hot spring at Norris Basin, Yellowstone National Park. Cells of strain Nor1 T were curved motile rods with a length of 2?6-3 mm, a width of about 0?5 mm and lateral flagellation. The cell wall structure was of the Gram-negative type. Strain Nor1 T was thermophilic (temperature range for growth was 40-68 6C, with an optimum at 60 6C) and neutrophilic (pH range for growth was 6?5-7?6, with an optimum at 6?8-7?0). It grew chemolithotrophically on CO (generation time, 1?15 h), producing equimolar quantities of H 2 and CO 2 according to the equation CO+H 2 ORCO 2 +H 2 . During growth on CO in the presence of ferric citrate or amorphous ferric iron oxide, strain Nor1 T reduced ferric iron but produced H 2 and CO 2 at a ratio close to 1 : 1, and growth stimulation was slight. Growth on CO in the presence of sodium selenite was accompanied by precipitation of elemental selenium. Elemental sulfur, thiosulfate, sulfate and nitrate did not stimulate growth of strain Nor1 T on CO and none of these chemicals was reduced. Strain Nor1 T was able to grow on glucose, sucrose, lactose, arabinose, maltose, fructose, xylose and pyruvate, but not on cellobiose, galactose, peptone, yeast extract, lactate, acetate, formate, ethanol, methanol or sodium citrate. During glucose fermentation, acetate, H 2 and CO 2 were produced. Thiosulfate was found to enhance the growth rate and cell yield of strain Nor1 T when it was grown on glucose, sucrose or lactose; in this case, acetate, H 2 S and CO 2 were produced. In the presence of thiosulfate or ferric iron, strain Nor1 T was also able to grow on yeast extract. Lactate, acetate, formate and H 2 were not utilized either in the absence or in the presence of ferric iron, thiosulfate, sulfate, sulfite, elemental sulfur or nitrate. Growth was completely inhibited by penicillin, ampicillin, streptomycin, kanamycin and neomycin. The DNA G+C content of the strain was 51?7±1 mol%. Analysis of the 16S rRNA gene sequence revealed that strain Nor1 T belongs to the Bacillus-Clostridium phylum of the Gram-positive bacteria. On the basis of the studied phenotypic and phylogenetic features, we propose that strain Nor1 T be assigned to a new genus, Thermosinus gen. nov. The type species is Thermosinus carboxydivorans sp. nov. (type strain, Nor1 T =DSM 14886 T =VKM B-2281 T ).

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Ni,Fe‐Containing Carbon Monoxide Dehydrogenases

Jeoung

Dobbek

2004

Handbook of Metalloproteins

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Carbon monoxide can be used by various aerobic bacteria, anaerobic bacteria, and archaea as their sole source of carbon and energy. Carbon monoxide dehydrogenases (CODHases) are the central enzymes in the use of carbon monoxide by microorganisms and they catalyze the reversible oxidation of carbon monoxide with water to carbon dioxide, two protons and two electrons. Two principal types of CODHases can be distinguished by their metal cofactors, sequences, specific activity, and stability in the presence of dioxygen. Whereas CODHases containing molybdenum, copper, iron, and flavin are found in aerobic bacteria, anaerobic microorganisms utilize oxygen‐sensitive CODHases containing nickel and iron. The active site of Ni,Fe‐containing CODHases harbors an asymmetric [NiFe 4 S 4/5 ] cluster in which an Ni 2+ ion coordinated by three to four sulfur ligands is an integral constituent. A bimetallic site consisting of the Ni 2+ ion and a histidine and cysteine coordinated Fe 2+ ion (ferrous component II), located on one side of the cluster, is responsible for the binding and activation of carbon monoxide and water. In addition to the active site cluster, three [4Fe–S] clusters are found in the homodimer, of which one [4Fe–4S] cluster covalently links the two monomers.

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Carboxydothermus hydrogenoformans gen. nov., sp. nov., a CO-utilizing Thermophilic Anaerobic Bacterium from Hydrothermal Environments of Kunashir Island

Cited by 161 publications

References 21 publications

Carboxydothermus pertinax sp. nov., a thermophilic, hydrogenogenic, Fe(III)-reducing, sulfur-reducing carboxydotrophic bacterium from an acidic hot spring

Carboxydothermus pertinax sp. nov., a thermophilic, hydrogenogenic, Fe(III)-reducing, sulfur-reducing carboxydotrophic bacterium from an acidic hot spring

Thermosinus carboxydivorans gen. nov., sp. nov., a new anaerobic, thermophilic, carbon-monoxide-oxidizing, hydrogenogenic bacterium from a hot pool of Yellowstone National Park

Ni,Fe‐Containing Carbon Monoxide Dehydrogenases

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