Carcinogen 7,12-dimethylbenz[a]anthracene-induced mammary tumorigenesis is accelerated in Smad3 heterozygous mice compared to Smad3 wild type mice

Liu, Zhengxue; Kundu-Roy, Tanima; Matsuura, Isao; Wang, Guannan; Lin, Yong; Lou, You-Rong; Barnard, Nicola; Wang, Xiao Fan; Huang, Mou Tuan; Suh, Nanjoo; Liu, Fang

doi:10.18632/oncotarget.11713

Cited by 7 publications

(5 citation statements)

References 28 publications

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“…In this context, 7,12‐dimethylbenzanthracene (DMBA) is a prokaryogen, which requires metabolic activation by cytochrome P450 (CVYP1A2 and CYP1A1, CYP1B1) enzymes to generate the metabolite dihydrodiolepoxide, which can generate DNA adducts in peripheral blood and breast tissue . It induces BC in animals by double strand breaks of DNA subsequent damage repair .…”

Section: Introductionmentioning

confidence: 99%

Phytol as an anticarcinogenic and antitumoral agent: An in vivo study in swiss mice with DMBA‐Induced breast cancer

et al. 2018

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Phytol (PHY) (3,7,11,15‐tetramethylhexadec‐2‐en‐1‐ol) exhibits various pharmacological properties including toxicity and cytotoxicity, and exerts antitumor activity. Owing to the urgent need of new pharmaceutical formulations for breast cancer therapy, this study aimed at the evaluation of antitumor activity of PHY in 7,12‐dimethylbenzanthracene‐cancer‐induced animal model. Comet assay was employed to evaluate the cytogenetics, DNA repair, and antigenotoxic activities of PHY in neoplastic (breast) and non‐neoplastic rodent cells (bone marrow, lymphocytes, and liver). Additionally, hematological, biochemical, histopathological, and immunohistochemical analyses were carried out in experimental animals. Thirty nonpregnant female mice (n = 5) underwent 7 weeks treatment with 6 mg/kg pro‐carcinogen, PHY (4 mg/kg), and cyclophosphamide (25 mg/kg). Induction of cancer was confirmed by histopathology and immunohistochemistry for Ki‐67. Results suggest that PHY exhibits low toxicity in comparison with other groups in hematological, biochemical, histopathological, and organ size parameters. Additionally, PHY showed modulatory effects on the pro‐carcinogen, and induced genotoxicity and apoptosis in breast cancer cells. Furthermore, it showed a DNA damage repair capacity in mouse lymphocytes. These data indicate that PHY may have the potential as an anticancer candidate in pharmaceutical consumption. © 2018 IUBMB Life, 71(1):200–212, 2019

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Section: Introductionmentioning

confidence: 99%

Phytol as an anticarcinogenic and antitumoral agent: An in vivo study in swiss mice with DMBA‐Induced breast cancer

et al. 2018

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“…The induction of DMBA and Estradiol carcinogen compounds for 8 weeks induced the formation of breast cancer 7,12-Dimethylbenz[a]anthracene (DMBA) was a carcinogen compound that has an aromatic hydrocarbon chain and has been widely used in research as an inducer in breast cancer modeling [22,23,24]. Estradiol is a steroid the sex hormone that useful in female reproductive cycle in regulation of estrous and menstrual cycles.…”

Section: Resultsmentioning

confidence: 99%

The Effect of E. scaber Extract to TNF-α and TGF-β on Mice under Carcinogen Treatment

Amalia

Widyarti

Djati

2018

JELS

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Carcinogen compounds are the compounds that cause DNA damage and trigger cancer. TNF-α and TGF-β are cytokines produced by immune cells and serve to maintain body homeostasis. Elephantopus scaber is a plant that prevents the cancer progression, and improve the body's immune system, so this study was conducted to determine the effect of E. scaber extract on TNF-α and TGF-β after the administration of carcinogen compounds. The study was conducted by administering the carcinogen compound DMBA with a dosage of 0.56 mg kg-1 BW and estradiol with a dose of 0.0504 mg kg-1 BW which was given alternately for 8 weeks to the Mus musculus test animals. The study was conducted in 3 groups: K-(the normal group of mice), K + (a carcinogen-induced group of mice), and P (a group of carcinogen-induced mice and E. scaber extract). The treatment was done in 1 week, 2 weeks, and 3 weeks. Immune cells were isolated from splenocytes and performed immunostaining for flow cytometry analysis. The computed relative amount is TNF-α produced by macrophages CD11b and TGF-β produced by Treg CD4CD25. The relative number of cells was analyzed by two-way ANOVA and advanced Tukey test with 95% confidence level (α = 0.05). The results showed no significant differences in the number of cytokines TNF-α and TGF-β in both the carcinogen-induced mice group and the mice group was given the extract of Elephantopus scaber for 1 week, 2 weeks and 3 weeks.

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“…DMBA induces mammary tumor ( Fig. 3 ) formation in an animal model [ 51 ]. Group 2 shows atypical features (pleomorphism and hyperchromasia) consistent with sarcomatoid lesions.…”

Section: Discussionmentioning

confidence: 99%

VEGFR-2 kinase domain inhibition as a scaffold for anti-angiogenesis: Validation of the anti-angiogenic effects of carotenoids from Spondias mombin in DMBA model of breast carcinoma in Wistar rats

et al. 2021

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Carcinogen 7,12-dimethylbenz[a]anthracene-induced mammary tumorigenesis is accelerated in Smad3 heterozygous mice compared to Smad3 wild type mice

Cited by 7 publications

References 28 publications

Phytol as an anticarcinogenic and antitumoral agent: An in vivo study in swiss mice with DMBA‐Induced breast cancer

Phytol as an anticarcinogenic and antitumoral agent: An in vivo study in swiss mice with DMBA‐Induced breast cancer

The Effect of E. scaber Extract to TNF-α and TGF-β on Mice under Carcinogen Treatment

VEGFR-2 kinase domain inhibition as a scaffold for anti-angiogenesis: Validation of the anti-angiogenic effects of carotenoids from Spondias mombin in DMBA model of breast carcinoma in Wistar rats

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