Carboxydovores from the Pseudomonadota colonise volcanic soils during succession

Abstract

Carbon monoxide (CO) degrading microorganisms are present in volcanic deposits throughout succession, with vegetation and soil influencing the communities present. The carboxydovores are a subset of CO degraders that use CO only as an energy source, raising the question of how the physiological and metabolic features of the carboxydovores can make these bacteria more competitive in harsh volcanic ecosystems. An enrichment strategy was modified, which enabled the isolation of two carboxydovore representatives from genera that were abundant in the native soils,Cupriavidussp. CV2T(92.3% ANI vs.Cupriavidus basilensisDSM 11853) and a putative strain ofParaburkholderia terrae(Pb. terraeCOX) (96.42% ANI vs.Pb. terraeKU-64T). These isolates oxidise CO across a very broad range of concentrations, and genome sequence analysis indicated that they use form-I carbon monoxide dehydrogenase (CODH) to do so.Cupriavidussp. CV2TandPb. terraeCOX each oxidised CO specifically at stationary phase, but the conditions for induction of CODH expression were distinct.Cupriavidussp. CV2Texpressed CODH only in the presence of CO, whilePb. terraeCOX expressed CODH regardless of the presence of CO. Based on metabolic and phylogenetic analyses,Cupriavidussp. CV2Tis recommended as a novel species within the genusCupriavidus. Therefore, we propose the nameCupriavidus ulmosensissp. nov. for the type strain CV2T(= NCIMB 15506T, = CECT 30956T). This study provides valuable insights into the physiology and metabolism of carboxydovores, which colonise volcanic ecosystems during succession.ImportanceVolcanic ecosystems harbour many bacteria that contribute to the environmentally important process of carbon monoxide (CO) oxidation. We demonstrate a modified method for isolating bacteria, which consume CO at very low concentrations as a supplementary energy source (carboxydovory), leading to the isolation of two novel strains (Cupriavidussp. CV2TandParaburkholderia terraeCOX) from volcanic strata that formed in 1917 and 2015, respectively. The conditions under which CO consumption occurs were investigated; each strain consumed CO during stationary phase, butPb. terraeCOX consumed CO regardless of the prior growth conditions whileCupriavidussp. CV2 was more controlled.Cupriavidussp. CV2 is a type strain of a new species,Cupriavidus ulmosensisstr. CV2, which demonstrates relatively high tolerance for CO. These strains provide the basis for further study of the physiology, metabolism, and genetics of CO oxidation by carboxydovores, and will help us to understand how bacteria colonise harsh volcanic ecosystems.