Carboxylic acids of different nature induces aggregation of hemoglobin

Hasan, Samra; Fatma, Seerat; Zaman, Masihuz; Khan, Rizwan Hasan; Naeem, Aabgeena

doi:10.1016/j.ijbiomac.2018.07.003

Cited by 10 publications

(5 citation statements)

References 56 publications

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“…At 50 mM of both acids, comprehensive loss of all helical structure and altering to majorly β-sheets was observed which indicated intermolecular β-sheets formation because of aggregates formed. 74 Thus, the decreased absorbance accompanied by a shift in λ max observed as the concentration of mPEG- b -PJL-COOH increased may not indicate an actual decrease in haemolysis rate. However, the structural disruption of the haemoglobin released from lysed RBCs induced by the COOH-pendant renders the UV absorption spectrum of haemoglobin an unreliable methodology for measuring its concentration.…”

Section: Resultsmentioning

confidence: 96%

Functional block copolymer micelles based on poly (jasmine lactone) for improving the loading efficiency of weakly basic drugs

et al. 2022

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Section: Resultsmentioning

confidence: 96%

Functional block copolymer micelles based on poly (jasmine lactone) for improving the loading efficiency of weakly basic drugs

et al. 2022

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“…Protein was excited at 350 nm and spectra were recorded in the emission range 300 to 400 nm using a quartz cell of 1 cm path length. 31 Both the excitation and emission slit widths were set at 5 nm.…”

Section: Fluorescence Spectroscopymentioning

confidence: 99%

The modulation of structural stability of horseradish peroxidase as a consequence of macromolecular crowding

Hasan

Naeem

2021

J of Molecular Recognition

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Macromolecular crowding plays an inevitable role in all biological processes influencing association, conformation, and other characteristics of proteins. Present study is based on the effect of macromolecular crowding on structure of horseradish peroxidase (HRP) enzyme. Concentration-dependent conformational changes induced by crowding agents, dextran 70 and polyethylene glycol (PEG)-4000, were monitored employing a range of biophysical techniques. The intrinsic fluorescence spectra showed transition of protein from native to unfolded state. Marked increase in 8-Anilino-1-naphthalene-sulphonoic acid and Thioflavin T fluorescence indicated presence of non-native moieties with 80 mg/mL dextran. Enhanced absorbance in turbidity, Soret, and Congo red in corroboration with scattering intensity at 350nm results revealed incidence of HRP aggregates. A new peak around 218 nm in CD spectra pointed towards change in secondary structure towards β-sheets. Significant loss of enzyme activity upon structural disruption was seen. Comet assay demonstrated DNA damage and genotoxic nature of HRP aggregates, supporting spectroscopic, and fluorescence results. The normalized resultswere obtained with 120 mg/mL PEG-4000 close to that of native HRP implying no disruptive effect on structure. It can be hypothesized that macromolecular crowding is a vital element, which can have diverse effects. In this study, dextran 70 was observed to have pro-aggregatory effect while enhanced stability of native enzyme was witnessed with PEG. Hence, it can be stated that PEG has potentially better crowder as it helps retain the native enzyme structure. Routine addition of crowding agents is recommended if biological molecules are to be studied under more physiologically appropriate environments.

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“…Carbohydrates, polyols, amino acids, buffers, and surfactants are the major excipients used to stabilize proteins in biopharmaceutical formulations [ 4 ]. These excipients significantly alter the conformational and colloidal stability of proteins and reduce their chemical degradation through interactions with drugs [ [5] , [6] , [7] ]. For example, carbohydrates and polyols stabilize proteins through various alternative mechanisms to improve their colloidal stability [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of polyol excipient stability during storage and use on the quality of biopharmaceutical formulations

Sun

Liao

Jing

et al. 2022

Journal of Pharmaceutical Analysis

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Carboxylic acids of different nature induces aggregation of hemoglobin

Cited by 10 publications

References 56 publications

Functional block copolymer micelles based on poly (jasmine lactone) for improving the loading efficiency of weakly basic drugs

Functional block copolymer micelles based on poly (jasmine lactone) for improving the loading efficiency of weakly basic drugs

The modulation of structural stability of horseradish peroxidase as a consequence of macromolecular crowding

Effects of polyol excipient stability during storage and use on the quality of biopharmaceutical formulations

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