Carboxyethyl γ - Aminobutyric Acid, a polyamine derivative, improves the recovery of EBV-transformed lymphocytes

IL-6 was demonstrated to promote growth of EBV transformed lymphocytes. However IL-6 was ineffective at promoting growth of EBV transformed lymphocytes cultured at the single cell level under limiting dilution conditions. On the contrary, HECS, which is known to contain IL-6, supported very efficiently the growth of 1-2 EBV transformed cells. When IL-6 was removed from HECS, by using specific antibodies, no reduction in HECS activity was observed, indicating that probably more than one growth factor is required to support the growth of EBV transformed cells cultured at very low cell numbers in the absence of feeder cells.

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A growth factor different from IL-6 promotes the growth of Epstein-Barr virus-transformed cells at low cell density

Negri

Cerino

et al. 1991

Cytotechnology

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Interleukin-6 (IL-6) was recently described to support the growth of Epstein-Barr (EBV) transformed lymphocytes. However no effect was reported on cloning of EBV transformed lymphocytes under limiting dilution conditions. In this paper we demonstrate that the Supernatant of Human Endothelial Cells (HECS) contains a factor(s) different from IL-6 which induces high cloning efficiency of EBV transformed cell lines, cultured under limiting dilution conditions. Furthermore HECS is superior to IL-6 in all the passages of EBV lymphocytes transformation.

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Carboxyethyl γ - Aminobutyric Acid, a polyamine derivative, improves the recovery of EBV-transformed lymphocytes

Cited by 5 publications

References 8 publications

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Factor(s) required by EBV transformed lymphocytes to grow under limiting dilution conditions

A growth factor different from IL-6 promotes the growth of Epstein-Barr virus-transformed cells at low cell density

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