Carboxymethylation of polysaccharide from Morchella angusticepes Peck enhances its cholesterol-lowering activity in rats

Yao, Li; Yuan, Ye; Lei, Lin; Li, Fuhua; Zhang, Yueqiao; Chen, Jinlong; Zhao, Guohua; Wu, Shafei; Yin, Rong

doi:10.1016/j.carbpol.2017.05.033

Cited by 52 publications

(26 citation statements)

References 32 publications

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“…Nevertheless, the hydrophobicity of melanin may limit its applications. It was reported that its water solubility could be improved by amino acid [24], carboxymethylation [38], d ‐glucosamine [25], and methylation [39]. In the present study, GLM was modified by a series of amino acids.…”

Section: Discussionmentioning

confidence: 79%

Comparison of physicochemical and biochemical properties of natural and arginine‐modified melanin from medicinal mushroom Ganoderma lucidum

Li²,

Chang

et al. 2020

J Basic Microbiol

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Melanin is a hydrophobic biomolecule produced widely in fungi. Compared with other fungi, health benefits have been associated with medicinal mushrooms, which may provide an excellent source of natural melanin. Nevertheless, the hydrophobicity of melanin may limit its applications. Consequently, the present study was carried out on isolation of melanin from the medicinal mushroom Ganoderma lucidum (GLM) and modification with arginine to improve its solubility. The physicochemical and biochemical properties of melanin were evaluated including structural characterization, solubility, stability, antioxidant activities, and inhibitory effect on pancreatic lipase activity. Arginine‐modified melanin showed better solubility, higher color value, stronger antioxidant activity, and stronger inhibitory effect on pancreatic lipase activity in vitro than GLM. In addition, both have good stability in the dark and natural light. These results opened possibilities for providing an excellent source of natural melanin in health food or food additives fields.

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Section: Discussionmentioning

confidence: 79%

Comparison of physicochemical and biochemical properties of natural and arginine‐modified melanin from medicinal mushroom Ganoderma lucidum

Li²,

Chang

et al. 2020

J Basic Microbiol

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“…The chemical modification is an important way for polysaccharides to improve their intrinsic bioactivities and create new functional properties (Nie, Shi, Ding, & Tao, ). The main chain of PMEP was composed of (1→4)‐α‐D‐glucose, (1→6)‐α‐D‐galactose, (1→2)‐α‐D‐mannose, and (1→5)‐α‐D‐arabinose; its branch chain was composed of (1→2→6)‐α‐D‐mannose, (1→2→6)‐α‐D‐glucose, and (1→2→6)‐α‐D‐galactose (Li et al., ). Hence, we prepared the sulfated, carboxymethylated, and acetylated derivatives of PMEP, and studied their antioxidant and antiproliferative activities.…”

Section: Discussionmentioning

confidence: 99%

“…The water‐soluble PMEP was extracted and purified according to our previous method (Li et al., ). The concentrated fractions were lyophilized and named as PMEP, storing in room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…The extracellular polysaccharides from Pleurotus sajor‐caju did not show anticoagulant or antiproliferative activities, but the sulfated derivatives demonstrated promising antioxidant, antiproliferative, and anticoagulant properties (Telles et al., ). Our previous study reported that the carboxymethylation of polysaccharides extracted from Morchella angusticepes Peck (PMEP) was an efficient way to improve the cholesterol‐lowering activity in vivo (Li et al., ). The literature available on cellular antioxidant activity (CAA) of modified polysaccharides is very scarce.…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant and Antiproliferative Activities of Modified Polysaccharides Originally Isolated from Morchella Angusticepes Peck

Tang

Chen

et al. 2019

Journal of Food Science

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Polysaccharides extracted from Morchella angusticepes Peck (PMEP) were chemically modified to obtain sulfated, carboxymethylated, and acetyled derivatives. Results showed that the acetyled derivatives with the degree of substitution (0.40 ± 0.07) exerted higher antioxidant ability than native polysaccharides, demonstrating inhibitory effects on growth of human hepatoma cells (EC50 = 0.710 ± 0.002 mg/mL) and human colon cancer cells (EC50 = 1.229 ± 0.008 mg/mL). It indicated that the acetylation was a favorable way to enhance the bioactivities of PMEP. Practical Application Polysaccharides extracted from Morchella angusticepes Peck (PMEP) have many health‐promoting properties. Chemical modifications could improve the bioactivities of polysaccharides. We demonstrated that acetylation enhanced the cellular antioxidant and antiproliferative activities of PMEP. The results support further research that explores clinical utility, and may justify ex vivo and in vivo designs toward that end.

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“…The extra-polysaccharides as well as crude polysaccharides isolated from morels had a remarkable effect on alleviating oxidative stress [18,19]. Moreover, with cholesterol-lowering ability, morels protect against cardiovascular diseases [20]. In addition, it has more functions, like more exhibited hepatoprotective activity, resisting gentamicin and cisplatin induced nephrotoxicity by restoring the depleted antioxidant defense system [21,22].…”

Section: Introductionmentioning

confidence: 99%

The potential neuritogenic activity of aqueous extracts from Morchella importuna in rat pheochromocytoma cells

Luo

Huang

et al. 2017

Food Sci Biotechnol

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The aim of this study was to explore the neuritogenic effects of aqueous extracts from the fruiting bodies of (MEA). 3-(4, 5-dimethythiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay was carried out to assess the cytotoxicity of MEA. Neurite outgrowth stimulation assay was used to evaluate the potentiation of neuritogenic activity induced by MEA. The specific inhibitors for TrkA, MEK/ERK and PI3K signaling pathway were served to clarify the mechanism of MEA's neuritogenic effects. It was shown that MEA could mimic neuritogenic activity of NGF, a kind of representative neurotrophic factors with no significant cytotoxicity, and stimulate neurite outgrowth in a dose-dependent manner of PC12 cells. The neuritogenic activity induced by MEA required activity of PI3K/Akt and MEK/ERK1/2 signaling pathways, as well as parts of TrkA receptor. Accordingly, MEA could be used as a promising neuritogenic-stimulation compound for nervous diseases treatment.

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Carboxymethylation of polysaccharide from Morchella angusticepes Peck enhances its cholesterol-lowering activity in rats

Cited by 52 publications

References 32 publications

Comparison of physicochemical and biochemical properties of natural and arginine‐modified melanin from medicinal mushroom Ganoderma lucidum

Comparison of physicochemical and biochemical properties of natural and arginine‐modified melanin from medicinal mushroom Ganoderma lucidum

Antioxidant and Antiproliferative Activities of Modified Polysaccharides Originally Isolated from Morchella Angusticepes Peck

The potential neuritogenic activity of aqueous extracts from Morchella importuna in rat pheochromocytoma cells

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