Carboxylases in Natural and Synthetic Microbial Pathways

Erb, Tobias J.

doi:10.1128/aem.05702-11

Cited by 182 publications

(175 citation statements)

References 126 publications

(183 reference statements)

Supporting

Mentioning

173

Contrasting

Unclassified

Order By: Relevance

“…In recent years, carboxylating enzymes and autotrophic CO 2 -fixation pathways have attracted much attention because of their potential future applications for the production of industrial chemicals using synthetic biology approaches (39)(40)(41). Autotrophy based on acetyl-CoA/propionyl-CoA and S10-S12.…”

Section: Discussionmentioning

confidence: 99%

Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO ₂ fixation

Könneke

Schubert

Brown

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

419

342

View full text Add to dashboard Cite

Archaea of the phylum Thaumarchaeota are among the most abundant prokaryotes on Earth and are widely distributed in marine, terrestrial, and geothermal environments. All studied Thaumarchaeota couple the oxidation of ammonia at extremely low concentrations with carbon fixation. As the predominant nitrifiers in the ocean and in various soils, ammonia-oxidizing archaea contribute significantly to the global nitrogen and carbon cycles. Here we provide biochemical evidence that thaumarchaeal ammonia oxidizers assimilate inorganic carbon via a modified version of the autotrophic hydroxypropionate/hydroxybutyrate cycle of Crenarchaeota that is far more energy efficient than any other aerobic autotrophic pathway. The identified genes of this cycle were found in the genomes of all sequenced representatives of the phylum Thaumarchaeota, indicating the environmental significance of this efficient CO 2 -fixation pathway. Comparative phylogenetic analysis of proteins of this pathway suggests that the hydroxypropionate/hydroxybutyrate cycle emerged independently in Crenarchaeota and Thaumarchaeota, thus supporting the hypothesis of an early evolutionary separation of both archaeal phyla. We conclude that high efficiency of anabolism exemplified by this autotrophic cycle perfectly suits the lifestyle of ammonia-oxidizing archaea, which thrive at a constantly low energy supply, thus offering a biochemical explanation for their ecological success in nutrient-limited environments.Nitrosopumilus maritimus | autotrophy

show abstract

Section: Discussionmentioning

confidence: 99%

Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO ₂ fixation

Könneke

Schubert

Brown

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

419

342

View full text Add to dashboard Cite

show abstract

“…They impact substantially to the global carbon cycle, in which enzymes play crucial roles in catalyzing the CO2 fixation [12]. Beside, a large number of enzymes, such as decarboxylase enzymes in carboxylation reactions derived from catabolic pathways, utilize CO2 or HCO3 − as a reaction substrate and do not belong to chemoautotrophic pathways [2,12,13].…”

Section: Natural Conversion Of Co2 In Cellsmentioning

confidence: 99%

Recent Progress and Novel Applications in Enzymatic Conversion of Carbon Dioxide

et al. 2017

View full text Add to dashboard Cite

Turning carbon dioxide (CO 2 ) into fuels and chemicals using chemical, photochemical, electrochemical, and enzymatic methods could be used to recycle large quantities of carbon. The enzymatic method, which is inspired by cellular CO 2 metabolism, has attracted considerable attention for efficient CO 2 conversion due to improved selectivity and yields under mild reaction conditions. In this review, the research progress of green and potent enzymatic conversion of CO 2 into useful fuels and chemicals was discussed. Furthermore, applications of the enzymatic conversion of CO 2 to assist in CO 2 capture and sequestration were highlighted. A summary including the industrial applications, barriers, and some perspectives on the research and development of the enzymatic approach to convert CO 2 were introduced.

show abstract

“…In addition to pyruvate carboxylase and PEP carboxykinase, genome analysis of MED193 showed the presence of genes involved in the ethylmalonyl-CoA pathway, which allows bacteria lacking the glyoxylate shunt to grow on carbon compounds like acetate (22,31,66,67). An important step in this pathway is the fixation of 1 molecule of CO 2 and 1 molecule of bicarbonate per 3 molecules of acetate, whereby it fulfills an important anaplerotic reaction (24).…”

Section: Maritimibacter Alkaliphilus Htcc2654mentioning

confidence: 99%

“…Although rather unexplored in the field of marine microbial ecology, anaplerotic reactions, including anaplerotic CO 2 fixation, are a conserved feature in the metabolism of all living organisms (21,22). Thus, most heterotrophic bacteria assimilate CO 2 into biomass through various carboxylase reactions (23)(24)(25).…”

mentioning

confidence: 99%

Dynamics of Metabolic Activities and Gene Expression in the Roseobacter Clade Bacterium Phaeobacter sp. Strain MED193 during Growth with Thiosulfate

Muthusamy

Baltar

González

et al. 2014

Appl Environ Microbiol

View full text Add to dashboard Cite

bMetagenomic analyses of surface seawater reveal that genes for sulfur oxidation are widespread in bacterioplankton communities. However, little is known about the metabolic processes used to exploit the energy potentially gained from inorganic sulfur oxidation in oxic seawater. We therefore studied the sox gene system containing Roseobacter clade isolate Phaeobacter sp. strain MED193 in acetate minimal medium with and without thiosulfate. The addition of thiosulfate enhanced the bacterial growth yields up to 40% in this strain. Concomitantly, soxB and soxY gene expression increased about 8-fold with thiosulfate and remained 11-fold higher than that in controls through stationary phase. At stationary phase, thiosulfate stimulated protein synthesis and anaplerotic CO 2 fixation rates up to 5-and 35-fold, respectively. Several genes involved in anaplerotic CO 2 fixation (i.e., pyruvate carboxylase, propionyl coenzyme A [CoA], and crotonyl-CoA carboxylase) were highly expressed during active growth, coinciding with high CO 2 fixation rates. The high expression of key genes in the ethylmalonyl-CoA pathway suggests that this is an important pathway for the utilization of two-carbon compounds in Phaeobacter sp. MED193. Overall, our findings imply that Roseobacter clade bacteria carrying sox genes can use their lithotrophic potential to gain additional energy from sulfur oxidation for both increasing their growth capacity and improving their long-term survival.

show abstract

Carboxylases in Natural and Synthetic Microbial Pathways

Cited by 182 publications

References 126 publications

Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO ₂ fixation

Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO ₂ fixation

Recent Progress and Novel Applications in Enzymatic Conversion of Carbon Dioxide

Dynamics of Metabolic Activities and Gene Expression in the Roseobacter Clade Bacterium Phaeobacter sp. Strain MED193 during Growth with Thiosulfate

Contact Info

Product

Resources

About

Carboxylases in Natural and Synthetic Microbial Pathways

Cited by 182 publications

References 126 publications

Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO 2 fixation

Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO 2 fixation

Recent Progress and Novel Applications in Enzymatic Conversion of Carbon Dioxide

Dynamics of Metabolic Activities and Gene Expression in the Roseobacter Clade Bacterium Phaeobacter sp. Strain MED193 during Growth with Thiosulfate

Contact Info

Product

Resources

About

Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO ₂ fixation

Ammonia-oxidizing archaea use the most energy-efficient aerobic pathway for CO ₂ fixation