Carboxydothermus siderophilus sp. nov., a thermophilic, hydrogenogenic, carboxydotrophic, dissimilatory Fe(III)-reducing bacterium from a Kamchatka hot spring

Slepova, Tatiana V.; Sokolova, T. G.; Колганова, Т. В.; Tourova, T. P.; Bonch-Osmolovskaya, E. A.

doi:10.1099/ijs.0.000620-0

Cited by 36 publications

(21 citation statements)

References 18 publications

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“…At the time of writing, the genus Carboxydothermus contains three species with validly published names: C. hydrogenoformans (Svetlichny et al, 1991), C. ferrireducens (Slobodkin et al, 2006) and C. siderophilus (Slepova et al, 2009). Strain R1 T 5DSM 7242 T , initially described as 'Carboxydothermus restrictus' (Svetlichny et al, 1994), was later shown to belong to a different phylogenetic lineage and was reclassified as Thermolithobacter carboxydivorans (Sokolova et al, 2007).…”

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confidence: 99%

Carboxydothermus islandicus sp. nov., a thermophilic, hydrogenogenic, carboxydotrophic bacterium isolated from a hot spring

Novikov

Sokolova

Lebedinsky

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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An anaerobic, thermophilic bacterium, strain SET IS-9T, was isolated from an Icelandic hot spring. Cells of strain SET IS-9T are short, slightly curved, motile rods. The strain grows chemolithotrophically on CO, producing equimolar quantities of H2 and CO2. It also grows fermentatively on lactate or pyruvate in the presence of yeast extract (0.2 g l−1). Products of pyruvate fermentation are acetate, CO2 and H2. Growth occurs at 50–70 °C, with an optimum at 65 °C, and at pH 5.0–8.0, with an optimum at pH 5.5–6.0. The generation time during chemolithotrophic growth on CO under optimal conditions is 2.0 h. 16S rRNA gene sequence analysis suggested that the organism belongs to the genus Carboxydothermus. On the basis of phenotypic features and phylogenetic analysis, Carboxydothermus islandicus sp. nov. is proposed, with the type strain SET IS-9T ( = DSM 21830T = VKM B-2561T). An emended description of the genus Carboxydothermus is also given.

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confidence: 99%

Carboxydothermus islandicus sp. nov., a thermophilic, hydrogenogenic, carboxydotrophic bacterium isolated from a hot spring

Novikov

Sokolova

Lebedinsky

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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“…Recent studies have shown physiological diversity in situations where hydrogenogenic growth can also occur simultaneously with the reduction of various electron acceptors (reviewed by Oelgeschläger & Rother, 2008;Sokolova et al, 2009). For example, ferric iron is reduced by Carboxydothermus siderophilus (Slepova et al, 2009) and Thermosinus carboxydivorans (Sokolova et al, 2004b), and elemental sulfur is reduced by some strains of the archaeal genus Thermococcus (Sokolova et al, 2004a;Lee et al, 2008). At present, there are no reports on carboxydotrophic bacteria isolated from acidic hydrothermal environments that are usually rich in ferric iron and elemental sulfur (Stetter, 2005).…”

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confidence: 99%

“…Strains: 1, Ug1 T ; 2, C. hydrogenoformans Z-2901 T ; 3, C. ferrireducens JW/AS-Y7 T ; 4, C. siderophilus 1315 T ; and 5, C. islandicus SET IS-9 T . Data for reference strains are taken from Svetlichny et al (1991), Henstra & Stams (2004), Slobodkin et al (1997Slobodkin et al ( , 2006, Slepova et al (2009) …”

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confidence: 99%

Carboxydothermus pertinax sp. nov., a thermophilic, hydrogenogenic, Fe(III)-reducing, sulfur-reducing carboxydotrophic bacterium from an acidic hot spring

Yoneda

Yoshida

Kawaichi

et al. 2012

International Journal of Systematic and Evolutionary Microbiology

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A novel anaerobic, Fe(III)-reducing, hydrogenogenic, carboxydotrophic bacterium, designated strain Ug1 T , was isolated from a volcanic acidic hot spring in southern Kyushu Island, Japan. Cells of the isolate were rod-shaped (1.0-3.0 mm long) and motile due to peritrichous flagella. Strain Ug1 T grew chemolithoautotrophically on CO (100 % in the gas phase) with reduction of ferric citrate, amorphous iron (III) oxide, 9,10-anthraquinone 2,6-disulfonate, thiosulfate or elemental sulfur. No carboxydotrophic growth occurred with sulfate, sulfite, nitrate or fumarate as electron acceptor. During growth on CO, H 2 and CO 2 were produced. Growth occurred on molecular hydrogen as an energy source and carbon dioxide as a sole carbon source. Growth was observed on various organic compounds under an N 2 atmosphere with the reduction of ferric iron. The temperature range for carboxydotrophic growth was 50-70 6C, with an optimum at 65 6C. The pH 25 6C range for growth was 4.6-8.6, with an optimum between 6.0 and 6.5. The doubling time under optimum conditions using CO with ferric citrate was 1.5 h. The DNA G+C content was 42.2 mol%. Analysis of 16S rRNA gene sequences demonstrated that this strain belongs to the thermophilic carboxydotrophic bacterial genus Carboxydothermus, with sequence similarities of 94.1-96.6 % to members of this genus. The isolate can be distinguished from other members of the genus Carboxydothermus by its ability to grow with elemental sulfur or thiosulfate coupled to CO oxidation. On the basis of phylogenetic analysis and unique physiological features, the isolate represents a novel species of the genus Carboxydothermus for which the name Carboxydothermus pertinax sp. nov. is proposed; the type strain of the novel species is Ug1 T (5DSM 23698 T 5NBRC 107576 T ).

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“…Essential genes and reactions in the EFM pathways could not be deleted [43]. For the model of acetyl-CoA pathway, elementary mode (EM) has been systematically used to examine gene deletion that influence the hydrogen yield of C. hydrogenoformans as well as to identify a set of multiple gene knockout which results in higher product yield.…”

Section: Resultsmentioning

confidence: 99%

Elementary Flux Mode Analysis of Acetyl-CoA Pathway in Carboxydothermus hydrogenoformans Z-2901

Perumal

Selvaraj

Kumar

2014

Advances in Bioinformatics

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Carboxydothermus hydrogenoformans is a carboxydotrophic hydrogenogenic bacterium species that produces hydrogen molecule by utilizing carbon monoxide (CO) or pyruvate as a carbon source. To investigate the underlying biochemical mechanism of hydrogen production, an elementary mode analysis of acetyl-CoA pathway was performed to determine the intermediate fluxes by combining linear programming (LP) method available in CellNetAnalyzer software. We hypothesized that addition of enzymes necessary for carbon monoxide fixation and pyruvate dissimilation would enhance the theoretical yield of hydrogen. An in silico gene knockout of pyk, pykC, and mdh genes of modeled acetyl-CoA pathway allows the maximum theoretical hydrogen yield of 47.62 mmol/gCDW/h for 1 mole of carbon monoxide (CO) uptake. The obtained hydrogen yield is comparatively two times greater than the previous experimental data. Therefore, it could be concluded that this elementary flux mode analysis is a crucial way to achieve efficient hydrogen production through acetyl-CoA pathway and act as a model for strain improvement.

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Carboxydothermus siderophilus sp. nov., a thermophilic, hydrogenogenic, carboxydotrophic, dissimilatory Fe(III)-reducing bacterium from a Kamchatka hot spring

Abstract: Carboxydothermus siderophilus sp. nov., a thermophilic, hydrogenogenic, carboxydotrophic, dissimilatory Fe(III)-reducing bacterium from a Kamchatka hot spring

Cited by 36 publications

References 18 publications

Carboxydothermus islandicus sp. nov., a thermophilic, hydrogenogenic, carboxydotrophic bacterium isolated from a hot spring

Carboxydothermus islandicus sp. nov., a thermophilic, hydrogenogenic, carboxydotrophic bacterium isolated from a hot spring

Carboxydothermus pertinax sp. nov., a thermophilic, hydrogenogenic, Fe(III)-reducing, sulfur-reducing carboxydotrophic bacterium from an acidic hot spring

Elementary Flux Mode Analysis of Acetyl-CoA Pathway in Carboxydothermus hydrogenoformans Z-2901

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