“…In recent years, several studies aiming to evaluate the effects of feed (Fugita et al, 2012;Zawadzki et al, 2011;Valero et al, 2011;Sun et al, 2009;Fernandes et al, 2009;Jorge et al, 2009;Kazama et al, 2008;Silva et al, 2002;Prado et al, 2008b), genetic groups (Ito et al, 2012;Maggione et al, 2009;Rossato et al, 2009;Ducatti et al, 2009;Oliveira et al, 2009;Orellana et al, 2009;Abrahão et al, 2008;Prado et al, 2008a;2008c;2008d), sex (Prado et al, 2009a;2009b;Aricetti et al, 2008, Kuss et al, 2006, slaughter age (Ito et al, 2010), effect of a Luteinizing Hormone-Releasing Hormone (LHRH) fusion protein vaccine vs. surgical castration (Ruiz et al, 2005) and the anatomical location of muscles on meat quality in cattle, quantified the total fat, using gravimetric methods. It has been observed that, in general, if the objective is, beyond the quantification of total lipids, the quantification of fatty acids of meat lipids, mainly of polyunsaturated lipids, the methods used by researchers involve extracting lipids and sampling the "fresh" ones with solvents at room temperature.…”