Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity

Manakhov, Anton; Kedroňová, Eva; Medalová, Jiřina; Černochová, Petra; Obrusník, Adam; Michlíček, Miroslav; Shtansky, Dmitry V.; Zajı́čková, Lenka

doi:10.1016/j.matdes.2017.06.057

Cited by 49 publications

(56 citation statements)

References 36 publications

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“…PCL polymer (Sigma Aldrich, Germany) with a molecular weight of approximately 80,000 g/mol in amount of 9 wt.% was dissolved in a 2:1 mixture of acetic acid (99%, Sigma Aldrich, Germany) and formic acid (98%, Sigma Aldrich, Germany). The electrospinning process was carried out on a Nanospider™ NSLAB 500 (ELMARCO, Czech Republic) machine using a 20 cm-long wired electrode at a voltage of 55 kV according to the protocol available elsewhere [21]. The distance between high voltage and ground electrodes was set at 100 mm.…”

Section: Electrospinning Of Poly-ε-caprolactone (Pcl) Nanofibersmentioning

confidence: 99%

“…Previous studies suggested that the wettability of a polymer surface can be improved by the deposition of hydrophilic film [19]. Unlike high-energy ion irradiation of polymers, which can lead to its destruction and toxicity [20], magnetron Nanomaterials 2019, 9,1769 3 of 16 sputtering with carefully optimized processing parameters is an effective method to coat biodegradable polymer with highly adhesive biocompatible film [21]. Another promising approach for the PCL surface modification is COOH plasma polymerization.…”

Section: Introductionmentioning

confidence: 99%

“…In the present study, two approaches to endow PCL with enhance bioactivity were used and compared: (i) atmospheric pressure plasma copolymerization of CO 2 and C 2 H 4 to form COOH-containing polymer and (ii) magnetron sputtering of TiC-CaO-Ti 3 PO x target to deposit TiCaPCON film. [21]. Another promising approach for the PCL surface modification is COOH plasma polymerization.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds

Permyakova

Kiryukhantsev-Korneev

Gudz

et al. 2019

Nanomaterials

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Due to their good mechanical stability compared to gelatin, collagen or polyethylene glycol nanofibers and slow degradation rate, biodegradable poly-ε-caprolactone (PCL) nanofibers are promising material as scaffolds for bone and soft-tissue engineering. Here, PCL nanofibers were prepared by the electrospinning method and then subjected to surface functionalization aimed at improving their biocompatibility and bioactivity. For surface modification, two approaches were used: (i) COOH-containing polymer was deposited on the PCL surface using atmospheric pressure plasma copolymerization of CO 2 and C 2 H 4 , and (ii) PCL nanofibers were coated with multifunctional bioactive nanostructured TiCaPCON film by magnetron sputtering of TiC-CaO-Ti 3 PO x target. To evaluate bone regeneration ability in vitro, the surface-modified PCL nanofibers were immersed in simulated body fluid (SBF, 1×) for 21 days. The results obtained indicate different osteoblastic and epithelial cell response depending on the modification method. The TiCaPCON-coated PCL nanofibers exhibited enhanced adhesion and proliferation of MC3T3-E1 cells, promoted the formation of Ca-based mineralized layer in SBF and, therefore, can be considered as promising material for bone tissue regeneration. The PCL-COOH nanofibers demonstrated improved adhesion and proliferation of IAR-2 cells, which shows their high potential for skin reparation and wound dressing.

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Section: Electrospinning Of Poly-ε-caprolactone (Pcl) Nanofibersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds

Permyakova

Kiryukhantsev-Korneev

Gudz

et al. 2019

Nanomaterials

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“…A proper choice of the plasma source allows various kinds of functional groups to be attached on the material exterior, resulting in high biocompatibility or the possibility of immobilizing bioactives such as ECM proteins, cationized gelatin [74], and RGD peptides [75]. Carboxyl groups or amine groups can be assembled by plasma treatments with ammonia, oxygen, or air, and they can improve cell proliferation and adhesion [68]. For example, PLGA nanofiber was treated with oxygen or ammonia plasma on its surface to improve its surface hydrophilicity, leading to cell adhesion and proliferation rate of fibroblast cells [8].…”

Section: Surface Modification: Plasma and Laser Treatmentmentioning

confidence: 99%

“…Recently, plasma polymer deposition has been used to increase the density of functional groups on surfaces. Manakhov et al demonstrated the reproducible deposition of amine and carboxyl plasma coating on PCL nanofibers via plasma polymer deposition, resulting in a high density of functional groups on the surface [68]. Besides plasma treatment, laser treatment is also a potential technique for enhancing fiber characteristics, especially porosity.…”

Section: Surface Modification: Plasma and Laser Treatmentmentioning

confidence: 99%

Recent Developments in Nanofiber Fabrication and Modification for Bone Tissue Engineering

Udomluck

Koh

Lim

et al. 2019

IJMS

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Bone tissue engineering is an alternative therapeutic intervention to repair or regenerate lost bone. This technique requires three essential components: stem cells that can differentiate into bone cells, growth factors that stimulate cell behavior for bone formation, and scaffolds that mimic the extracellular matrix. Among the various kinds of scaffolds, highly porous nanofibrous scaffolds are a potential candidate for supporting cell functions, such as adhesion, delivering growth factors, and forming new tissue. Various fabricating techniques for nanofibrous scaffolds have been investigated, including electrospinning, multi-axial electrospinning, and melt writing electrospinning. Although electrospun fiber fabrication has been possible for a decade, these fibers have gained attention in tissue regeneration owing to the possibility of further modifications of their chemical, biological, and mechanical properties. Recent reports suggest that post-modification after spinning make it possible to modify a nanofiber's chemical and physical characteristics for regenerating specific target tissues. The objectives of this review are to describe the details of recently developed fabrication and post-modification techniques and discuss the advanced applications and impact of the integrated system of nanofiber-based scaffolds in the field of bone tissue engineering. This review highlights the importance of nanofibrous scaffolds for bone tissue engineering.

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The role of plasma‐induced surface chemistry on polycaprolactone nanofibers to direct chondrogenic differentiation of human mesenchymal stem cells

Asadian,

Tomasina,

Onyshchenko

et al. 2023

J Biomedical Materials Res

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Bone marrow‐derived mesenchymal stromal cells (BMSCs) are extensively being utilized for cartilage regeneration owing to their excellent differentiation potential and availability. However, controlled differentiation of BMSCs towards cartilaginous phenotypes to heal full‐thickness cartilage defects remains challenging. This study investigates how different surface properties induced by either coating deposition or biomolecules immobilization onto nanofibers (NFs) could affect BMSCs chondro‐inductive behavior. Accordingly, electrospun poly(ε‐caprolactone) (PCL) NFs were exposed to two surface modification strategies based on medium‐pressure plasma technology. The first strategy is plasma polymerization, in which cyclopropylamine (CPA) or acrylic acid (AcAc) monomers were plasma polymerized to obtain amine‐ or carboxylic acid‐rich NFs, respectively. The second strategy uses a combination of CPA plasma polymerization and a post‐chemical technique to immobilize chondroitin sulfate (CS) onto the NFs. These modifications could affect surface roughness, hydrophilicity, and chemical composition while preserving the NFs' nano‐morphology. The results of long‐term BMSCs culture in both basic and chondrogenic media proved that the surface modifications modulated BMSCs chondrogenic differentiation. Indeed, the incorporation of polar groups by different modification strategies had a positive impact on the cell proliferation rate, production of the glycosaminoglycan matrix, and expression of extracellular matrix proteins (collagen I and collagen II). The chondro‐inductive behavior of the samples was highly dependent on the nature of the introduced polar functional groups. Among all samples, carboxylic acid‐rich NFs promoted chondrogenesis by higher expression of aggrecan, Sox9, and collagen II with downregulation of hypertrophic markers. Hence, this approach showed an intrinsic potential to have a non‐hypertrophic chondrogenic cell phenotype.

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Carboxyl-anhydride and amine plasma coating of PCL nanofibers to improve their bioactivity

Cited by 49 publications

References 36 publications

Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds

Comparison of Different Approaches to Surface Functionalization of Biodegradable Polycaprolactone Scaffolds

Recent Developments in Nanofiber Fabrication and Modification for Bone Tissue Engineering

The role of plasma‐induced surface chemistry on polycaprolactone nanofibers to direct chondrogenic differentiation of human mesenchymal stem cells

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