Carboxymethylated degraded polysaccharides from Enteromorpha prolifera: Preparation and in vitro antioxidant activity

Shi, Mei-Jia; Wei, Xiaoyi; Xu, Jie; Chen, Bing-Jie; Zhao, Deyin; Cui, Shuai; Zhou, Tao

doi:10.1016/j.foodchem.2016.07.151

Cited by 113 publications

(75 citation statements)

References 34 publications

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“…As the concentration increased, the hydroxyl radical scavenging activity of DIP‐1 increased gradually ranging from 24.57% to 77.27% (Figure a). The EC 50 (concentration for 50% of maximal effect) values of DIP‐1 for scavenging hydroxyl radical was 1.07 mg/ml, that exceeded Vc (EC 50 = 0.87 mg/ml), but lower than that from Enteromorpha prolifera polysaccharide (PE, EC 50 = 6.2 mg/ml) (Shi et al, ). The scavenging effects of DIP‐1 and Vc against hydroxyl radical were determined to be 77.27 ± 2.37% and 85.29 ± 0.42% at a maximum concentration (1.50 mg/ml).…”

Section: Resultsmentioning

confidence: 99%

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Xiang

Лі

et al. 2019

J Food Biochem

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A neutral polysaccharide (DIP‐1) from Duchesnea indica (Andr.) Focke was obtained by hot water extraction, ethanol precipitation and chromatographic separation (DEAE‐52 cellulose anion‐exchange column and Sephadex G‐100 gel column). The physicochemical properties of DIP‐1 were elucidated by gel permeation chromatography, monosaccharide composition, Fourier transform infrared spectrometry, UV–visible spectrophotometry, scanning electron microscope and Congo red test. The results indicated that DIP‐1 was consisted of mannose, glucosamine, glucose, galactose and arabinose in a ratio of 1.00:0.42:18.36:14.17:0.81, and its molecular weight was 218.3 kDa. Meanwhile, DIP‐1 presented a straight hexahedron structure, but no triple‐helical conformation. In antioxidant activity tests, DIP‐1 exhibited powerful scavenging activities on hydroxyl, DPPH, ABTS radicals and reducing power in a dose‐dependent manner. Especially, DIP‐1 demonstrated high inhibitory activities against SKOV‐3 and Hep‐G2 cells in vitro, with IC50 values of 1.42 and 1.23 mg/ml, respectively. Practical applications D. indica has been used for a long time as a Chinese medicine for therapy of many diseases, including cancer, inflammation, leprosy, fever, bleeding and so on. At present, polysaccharides have attracted comprehensive attention because of a large range of pharmacological and biological properties, including antitumor, antidiabetic, antioxidant and immunomodulatory activity. In the present study, we purified and characterized a neutral polysaccharide from D. indica for the first time. Moreover, the neutral polysaccharide exhibits significant antioxidant and antitumor activities. Therefore, the present study laid a foundation for the high‐value application of D. indica polysaccharides in functional food and pharmaceutical industries.

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Section: Resultsmentioning

confidence: 99%

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Xiang

Лі

et al. 2019

J Food Biochem

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“…Shi et al . 13 reported a polysaccharide from E . prolifera (Zhejiang, China), which was composed of rhamnose (67.8%), glucose (18.6%), xylose (7.7%), galactose (4.0%), and mannose (1.4%).…”

Section: Resultsmentioning

confidence: 99%

Directional preparation of anticoagulant-active sulfated polysaccharides from Enteromorpha prolifera using artificial neural networks

Cui

Wang

et al. 2018

Sci Rep

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The sulfated polysaccharides from Enteromorpha prolifera (PE) are a potential source of anticoagulant agents. In this study, the PE was degraded by specific degradase and five hydrolysis products with different molecular weights were prepared. The product of 206 kDa is a kind of high rhamnose-containing polysaccharide with sulfate ester (34.29%). It could effectively prolong the activated partial thromboplastin time (APTT), which indicated inhibition of the intrinsic coagulation pathway. The artificial neural network (ANN) was built to realize the directional preparation of anticoagulant-active polysaccharides. Based on monitoring glucose concentration on-line, a visualization system of enzymatic hydrolysis was developed to simplify the operation of ANN. The model could be further applied to predict molecular weights of polysaccharides that possess diverse biological activities.

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“…The peak at around 1241 cm −1 was a symmetrical carbonyl stretching. The absorption peak at 1036 cm −1 was the characteristic peak of pyranoside and the weak small peak at 904 cm −1 indicated β -glucosidic bonds in TFPI [21]. All the structural characteristics of TFPI were quite different from the structure of TPP-1 previously reported by Chen et al [2].…”

Section: Resultsmentioning

confidence: 96%

Polysaccharides fromTrichosanthes Fructusvia Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

Chen

Shen

et al. 2017

BioMed Research International

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An efficient procedure for ultrasound-assisted enzymatic extraction of crude polysaccharides from Trichosanthes Fructus (crude TFP) using response surface methodology (RSM) was developed. The Box–Behnken design was applied to optimize the effects of pH (X1), enzyme amount (X2), extraction temperature (X3), and liquid-to-solid ratio (X4) on the extraction. The statistical analysis indicated that the independent variables (X4, X2, and X3), the quadratic coefficients (X12, X22, X32, and X42), and the interaction coefficient (X1X3) had significant impact on the yield of crude TFP. The optimal conditions were determined as follows: pH 4.5, enzyme amount 5000 u/g, extraction temperature 45°C, and liquid-to-solid ratio 30 ml/g. The experimental yield of crude TFP was 6.58%, which was very close to the predicted yield of 6.71%. TFPI was then purified and characterized with Sephadex G-100 column, UV-Vis, GPC, and FT-IR. The average molecular weight of TFPI was calculated to be 1.49 × 105 Da. TFPI exhibited strong reducing power and possessed not only remarkable scavenging activities against ABTS•+ and DPPH radicals, but also high antitumor activities in C4-2, DU145, and PC3 cells. The results suggest that Trichosanthes Fructus and TFPI could be a novel potent natural medicine with antioxidant and antitumor activities.

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Carboxymethylated degraded polysaccharides from Enteromorpha prolifera: Preparation and in vitro antioxidant activity

Cited by 113 publications

References 34 publications

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Characterization, antioxidant, and anticancer activities of a neutral polysaccharide from Duchesnea indica (Andr.) Focke

Directional preparation of anticoagulant-active sulfated polysaccharides from Enteromorpha prolifera using artificial neural networks

Polysaccharides fromTrichosanthes Fructusvia Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

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