Carcinoembryonic antigen and blood group-related carbohydrate antigens in glycoproteins in human bile in hepatolithiasi

Abstract

We investigated whether carbohydrate antigens on cholangitis, 2,4 in which the proliferated peribiliary biliary glycoproteins and carcinoembryonic antigen glands secrete large amounts of acid (sialylated and (CEA) are related to hepatolithiasis. CEA, ABO, and sulfated) or neutral mucin into the biliary lumen. 4 Most Lewis blood group-related antigens, as well as sialylintrahepatic calculi consist of calcium-bilirubinate Tn antigen in hepatic bile, were analyzed by Western (brown pigment) stones. 4,5 Bacterial infection, bile stablotting in samples from 12 patients with hepatolithiasis sis, and an alteration of the bile composition are and 37 with other biliary diseases (choledocholithiasis, thought to be responsible for the nucleation, formation, 13; cholecystolithiasis, 5; acute cholecystitis, 2; choland maturation of intrahepatic calculi. 6,7 In addition, angiocarcinoma, 5; common bile duct carcinoma, 4; panthe increased mucin production and secretion from the creatic carcinoma, 6; and metastatic carcinoma of liver, bile ducts may play an important role in the develop-2). CEA was positive on mucinous glycoprotein in six patients (50%) with hepatolithiasis and one case (17%) ment of intrahepatic calculi. 4,5,8 Biliary mucin, particuwith pancreatic carcinoma. CEA was also positive on a larly sulfated, reportedly promotes the formation of calglycoprotein of approximately 200 kd in eight patients cium-bilirubinate stones. 4,7 Histochemically, these (67%) with hepatolithiasis and two (33%) with pancreatic stones are composed of alternating multilayers of mucarcinoma. Lewis X was detected on the mucinous glycocin and pigment (calcium-bilirubinate) on their cut surprotein in almost all samples, as well as on glycoproteins face, suggesting that mucin bridges the pigment layers of approximately 180 kd in all hepatolithiasis samples and participates in the precipitation of calcium-biliruand approximately half of those from patients with other binate. 9 diseases. Sialyl-Tn antigen was detected on mucinousCarbohydrate antigens of glycoproteins, glycolipids, glycoprotein in four (80%) with cholangiocarcinoma, two and proteoglycans play an important role in cell-to-

Expression of sialyl‐Tn, Tn and T antigens in primary liver cancer

“…The frequent neoexpression of STn antigen and overexpression of Tn antigen in CC are consistent with a previous immunohistochemical study 28 . This finding also agrees with a previous report describing that STn antigen on mucin was frequently and specifically detected in bile samples from patients with CC and pancreatic cancer 32 . T antigen was expressed focally in peribiliary glands in non‐neoplastic tissue and in about half of the cases of CC without cirrhosis.…”

Relationship between interleukin‐6 and proliferation and differentiation in cholangiocarcinoma

“…Western blotting was carried out with a rabbit polyclonal primary antibody against human IL-6 (R & D System) as described previously. 20 Briefly, bile samples (50 l) were solubilized at 100ЊC for 5 min in 50 l sample buffer (50 mmol/l TRIS/HCl (pH 6.8), 2% sodium dodecyl sulphate (SDS), 10% 2mercaptoethanol, 0.1% bromophenol blue and 1% glycerol). Protein in bile was separated by means of 15% to 25% gradient SDS polyacrylamide gel electrophoresis (MULTI GEL, Daiichi Pure Chemicals, Tokyo, Japan) at 40 mA for 60 min.…”

Cholangiocarcinoma: Recent progress. Part 2: Molecular pathology and treatment

“…Increased apoptosis of host immunological cells by carcinoma cells is thought to play a key role in this process. Recently, it was shown that a tumor evasion mechanism involving Fas / FasL exists in intrahepatic CC; frequent and strong expression of FasL in biliary dysplasia, and well‐differentiated intrahepatic CC enables them to escape from immune surveillance by counter‐attacking Fas ‐bearing infiltrating lymphocytes 51 . The finding that infiltrating lymphocytes were more frequently apoptotic within intrahepatic CC foci than in non‐neoplastic foci remote from intrahepatic CC foci, also supports this hypothesis.…”