Carcinoembryonic antigen and blood group-related carbohydrate antigens in glycoproteins in human bile in hepatolithiasi

Abstract

We investigated whether carbohydrate antigens on cholangitis, 2,4 in which the proliferated peribiliary biliary glycoproteins and carcinoembryonic antigen glands secrete large amounts of acid (sialylated and (CEA) are related to hepatolithiasis. CEA, ABO, and sulfated) or neutral mucin into the biliary lumen. 4 Most Lewis blood group-related antigens, as well as sialyl-intrahepatic calculi consist of calcium-bilirubinate Tn antigen in hepatic bile, were analyzed by Western (brown pigment) stones. 4,5 Bacterial infection, bile stablotting in samples from 12 patients with hepatolithiasis sis, and an alteration of the bile composition are and 37 with other biliary diseases (choledocholithiasis, thought to be responsible for the nucleation, formation, 13; cholecystolithiasis, 5; acute cholecystitis, 2; chol-and maturation of intrahepatic calculi. 6,7 In addition, angiocarcinoma, 5; common bile duct carcinoma, 4; panthe increased mucin production and secretion from the creatic carcinoma, 6; and metastatic carcinoma of liver, bile ducts may play an important role in the develop-2). CEA was positive on mucinous glycoprotein in six patients (50%) with hepatolithiasis and one case (17%) ment of intrahepatic calculi. 4,5,8 Biliary mucin, particuwith pancreatic carcinoma. CEA was also positive on a larly sulfated, reportedly promotes the formation of calglycoprotein of approximately 200 kd in eight patients cium-bilirubinate stones. 4,7 Histochemically, these (67%) with hepatolithiasis and two (33%) with pancreatic stones are composed of alternating multilayers of mucarcinoma. Lewis X was detected on the mucinous glyco-cin and pigment (calcium-bilirubinate) on their cut surprotein in almost all samples, as well as on glycoproteins face, suggesting that mucin bridges the pigment layers of approximately 180 kd in all hepatolithiasis samples and participates in the precipitation of calcium-biliruand approximately half of those from patients with other binate. and proteoglycans play an important role in cell-to-(50%) with common bile duct carcinoma, and all pancreatic carcinoma samples. Mucinous glycoprotein and gly-cell adhesion and recognition 10-13 and also have a close coproteins containing CEA and Lewis X antigens are en-relationship with a specific bacterial infection.14,15 Carriched in hepatic bile of hepatolithiasis, and they may be cinoembryonic antigen (CEA), which is widely used as closely related to the formation of intrahepatic calculi. a tumor marker, 16 also functions as an intercellular Sialyl-Tn antigen in biliary mucinous glycoprotein will adhesion molecule 17,18 and also binds and aggregates be a good marker of biliary and pancreatic carcinoma, bacteria. 19,20 Immunohistochemical studies 21-23 have and probably for cholangiocarcinoma complicated with disclosed that the biliary epithelial cells at different hepatolithiasis. (HEPATOLOGY 1996;23:258-263.) anatomic levels have a characteristic distribution of carbohydrate antigens, and their altered distribution Hepatolithiasis is quite common in the Fa...

Serum sialyl Lewis X-i antigen in lung adenocarcinoma and idiopathic pulmonary fibrosis

“…In both lung adenocarcinoma and non-malignant lung disease the antigen carries glycoproteins in broad bands with a polydispersed pattern which is usually observed in Western blotting analysis of glycoproteins. [26][27][28][29] Figures 1 and 2 show the results of Western blotting. In each group of patients with the same diagnosis the sialyl Lewis X-i antigen at a level >50 U/ml essentially had the same pattern.…”

“…[26][27][28][29] The variations in molecular size are now thought to be due to length polymorphism of the gene encoding for apomucin. 30 Mucins are glycoproteins of high molecular weight which contain a large amount of carbohydrate O-linked to protein through serine and threonine.…”

Secretory Low-Molecular–Weight Phospholipases A2 and Their Specific Receptor in Bile Ducts of Patients With Intrahepatic Calculi: Factors of Chronic Proliferative Cholangitis

“…Importantly, gel-forming mucins of MUC2 and MUC5 (here MUC5AC) could be pathogenetically involved in the formation and growth of intrahepatic calculi. Because acid (sialylated 27 and sulfated 28 ) mucins are reportedly associated with the formation of brown pigment stones, 8,28 interest should be focused on the presence of a certain mucin core protein that is preferred for the formation of sialylated or sulfated carbohydrate epitope. However, because of the heterogeneous and aberrant expression of mucin core polypeptide genes in intrahepatic calculi, 73 we were unable to come to any definite conclusion.…”

“…The proliferated glands were seen to secrete large amounts of acid (sialylated 27 and sulfated 28 ) mucins (high-molecular-weight glycoprotein) into the dilated lumen. 9 Excess amounts of mucin secreted into the inner surface of the ducts may provide a microenvironment initiating a nidus for stones by trapping calcium salts 29 of bilirubin, phosphate, carbonate, and lipids.…”