Carboxymethylpachymaran-zein coated plant microcapsules-based β-galactosidase encapsulation system for long-term effective delivery

Deng, Zhao; Wang, Shishuai; Zhou, Bin; Li, Jing; Zhou, Peiyuan; Li, Bin; Liang, Hongshan

doi:10.1016/j.foodres.2019.108867

Cited by 20 publications

(15 citation statements)

References 35 publications

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“…The SECs were extracted by the procedure reported in our previous work. 28 Briefly, pollen grains were mixed with phosphoric acid for 5 h at 70 °C, which were then washed with water, acetone, hydrochloric acid, and ethanol sequentially.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…2,23 Accordingly, because of their unique properties and costeffective production, SECs have been regarded as a promising candidate for the oral delivery of protein or peptide drugs, which can protect the drugs from the harsh environment in the stomach, and achieve a prolonged and targeted release in the small intestine. 27,28 However, one major challenge is the presence of numerous pores on the surface of SECs, which can cause the leakage of the loaded bioactive macromolecules. 25 Thus, some researchers have attempted to employ polymer encapsulation to address this limitation.…”

Section: ■ Introductionmentioning

confidence: 99%

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Designable Carboxymethylpachymaran/Metal Ion Architecture on Sunflower Sporopollenin Exine Capsules as Delivery Vehicles for Bioactive Macromolecules

Deng

Pei

Wang

et al. 2020

J. Agric. Food Chem.

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There are multiple obstacles in the gastrointestinal tract (GIT) for oral administration of bioactive macromolecules. Here, we engineered an oral delivery vehicle (sporopollenin exine capsules with carboxymethylpachymaran (CMP)/metal ion modification) with targeted release based on food-grade ingredients and processing operations. Then, the interaction and binding mechanisms between CMP and metal ions in the vehicle were investigated. By using β-galactosidase (β-Gal) as a model protein, the systems were characterized for the surface morphology and monitored by the in vitro release profile of β-Gal. Notably, the CMP/ metal ion systems not only markedly decreased the CMP dosage but also achieved a valid long-term release compared with the previously reported CMP system. Among all the systems, the CMP/3% AlCl 3 system showed the best ability to control the release with the maximum residual activity of β-Gal at nearly 72% after 24 h of treatment. Subsequently, the interaction mechanism between CMP and metal ions within the system was characterized by the perspectives of microstructure, rheological properties, and spectroscopy characteristics. The results indicated that the low pH conditions are conducive to the further cross-linking of CMP and metal ions, resulting in a high gel strength and thus a dense structure, which can impact the controlled release of β-Gal in the GIT. Overall, the system may be utilized in the administration of medical and functional foods, specifically for the delivery of bioactive proteins via the oral route.

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Section: ■ Materials and Methodsmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Designable Carboxymethylpachymaran/Metal Ion Architecture on Sunflower Sporopollenin Exine Capsules as Delivery Vehicles for Bioactive Macromolecules

Deng

Pei

Wang

et al. 2020

J. Agric. Food Chem.

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“…SECs were obtained according to the previous procedure. 31 After acid hydrolyzation, the pollen grains were washed with organic reagents.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

“…27,28 Chemical modification has been used to solve this problem based on the covalent or noncovalent interaction between zein and other compounds (polysaccharides, lipids, and small molecules), which can improve the performance of zein when used in an oral delivery system. 29,30 Previously, we have fabricated a zein/polysaccharide system as an orally administered drug carrier based on the noncovalent interaction between zein and CMP, 31 but oral delivery systems with a low cost and a sustained small intestinal release of bioactive macromolecules are still not available. Great effort has been made to utilize proteins and polyphenols to construct delivery systems due to their interactions.…”

Section: ■ Introductionmentioning

confidence: 99%

“…It is commonly known that the main source of zein is industrial scraps, and its utilization can not only meet the need of sustainable industrial production but also be cost-effective. , As an alcohol-soluble protein, zein possesses many unique characteristics and can be converted into films and nanoparticles to be applied in oral delivery systems . However, the application of zein is largely hindered by its susceptibility to variable solubility near the isoelectric point. , Chemical modification has been used to solve this problem based on the covalent or noncovalent interaction between zein and other compounds (polysaccharides, lipids, and small molecules), which can improve the performance of zein when used in an oral delivery system. , Previously, we have fabricated a zein/polysaccharide system as an orally administered drug carrier based on the noncovalent interaction between zein and CMP, but oral delivery systems with a low cost and a sustained small intestinal release of bioactive macromolecules are still not available. Great effort has been made to utilize proteins and polyphenols to construct delivery systems due to their interactions.…”

Section: Introductionmentioning

confidence: 99%

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Tuning of Molecular Interactions between Zein and Tannic Acid to Modify Sunflower Sporopollenin Exine Capsules: Enhanced Stability and Targeted Delivery of Bioactive Macromolecules

Deng

Wang

Pei

et al. 2021

ACS Appl. Bio Mater.

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There are multiple obstacles for the storage and digestion of orally administered bioactive macromolecules. This study developed a low-cost and sustained-release delivery system (sporopollenin exine capsules with zein/tannic acid modification) of proteins with excellent storage stability, and at the same time provided insights into the sustained-release mechanism through exploring the interaction between zein and tannic acid (TA). β-Galactosidase (β-Gal) was utilized as a model protein and loaded into sporopollenin exine capsules (SECs), which were then coated with the zein/TA system. Under the optimized zein/TA conditions, the zein/TA system showed better performance than the zein alone system in the sustained release of β-Gal, with the residual activity of about 70.26% after 24 h of simulated digestion. Evaluation of the storage stability demonstrated a β-Gal residual activity of nearly 90% for 28 days at 25 °C. Additionally, FTIR analysis demonstrated that the stability of the zein/TA system depends on both hydrogen bonding and certain covalent bonding through the Schiff-base reaction, and the sustained release is regulated by the bonding strength.

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Sporopollenin Spikes Augment Antigen‐Specific Immune Response and Generate Long‐Lived Humoral Immunity

Uddin

Gonzalez-Cruz

Warzywoda

et al. 2020

Advanced Therapeutics

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Oral vaccine delivery remains an unmet goal due to biochemical and immunological barriers in the gastrointestinal tract. Sporopollenin microcapsules from natural pollen grains have recently been engineered to overcome these multifaceted challenges. Using four morphologically different sporopollenin shells and two inbred mouse strains, this study addresses three key questions regarding sporopollenin shell's application for oral vaccine delivery: i) the impact of sporopollenin shell surface morphology on the immune response, ii) the duration of the immunity, and iii) the applicability of the delivery system across a diverse genetic background population. Using ovalbumin (OVA) as a model vaccine antigen, this study demonstrates that OVA can adsorb on the sporopollenin shell surfaces. Mice orally vaccinated with a sporopollenin shell-based OVA formulation show sustained antibody responses for 454 days after the immunization that are correlated with the generation of OVA-specific plasma cells in the vaccinated mice bone marrow. Sporopollenin shell surface spikes have a greater impact on immune responses than the shell size and shape. A spiky ragweed sporopollenin formulation induces systemic and mucosal responses in C57BL/6 and BALB/c mice. Together, this study provides a framework to select sporopollenin shells based on the surface morphology to use as a microcapsule for oral vaccination.

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Carboxymethylpachymaran-zein coated plant microcapsules-based β-galactosidase encapsulation system for long-term effective delivery

Cited by 20 publications

References 35 publications

Designable Carboxymethylpachymaran/Metal Ion Architecture on Sunflower Sporopollenin Exine Capsules as Delivery Vehicles for Bioactive Macromolecules

Designable Carboxymethylpachymaran/Metal Ion Architecture on Sunflower Sporopollenin Exine Capsules as Delivery Vehicles for Bioactive Macromolecules

Tuning of Molecular Interactions between Zein and Tannic Acid to Modify Sunflower Sporopollenin Exine Capsules: Enhanced Stability and Targeted Delivery of Bioactive Macromolecules

Sporopollenin Spikes Augment Antigen‐Specific Immune Response and Generate Long‐Lived Humoral Immunity

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