Carboxylate‐Functionalized Iron Oxide Nanoparticles in Surface‐Assisted Laser Desorption/Ionization Mass Spectrometry for the Analysis of Small Biomolecules

Chiu, Yu-Chih; Chen, Yu-Chie

doi:10.1080/00032710701792653

Cited by 15 publications

(5 citation statements)

References 27 publications

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“…Presence of a proton donor and high extinction coefficients at laser exciting wavelengths are crucial for MALDI [34] and SALDI-MS [22,35]. CHCA is widely used because of its high UV-absorbing property and its ability to function as an effective proton donor.…”

Section: Functionalization Of Nanoparticles and Direct Ionization Of mentioning

confidence: 99%

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CHCA-modified Au nanoparticles for laser desorption ionization mass spectrometric analysis of peptides

Duan

Linman

Chen

et al. 2009

J. Am. Soc. Mass Spectrom.

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Gold nanoparticles (AuNPs) have been studied as a potential solid-state matrix for laser desorption/ionization mass spectrometry (LDI-MS) but the efficiency in ionization remains low. In this report, AuNPs are capped by a self-assembled monolayer of cysteamine and modified with ␣-cyano-4-hydroxycinnanic acid (CHCA) for effective MALDI measurements. CHCA-terminated AuNPs offer marked improvement on peptide ionization compared with citrate-capped or cysteamine-capped AuNPs. The coating also effectively suppresses formation of Au cluster ions and analyte fragment ions, leading to cleaner mass spectra. Addition of glycerol and citric acid to the peptide/AuNPs sample further improves the performance of these AuNPs for LDI-MS analysis. Glycerol appears to enhance the dispersion of AuNPs in sample spots, increasing the sample ionization and shot-to-shot reproducibility, while citric acid serves as an external proton donor, providing high production of protonated analyte ions and reducing fragmentation of peptides on the nanoparticle-based surface. Optimal ratios of citric acid, glycerol, and AuNPs in sample solution have been systematically studied. A more than 10-fold increase for desorption ionization of peptides can be achieved by combining 5% glycerol and 20 mM citric acid with the CHCA-terminated AuNPs. The applicability of the CHCA-AuNPs for LDI-MS analysis of protein digests has also been demonstrated. This work shows the potential of AuNPs for SALDI-MS analysis, and the improvement with chemical functionalization, controlled dispersion, and use of an effective proton donor. (J Am Soc Mass

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Section: Functionalization Of Nanoparticles and Direct Ionization Of mentioning

confidence: 99%

“…Additional tests were carried out using citric acid as an effective proton source for SALDI-matrixes [35,44,45]. Figure 4b shows the improvement of signal-tonoise ratio for protonated peptide ions when citric acid was added.…”

Section: Improving Dispersion Of Aunps On the Sample Platementioning

confidence: 99%

CHCA-modified Au nanoparticles for laser desorption ionization mass spectrometric analysis of peptides

Duan

Linman

Chen

et al. 2009

J. Am. Soc. Mass Spectrom.

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“…Owing to the requirement of using these inorganic nanomaterials in SALDI-MS, affinity-based SALDI-MS based on the use of nanomaterials as the SALDI-assisting materials and nanoprobes for trapping analytes of interest has been reported. [13][14][15][16][17][18][19][20]22,23 Therefore, trace analytes can be concentrated by the SALDI-assisting materials followed by SALDI-MS analysis, whereas the interference contributed by nontarget species can be reduced. However, matrices used in conventional MALDI-MS do not possess such dual functions.…”

Section: ■ Introductionmentioning

confidence: 99%

“…SALDI-assisting materials serve as energy absorbers during analysis to assist analyte desorption. Thus, various inorganic materials, such as metal nanoparticles, , metal oxide nanomaterials, , metal–organic frameworks, , and carbon-based materials, − have been demonstrated to be effective assisting materials in SALDI-MS analysis. Owing to the requirement of using these inorganic nanomaterials in SALDI-MS, affinity-based SALDI-MS based on the use of nanomaterials as the SALDI-assisting materials and nanoprobes for trapping analytes of interest has been reported. − ,, Therefore, trace analytes can be concentrated by the SALDI-assisting materials followed by SALDI-MS analysis, whereas the interference contributed by nontarget species can be reduced.…”

Section: Introductionmentioning

confidence: 99%

“…Thus, various inorganic materials, such as metal nanoparticles, , metal oxide nanomaterials, , metal–organic frameworks, , and carbon-based materials, − have been demonstrated to be effective assisting materials in SALDI-MS analysis. Owing to the requirement of using these inorganic nanomaterials in SALDI-MS, affinity-based SALDI-MS based on the use of nanomaterials as the SALDI-assisting materials and nanoprobes for trapping analytes of interest has been reported. − ,, Therefore, trace analytes can be concentrated by the SALDI-assisting materials followed by SALDI-MS analysis, whereas the interference contributed by nontarget species can be reduced. However, matrices used in conventional MALDI-MS do not possess such dual functions.…”

Section: Introductionmentioning

confidence: 99%

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Magnetic Graphene Oxide-Based Affinity Surface-Assisted Laser Desorption/Ionization Mass Spectrometry for Screening of Aflatoxin B1 from Complex Samples

2021

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Aflatoxin B1 (AFB1), commonly found in agriculture products, has been considered as a carcinogen. Thus, to develop analytical methods that can be used to rapidly screen the presence of AFB1 in complex samples is important. Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) uses inorganic materials as assisting materials to facilitate desorption/ionization of analytes. The feasibility of using GO as the affinity probe against AFB1 and as the assisting material in SALDI-MS analysis was first demonstrated. We also explored a facile method to impose magnetism on GO to generate magnetic GO (MGO) nanoprobes by simply incubating GO in aqueous FeCl3 under microwave heating. The generated MGO nanoprobes possessed magnetism and were capable of enriching trace AFB1 from complex samples. AFB1 enrichment took only 6 min by incubating MGO with samples under microwave heating (power = 90 W). Followed by magnetic isolation, the isolated conjugates were ready for SALDI-MS analysis. The enrichment steps including trapping and isolation can be completed within ∼10 min. The lowest detectable concentration of our method toward AFB1 was ∼1 nM. Results also showed that AFB1 can be selectively detected from complex samples, including cell lysates of fungal spores, AFB1-spiked peanut, and wheat samples, by using the developed method. The selectivity of our method against AFB1 from the samples containing other toxins including aflatoxin G1 and ochratoxin A was also examined. According to these results, we believe that the developed method should have the potential to be used for rapid screening of AFB1 from real-world samples.

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Ion Sources for Time‐resolved Mass Spectrometry

Urban

Chen

Wang

2016

Time‐Resolved Mass Spectrometry

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Carboxylate‐Functionalized Iron Oxide Nanoparticles in Surface‐Assisted Laser Desorption/Ionization Mass Spectrometry for the Analysis of Small Biomolecules

Cited by 15 publications

References 27 publications

CHCA-modified Au nanoparticles for laser desorption ionization mass spectrometric analysis of peptides

CHCA-modified Au nanoparticles for laser desorption ionization mass spectrometric analysis of peptides

Magnetic Graphene Oxide-Based Affinity Surface-Assisted Laser Desorption/Ionization Mass Spectrometry for Screening of Aflatoxin B1 from Complex Samples

Ion Sources for Time‐resolved Mass Spectrometry

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