Carboxymethyl Cellulase (CMCase) from UV-irradiation Mutated Bacillus cereus FOA-2 cultivated on Plantain (Musa parasidiaca) Stalk-based Medium: Production, Purification and Characterization

Olukunle, Oluwatoyin Folake; Ayodeji, Adeyemi Oluwadare; Akinloye, Peter O.

doi:10.1016/j.sciaf.2020.e00691

Cited by 10 publications

(5 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hydrolysis indices have been confirmed when evaluating the metabolic activity of B. amyloliquefaciens after using starch hydrolysis ratios [ 40 ]. Additionally, CI has been used in B. cereus [ 41 ] and thermophilic bacteria [ 27 ]. AI was also used in B. cereus [ 42 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Bacteria of Genus Bacillus from Composting Urban Solid Waste and Palm Forest in Northern Peru

Vásquez

Millones

2023

Microorganisms

View full text Add to dashboard Cite

A technical challenge for composting in Peruvian cities with annual temperatures below 20 °C is that the degradation of municipal solid waste (MSW) is slow, so the identification of cold-adapted bacteria would be interesting for use as inoculants in places with these climatic conditions. This study isolated, identified, and evaluated bacterial strains with cellulolytic and amylolytic activities at low temperatures. Bacterial strains were isolated from the Chachapoyas Municipal Composting Plant and soil from the Ocol Palm Forest in northern Peru. The screening was carried out to evaluate the extracellular enzyme activity of the strains at low temperatures, grouping those with cellulolytic and cellulolytic/amylolytic activities. The DNA-barcoding using 16S rRNA and enzyme activity allowed the identification and selection of five species with enzymatic activity at 15 and 20 °C of the genus Bacillus, three with cellulolytic/amylolytic activity (B. wiedmanii, B. subtilis, and B. velezensis), and two with cellulolytic activity (B. safensis subsp. safensis, and B. subtilis). These strains showed tolerance to temperatures below optimum and could be used in further studies as inoculants for composting organic wastes at temperatures below 20 °C.

show abstract

Section: Discussionmentioning

confidence: 99%

Isolation and Identification of Bacteria of Genus Bacillus from Composting Urban Solid Waste and Palm Forest in Northern Peru

Vásquez

Millones

2023

Microorganisms

View full text Add to dashboard Cite

show abstract

“…CMCase describes the activity of the endoglucanase enzyme. (Kirn et al 2018;Olukunle et al 2021), avicel explained the activity of exoglucanase (Sirohi et al 2013), and FPase described total cellulase enzyme activity (Eida et al 2016). FPase was the total activity of cellulase enzymes, which consists of endo-1,4-β-D-glucanase, 1,4-β-D-glucancellobiohydrolase, and β-glucosidase (Reyes et al 2019).…”

Section: Cellulase Enzyme Activity In Anaerobic Fungi Isolated From B...mentioning

confidence: 99%

“…Cellulase is an important enzyme for livestock-fed high-fiber feeds. Olukunle et al (2021) stated that it is very useful for increasing feed digestibility. Cellulase is enzyme consisting of endoglucanase, exoglucanase and β-glucosidase enzymes that function to convert feed cellulose into glucose (Pukan et al 2015;Nababan et al 2019;Olukunle et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics

et al. 2022

View full text Add to dashboard Cite

Abstract. Agustina S, Wiryawan KG, Suharti S, Meryandini A. 2022. Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics. Biodiversitas 23: 6448-6455. Anaerobic fungi are essential microbes in the degradation process of feed fiber in the rumen with the ability to produce fibrolytic enzymes and rhizoids that can penetrate feed particles. The activity of cellulase enzymes in fungi is influenced by several factors such as the type of feed and livestock used as a source of isolate. Therefore, this research aimed to analyze the types of anaerobic fungi isolated from buffalo based on their DNA nitrogenous bases composition and also to evaluate the growth rate, cellulase enzyme activity, and fermentation product concentration of anaerobic fungi. The growth rate of fungi was examined by measuring the biomass formed, while cellulase enzyme activity was carried out using CMC, Avicel, and Filter Paper as substrates. The fungal fermentation products were analyzed using the HPLC and GC methods. The results showed that the fungi isolated from buffalo rumen were closely related to anaerobic fungi type Piromyces sp., Caecomyces sp., and Neocallimastix frontalis, with different growth rate, cellulase enzyme activity, and the fermentation products concentration in each type of anaerobic fungi. Therefore, it can be concluded that anaerobic fungi isolated from buffalo rumen can degrade cellulose. It was also discovered that fungi Neocallimastix frontalis had a higher growth rate, cellulase enzyme activity (CMCase, avicelase, and FPase), and fermentation products than Piromyces sp. and Caecomyces sp.

show abstract

“…SSF has high productivity in short periods, facilitated oxygen circulation, simpler technology, reduced operational problems, and resembles the natural habitat of several microorganisms. The enzymes produced are less susceptible to substrate inhibition problems and are stable against changes of temperature and pH, in addition to having some environmental importance, since SSF has the ability to use substrates from agro-industrial residues, which serve as a source of carbon and energy for enzymatic growth (Almanaa et Carboxymethyl cellulases consist of a multi-enzyme complex comprising endoglucanases, exoglucanases, and β-glucosidases which act together to completely hydrolyze the cellulose molecule (Olukunle et al 2021). The global enzyme market for cellulases is signi cant due to the possibility of their use in many industrial processes such as those involving textiles, laundry detergents, paper and pulp, brewing and wine, and biomass re neries (Olukunle et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Stabilization of carboxymethyl cellulase from the fungus Aspergillus niger produced by solid-state fermentation in fruit residues using chemical preservatives.

Lima

Costa

Franco

et al. 2023

Preprint

View full text Add to dashboard Cite

The residues of fruticulture of fruits such as acerola, guava, passion fruit, and mango can be efficiently used to produce enzymes by solid-state fermentation. In this work, we used these residues and the filamentous fungus Aspergillus niger ATCC 1004 to obtain carboxymethyl cellulase (CMCase). The preservation of enzyme activity is fundamental for providing commercial enzymes with a good price and high enzyme activity. Three substances (sodium chloride, sodium benzoate, and monosodium phosphate) were tested as enzyme activity preservatives; a simplex-centroid mixture design was applied to obtain the best concentrations of these substances. The effect of preservatives on the activity of the CMCase enzyme was tested for 72 hours by incubating the enzyme with the salts of sodium chloride, monosodium phosphate, and sodium benzoate at various concentrations, in citrate-phosphate buffer pH 5.0 at 50 mM, at a temperature of 50°C. The results showed that the mixture that produced the optimum response was composed of 42% sodium chloride, 38% sodium benzoate, and 20% monosodium phosphate. Then, the substances studied shows that are good option to preserve the CMCase enzyme produced by the fungus Aspergillus niger ATCC 1004.

show abstract

Carboxymethyl Cellulase (CMCase) from UV-irradiation Mutated Bacillus cereus FOA-2 cultivated on Plantain (Musa parasidiaca) Stalk-based Medium: Production, Purification and Characterization

Cited by 10 publications

References 34 publications

Isolation and Identification of Bacteria of Genus Bacillus from Composting Urban Solid Waste and Palm Forest in Northern Peru

Isolation and Identification of Bacteria of Genus Bacillus from Composting Urban Solid Waste and Palm Forest in Northern Peru

Molecular identification of anaerobic fungi isolated from buffalo rumen with their growth rate, cellulase enzyme activity, and fermentation products characteristics

Stabilization of carboxymethyl cellulase from the fungus Aspergillus niger produced by solid-state fermentation in fruit residues using chemical preservatives.

Contact Info

Product

Resources

About